Precision of 655 nm Confocal Laser Profilometry for 3D surface texture characterisation of natural human enamel undergoing dietary acid mediated erosive wear

Mullan, Francesca; Mylonas, Petros; Parkinson, Charles; Bartlett, David; Austin, Rupert

doi:10.1016/j.dental.2017.12.012

Cited by 18 publications

(16 citation statements)

References 26 publications

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“…It should be mentioned, however, that in the present study we have not characterized the modified acquired enamel pellicle. Therefore, in future studies, proteomic and ultra‐structure analysis of the acquired enamel pellicle modified with these substances should be also performed, and the theory should be tested in native enamel, which presents different demineralization kinetics (23,37‐39).…”

Section: Discussionmentioning

confidence: 99%

The effect of red wine in modifying the salivary pellicle and modulating dental erosion kinetics

Carvalho

Niemeyer

Baumann

2020

European J Oral Sciences

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This study investigated the potential of red wine in modulating dental erosion kinetics in the presence or absence of salivary pellicle. Polished human enamel specimens were used in two conditions; presence or absence of acquired enamel pellicle; and subdivided according to exposure: red wine, orange juice, apple juice, or citric acid. The specimens were incubated in clarified whole human saliva (presence of acquired enamel pellicle) or in a humid chamber (absence of acquired enamel pellicle) for 2 h at 37°C, then in the test substances for 1 min, at 25°C, under shaking. This was repeated four times. Surface hardness was measured initially and after each cycle and surface reflection intensity was measured initially and after all cycles. In the presence of acquired enamel pellicle, red wine caused the least surface hardness loss, followed by orange juice, apple juice, and citric acid. Statistically significantly less surface reflection intensity loss was observed for red wine and orange juice than for apple juice and citric acid. In the absence of acquired enamel pellicle, red wine and orange juice caused less surface hardness loss than apple juice and citric acid. Orange juice showed the least surface reflection intensity loss, followed by red wine, citric acid, and apple juice. The polyphenol composition of these drinks can notably modulate the erosion kinetics.

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Section: Discussionmentioning

confidence: 99%

The effect of red wine in modifying the salivary pellicle and modulating dental erosion kinetics

Carvalho

Niemeyer

Baumann

2020

European J Oral Sciences

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“…A significant issue overcome in this study was in the localisation and characterisation of surface form change in the dental tooth model. Previous studies that utilised polished enamel samples and noncontacting measurement systems such as NCLP [1,9,19,21,26], confocal laser scanning microscopy (CLSM) [27,28], and vertical scanning interferometry (VSI) [29,30] have utilised single post-erosion scans to determine bulk enamel loss using a number of different surface form outcomes including mean single point step height [19,26,31] and mean 3D step height [1,2,32]. These outcomes are based on ISO 5436-1 which calculates step height according to the difference in height between the base of a given wear scar and one or two flat reference plane(s) either side of the wear scar [33].…”

Section: Discussionmentioning

confidence: 99%

“…The quantification of enamel loss using three-dimensional (3D) step height formation has been used on natural human enamel by using the profile subtraction method and analysing the residual data set [4]. This is a feat which was previously limited to research using artificially flattened enamel samples [8,9].…”

Section: Introductionmentioning

confidence: 99%

Surface form analysis on complex freeform organic structures – measuring erosive wear on human teeth in vitro

Mylonas

Moazzez

Joiner

et al. 2021

Surf. Topogr.: Metrol. Prop.

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Natural human enamel (NHE) is a complex freeform surface which has presented significant difficulties in measuring surface form change using non-contacting laser profilometry (NCLP). Measuring surface form change on NHE is a metrology proxy for measuring dental tooth structure loss, and characterising this using non-ISO parameters (volume, surface area, and normalised lesion depth) has been seldom studied due surface complexity and undetermined measurement errors. This study determines NCLP measurement errors (instrument repeatability and method reproducibility) for non-ISO parameters, characterises change in surface form on NHE following a dietary pH-cycling model. NHE (n = 1) was scanned consecutively twenty-times using NCLP with/without sample replacement producing consecutive surface profile data. Residual data was created after subtracting consecutive filtered profile data (80 μm, Gaussian filter), and mean (SD) volume, surface area, and normalised lesion depth was determined within a 1.5 mm circular region of interest (ROI). Volume error (expressed as height variation across ROI surface area) was 0.022 μm (instrument repeatability) and 0.149 μm (method reproducibility), whilst surface area error (expressed as percentage change of the surface area deviation across the entire surface area) was 0.034% (repeatability error) and 0.081% (reproducibility error). Sixty-four natural enamel surfaces taped with polyvinyl-chloride tape leaving 1.5 mm exposed ROI underwent dietary erosion cycling (three 5-min cycles, 0.3% citric acid w/v, pH 3.2) generating artificial erosion lesions. Samples were scanned with NCLP before/after each erosion cycle, scans filtered for microtexture, and after-erosion scans were subtracted from beforeerosion scans. NCLP results show mean (SD) volume, surface area, normalised depth, and 3D stepheight of the eroded area increased significantly after each erosion cycle, with no significant difference in calcium and phosphate release after each cycle. We demonstrate a robust and valid dental model with analysis workflow to measure surface form change in NHE using NCLP, improving understanding of measuring surface form change in complex freeform surfaces.

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“…When looking at the incentive and need for clinical studies of erosive tooth wear, several approaches are relevant. First, it is important to develop effective prevention methods to avoid structural enamel loss, also concluded in a recent study of 3D surface texture characterization [22]. This implies the need for instruments that are sensitive to the initial stages of erosion on natural surfaces in vivo, like SRI OptiPen.…”

Section: Discussionmentioning

confidence: 99%

Analytical strategies for clinical studies on dental erosive wear

Stenhagen

Holme

Tveit³

et al. 2019

BMC Oral Health

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Background There is a need for analytical techniques for measuring Erosive Tooth Wear (ETW) on natural surfaces in clinical studies. The purpose was to investigate the use of two instruments aimed to assess initial to more advanced stages of ETW. Methods Human premolar enamel samples (2x3mm) ( n = 24), were polished flat and mounted in resin cylinders (4 cylinders, 6 samples in each). Part 1: Baseline analyses by White Light Interferometer (WLI), Surface Reflection Intensity (SRI: TableTop and OptiPen) and Surface Hardness (SH). Erosion (1% citric acid (pH 3.6) for 1, 2, 4, 6, 8, 10 min. SRI and SH analyses after every erosion episode and by WLI after 10 min. New indentations were made and enamel loss; was measured by change in indentation depths from toothbrush abrasion (200 g, 60 strokes, 30 s). Another series of 2 × 5 min erosion (totally15 min and 20 min) was analysed with SH and SRI after each erosion, and by WLI (on samples and impressions of samples) after 20 min. Part 2 investigated WLI performance in the interface where initial erosion increases in severity and substance loss occurs. The samples were repolished. Baseline analyses by WLI, SRI (TableTop and OptiPen) and SH. Four cylinders were etched for 1, 2, 4, 8 min respectively and analysed by SRI, SH on samples, and WLI on samples and impressions). Results Part1: SRI decreased from baseline to ~ 6 min etch and increased slightly after abrasion, the two devices correlated well (ICC 0.98 p < 0.001, Spearmans rs 0.91 p < 0.001). SH decreased nearly linearly to 10 min etch, but increased distinctly after abrasion. Mean enamel loss from abrasion alone was 0.2 μm (change in indentation depths). After 10 min etch, it was 0.27 μm (WLI) and after 20 min etch, it was 2.2 μm measured on samples vs 2.4 μm on impressions of samples (7% higher). Part 2: From baseline to 8 min etch; SRI and SH decreased whereas WLI presented increasing etch depths. Conclusions With some adjustments, the use of SRI and WLI in combination seems to be a promising strategy for monitoring ETW in clinical studies.

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Precision of 655 nm Confocal Laser Profilometry for 3D surface texture characterisation of natural human enamel undergoing dietary acid mediated erosive wear

Cited by 18 publications

References 26 publications

The effect of red wine in modifying the salivary pellicle and modulating dental erosion kinetics

The effect of red wine in modifying the salivary pellicle and modulating dental erosion kinetics

Surface form analysis on complex freeform organic structures – measuring erosive wear on human teeth in vitro

Analytical strategies for clinical studies on dental erosive wear

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