Presence of three P types (VP4 serotypes) and two G types (VP7 serotypes) among bovine rotavirus strains

Abstract

Cross neutralization tests with a panel of rotavirus strains representing previously described nine VP7 (G) serotypes revealed that bovine rotavirus strain KK-3, a prototype Japanese bovine serotype 2, belonged to a new serotype (G10), confirming and extending the recent report of Snodgrass et al. [J. Clin. Microbiol. 28: 504-507 (1990)] which showed that hyperimmune serum to the KK-3 strain neutralized the B223 strain, a proposed type strain of G10. Further antigenic characteristics of the KK-3 strain, as well as the 0510 strain (a G6 strain isolated in Japan), were examined in terms of their VP4 (P) specificity. For the characterization of P types, we employed genetic reassortants that possess VP4 gene for UK and VP7 gene for D (G1), VP4 gene for NCDV and VP7 gene for SA11 (G3), or VP4 gene for SA11 and VP7 gene for NCDV (G6) in the plaque reduction neutralization assay with hyperimmune sera against these two Japanese strains and the prototype bovine rotavirus NCDV strain. While the 0510 strain had UK-like P and NCDV-like G types, the KK-3 strain had a distinct set of P and G types. Thus, at least three P types (NCDV-, UK-, and KK-3-like) and two G types (G6 and G10) are present among bovine rotavirus strains.

Identification of four VP4 serological types (P serotypes) of bovine rotavirus using viral reassortants

“…In addition, the antisera to the reassortants reacted with each parental bovine virus (VP4 reaction only) at titres four-to 16-fold lower than to the respective reassortant virus (both VP4 and VP7 reactions), reinforcing the evidence of the relatively minor role of the VP4. Similarly, dominant VP7 reactions with other reassortants have also been described recently (Matsuda et al, 1990).…”

“…It has been recognized that NCDV and UK viruses have distinct VP4s (Kantharidis et al, 1988;Lopez et al, 1991;Matsuda et al, 1990) and that C486 and NCDV gene 4 sequences are similar (Lopez et al, 1991). We have previously detected reaction by RNA-RNA hybridization between a probe prepared from gene 4 of UK virus and 678 Redmond et aL, 1992).…”

“…This study has built on recent work by other authors in typing bovine rotavirus VP4s (Hardy et al, 1991;Matsuda et al, 1990). The serological information will be necessary to analyse and interpret subsequent sequence studies.…”

“…The VP7 or G serotypes are immunodominant, particularly with hyperimmune sera (Hoshino et al, 1984), and a total of 14 G serotypes have been described to date . Bovine rotavirus isolates have been identified as VP7 serotypes G6, G10 and G8, with the first two predominating (Matsuda et al, 1990;Snodgrass et al, 1990;Taniguchi et al, 1991). The genomic basis for serotype variation has been confirmed by sequence analysis of the gene segment encoding VP7 (Estes & Cohen, 1989), and rotavirus serotypes can be predicted by hybridization and/or sequence data (Flores et al, 1989(Flores et al, , 1990Green et al, 1987;Gouvea et al, 1990).…”

“…Sequence data suggest that each mammalian species may be associated with particular VP4 types (Lopez et al, 1991). Bovine rotaviruses have been shown to possess three serologically distinct VP4s: P1 (for strain NCDV), P5 (for UK), and a so-far unclassified P serotype for G10 strains B223 and B-11 (Hardy et al, 1991;Huang et al, 1992;Kantharidis et al, 1988;Matsuda et al, 1990). This paper describes a systematic attempt to type bovine rotavirus VP4s serologically by preparing viral reassortants containing a bovine virus VP4 gene and the G4 human rotavirus ST3 VP7 gene.…”