Preparation of leucocyte-and platelet-poor red cell concentrates

Koerner, K.; Stampe, D.

doi:10.1007/bf00320705

Cited by 8 publications

(8 citation statements)

References 8 publications

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“…= 46 • . The two crosses in panel (d) are the λ = 1.3 cm peaks fromKoerner & Sargent (1999).Fig. 4.-HL Tauri maps of the λ = 2.7 mm continuum emission.…”

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confidence: 99%

Unveiling the Circumstellar Envelope and Disk: A Subarcsecond Survey of Circumstellar Structures

Looney

Mundy

Welch

2000

ApJ

421

638

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We present the results of a λ = 2.7 mm continuum interferometric survey of 24 young stellar objects in 11 fields. The target objects range from deeply embedded Class 0 sources to optical T Tauri sources. This is the first sub-arcsecond survey of the λ = 2.7 mm dust continuum emission from young, embedded stellar systems. These multi-array observations, utilizing the high dynamic u,v range of the BIMA array, fully sample spatial scales ranging from 0. ′′ 4 to 60 ′′ , allowing the first consistent comparison of dust emission structures in a variety of systems. The images show a diversity of structure and complexity. The optically visible T Tauri stars (DG Tauri, HL Tauri, GG Tauri, and GM Aurigae) have continuum emission dominated by compact (≤ 1 ′′ ) circumstellar disks. In the cases of HL Tauri and DG Tauri, the disks are resolved. The more embedded near-infrared sources (SVS13 and L1551 IRS5) have continuum emission that is extended and compact. The embedded sources (L1448 IRS3, NGC1333 IRAS2, NGC1333 IRAS4, VLA 1623, and IRAS 16293-2422) have continuum emission dominated by the extended envelope, typically ≥ 85% of the emission at λ = 2.7 mm. In fact, in many of the deeply embedded systems it is difficult to uniquely isolate the disk emission component from the envelope extending inward to AU size scales. Simple estimates of the circumstellar mass in the optical/infrared and embedded systems range from 0.01-0.08 M ⊙ and 0.04-2.88 M ⊙ , respectively. All of the target embedded objects are in multiple systems with separations on scales of ∼ 30 ′′ or less. Based on the system separation, we place the objects into three categories: separate envelope (separation ≥ 6500 AU), common envelope (separation 150-3000 AU), and common disk (separation ≤ 100 AU). These three groups can be linked with fragmentation events during the star formation process: separate envelopes from prompt initial fragmentation and the separate collapse of a loosely condensed cloud, common envelopes from fragmentation of a moderately centrally condensed spherical system, and common disk from fragmentation of a high angular momentum circumstellar disk.

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“…= 46 • . The two crosses in panel (d) are the λ = 1.3 cm peaks fromKoerner & Sargent (1999).Fig. 4.-HL Tauri maps of the λ = 2.7 mm continuum emission.…”

mentioning

confidence: 99%

Unveiling the Circumstellar Envelope and Disk: A Subarcsecond Survey of Circumstellar Structures

Looney

Mundy

Welch

2000

ApJ

421

638

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“…In the present investigations red cells containing few leukocytes and platelets were easily processed in a closed system, and the removal amounts of leukocytes and plate lets from red cells were almost equal to those units pro cessed in open systems [3,8,9].…”

Section: -Imentioning

confidence: 87%

“…The modified warm-centrifuge method by Koerner and Stampe [3] was applied to quadruple bags in which all the procedures were done in a closed system. The third and fourth bags contained, respectively, a sodium phos phate buffer for incubation and a protein-free medium consisting of adenine, phosphate, glucose and 0.9% NaCl solution [4] for red cell preservation.…”

Section: Methodsmentioning

confidence: 99%

Method for Processing Leukocyte- and Platelet-Poor Red Cells in Closed Bags

Shimizu¹,

Ishikawa²,

Hiromi³

et al. 1986

Vox Sanguinis

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If leukocyte- and platelet-poor red cells have been processed and stored in closed bags, their delivery, especially on holidays, will be easy. The modified warm-centrifuge method was applied to a quadruple bag prepared for such purpose. Red cells were processed in the first bag by the centrifugation of whole blood. The supernatant plasma was transferred to the second bag. A phosphate buffer in the third bag was introduced into red cells and diluted red cells were then incubated at 37 °C for 1 h in an inverted position. After centrifugation, the red cell phase below the buffy coat layer was isolated using a Biotest separation apparatus and stored in the fourth bag (containing a preservative with adenine, phosphate, glucose and 0.9% NaCl solution) for 28 days at 4°C. Ninety-eight percent of the leukocyte and 97% of platelets in whole blood unit were removed with a red cell recovery of 86%. Biochemical changes in stored red cells were similar to those of conventional buffy coat-removed red cells suspended in the same preservative, except for rapid decreases in 2,3-diphosphoglycerate levels. Since processing buffy coat-removed red cells containing few leukocytes and platelets with the present method was simple and these units might be stored for over 14 days with minimum cell damage, we suggest that the present method is useful way of processing leukocyteand platelet-poor red cells in blood centers.

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“…On the contrary, the distribution pattern of platelets left in reconstituted RBC was not different from that of whole blood. Unlike previous reports (Schneider and Stutzle 1979;Koerner and Stampe 1982), in which plastic bags were used, no platelets could be removed from RBC. The present experiments provided that our method is useful for removing leukocytes rather than platelets from reconstituted RBC.…”

mentioning

confidence: 91%

“…Recently, Koerner and Stampe (1982) used the buffy coat free RBC as the starting material to prepare them by reconstitution with phosphate buffer, and revealed that the reduction of leukocytes and platelets from reconstituted RBC was equal to frozen red cells or filtered blood (Diepenhorst et al 1972). However, their fate in reconstituted RBC is not clear.…”

mentioning

confidence: 99%