Preparation and properties of nano‐hydroxyapatite/PCL‐PEG‐PCL composite membranes for tissue engineering applications

Abstract

Nano-hydroxyapatite (n-HA)/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL, PCEC) composite membranes were prepared by solvent casting and evaporation method. The structure and properties of the membranes were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), water contact angle measurements, in vitro hydrolytic degradation, mechanical test, and cell culture. The effect of n-HA content on physical-chemical properties of the n-HA/PCEC composite membranes was studied. The results showed that the shape and size of micropores of the composite membranes changed with n-HA content increased; the tensile strength decreased with the increase of n-HA content. The osteoblast cell was cultured on the membranes, good cell attachment and growth manner were observed after postseeding for 1 day. MTT assays showed that the n-HA/PCEC membranes had no negative effect on the cell viability and proliferation. These results suggested that the obtained n-HA/PCEC composite membranes in this study might have prospective applications in tissue engineering field.

Interaction of cell culture with composition effects on the mechanical properties of polycaprolactone-hydroxyapatite scaffolds fabricated via selective laser sintering (SLS)

“…For example, Shor et al [20] Previous studies also showed that the addition of HA to PCL does not result in a significant increase in cell numbers. This was structure independent as this observation was made for foams [38], films [39] and surface coatings [40]. The composition has indirect effects such as changes in the localization of the cells due to differences in surface properties.…”

Oxidative stress and apoptosis induced by hydroxyapatite nanoparticles in C6 cells

“…During the past 20 years, there were many studies on physiological functions of HA in vitro and in vivo 33, 34. Owing to its excellent biocompatibility, HA nanoparticles have been widely used as scaffolds in vivo 35, 36. They also have potential applications as the carriers for the controlled delivery of growth factors and drugs 37.…”

“…33,34 Owing to its excellent biocompatibility, HA nanoparticles have been widely used as scaffolds in vivo. 35,36 They also have potential applications as the carriers for the controlled delivery of growth factors and drugs. 37 Previous works have shown that HA nanoparticles could inhibit the proliferation of such cancer cells as liver, colon, stomach, and bone cancer cells.…”

Acceleration of dermal wound healing by using electrospun curcumin‐loaded poly(ε‐caprolactone)‐poly(ethylene glycol)‐poly(ε‐caprolactone) fibrous mats

“…The aim of this study was to develop curcumin‐loaded PCL‐PEG‐PCL fibrous scaffolds and to evaluate their wound healing activity in vivo. In our previous work, we have prepared porous PCL‐PEG‐PCL (PCEC) scaffolds for tissue engineering application. To enlarge the using range and try to find the potential biomaterials as wound dressing, we prepared the curcumin‐loaded PCEC fibrous scaffolds owing to the good biocompatibility of PCEC copolymer.…”