Preparative separation of six antimycin A components from antimycin fermentation broth by high-speed counter-current chromatography

Wang, Jiaoyan; Wen, Yaoming; Chen, Xiuming; Lin, Yuhan; Zhou, Jingming; Xie, You; Wang, Huaiwang; Jiang, Hong; Zheng, Wei

doi:10.1016/j.chroma.2010.07.003

Cited by 13 publications

(8 citation statements)

References 17 publications

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“…Compared with traditional liquid adsorption chromatography in which a solid phase is involved, HPCCC has numerous advantages such as no irreversible adsorption and complete recovery in samples. Because of these advantages, HPCCC is gaining popularity as a purification tool for natural products [9][10][11]13].…”

Section: Introductionmentioning

confidence: 99%

“…Current purification methods include the use of labor intensive and time-consuming columns with a variety of stationary phases and sequential solvent extraction [5][6][7][8]. High performance countercurrent chromatography (HPCCC) is a liquid-liquid chromatographic separation technique used for the isolation of natural compounds [9][10][11][12]. Compared with traditional liquid adsorption chromatography in which a solid phase is involved, HPCCC has numerous advantages such as no irreversible adsorption and complete recovery in samples.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and structural elucidation of indole alkaloids from Geissospermum vellosii by mass spectrometry

Mbeunkui¹,

Grace²,

Lila³

2012

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and structural elucidation of indole alkaloids from Geissospermum vellosii by mass spectrometry

Mbeunkui¹,

Grace²,

Lila³

2012

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

“…CCC is a liquid-liquid partition chromatography that has been widely used for preparative separation of active component from natural product [13], synthetic mixture [14], and fermentation broth [15]. However, only very limited numbers F I G U R E 1 Stereochemical structures of acetyltropic acid enantiomer of studies are available, which are about enantioseparations by CCC [16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Enantioseparation of acetyltropic acid by countercurrent chromatography with sulfobutyl ether‐β‐cyclodextrin as chiral selector

Qiu

Sun

Wang

et al. 2019

J of Separation Science

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Acetyltropic acid is an important synthetic intermediate for preparation of tropane alkaloid derivatives, which can be used as anticholinergic drugs, deliriants, and stimulants. In the present work, acetyltropic acid was successfully enantioseparated by countercurrent chromatography using sulfobutyl ether-β-cyclodextrin as chiral selector. A biphasic solvent system composed of n-butyl acetate/n-hexane/0.1 mol/L citrate buffer at pH = 2.2 containing 0.1 mol/L of sulfobutyl ether-β-cyclodextrin (7:3:10, v/v) was selected, which produced a suitable distribution ratio D S = 1.14, D R = 2.31 and a high enantioseparation factor α = 2.03. Baseline separation was achieved for preparative enantioseparation of 50 mg of racemic acetyltropic acid. A method for chiral analysis of acetyltropic acid by conventional reverse phase liquid chromatography with hydroxylpropyl-β-cyclodextrin as mobile phase additive was established, and formation constants of inclusion complex were determined. It was found that different substituted β-cyclodextrin should be selected for enantioseparation of acetyltropic acid by countercurrent chromatography and reverse phase liquid chromatography. K E Y W O R D Sacetyltropic acid, countercurrent chromatography, cyclodextrins, enantioseparation, liquid chromatography J Sep Sci 2020;43:681-688.

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“…HSCCC, invented by Ito, is a useful method for the preparative separation of natural products as a rapid chromatographic purification by liquid–liquid partitioning in a centrifuged coil of tubing . Being a support‐free liquid–liquid partition method, HSCCC eliminates irreversible adsorption of sample onto the solid support, and allows the direct application of crude extracts and an excellent recovery of the analytes .…”

Section: Introductionmentioning

confidence: 99%

Preparative separation of bioactive compounds from essential oil of Flaveria bidentis (L.) Kuntze using steam distillation extraction and one step high‐speed counter‐current chromatography

Wei

Du²,

Lu³

2012

J of Separation Science

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In order to utilize and control the invasive weed, bioactive compounds from essential oil of Flaveria bidentis (L.) Kuntze were studied. Steam distillation extraction and one step high-speed counter-current chromatography were applied to separate and purify the caryophyllene oxide, 7,11-dimethyl-3-methylene-1,6,10-dodecatriene, and caryophyllene from essential oil of Flaveria bidentis (L.) Kuntze. The two-phase solvent system containing n-hexane/acetonitrile/ethanol (5:4:3, v/v/v) was selected for the one step separation mode according to the partition coefficient values (K) of the target compounds and the separation factor (α). The purity of each isolated fraction after a single high-speed counter-current chromatography run was determined by high performance liquid chromatography. A 3.2 mg of caryophyllene oxide at a purity of 92.6%, 10.4 mg of 7,11-dimethyl-3-methylene-1,6,10-dodecatriene at a purity of 99.1% and 5.7 mg of caryophyllene at a purity of 98.8% were obtained from 200 mg essential oil of Flaveria bidentis (L.) Kuntze. The chemical structures of these components were identified by GC-MS, (1) H-NMR, and (13) C-NMR.

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Preparative separation of six antimycin A components from antimycin fermentation broth by high-speed counter-current chromatography

Cited by 13 publications

References 17 publications

Isolation and structural elucidation of indole alkaloids from Geissospermum vellosii by mass spectrometry

Isolation and structural elucidation of indole alkaloids from Geissospermum vellosii by mass spectrometry

Enantioseparation of acetyltropic acid by countercurrent chromatography with sulfobutyl ether‐β‐cyclodextrin as chiral selector

Preparative separation of bioactive compounds from essential oil of Flaveria bidentis (L.) Kuntze using steam distillation extraction and one step high‐speed counter‐current chromatography

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