Prevalence of antibiotic-resistant bacterial strains in wastewater streams: molecular characterization and relative abundance

Fiaz, Marium; Ahmed, Iftikhar; Riaz, Rabaila; Nawaz, Uzma; Arshad, Muhammad

doi:10.1007/s12223-021-00902-z

Cited by 5 publications

(2 citation statements)

References 40 publications

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“…The rapid spread of MDR S. aureus harboring ARGs had become a major problem in veterinary and human medicine worldwide due to the difficulty of treating them. Most infections that lead to severe clinical implications for human health occur via pathogenic potential lineages and are classified as high-risk microbiological hazards in Hazard Analysis Critical Control Point (HACCP) (Ali et al 2021a;Dehbashi et al 2021;Fiaz et al 2021;Rodríguez-Lázaro et al 2017;Saima et al 2020). This present study is the first investigation to evaluate the spread of AMR and ARGs of S. aureus in Rubing and Rushan cheese in Yunnan province, China, through the integration of phenotypic approach and in silico genome-based methods using publicly available databases.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and antimicrobial resistance profiling of Staphylococcus aureus isolated from traditional cheese in Yunnan, China

et al. 2021

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The prevalence of staphylococcal infection and the emergence of multidrug resistance of Staphylococcus aureus (S. aureus) are major concerns in food safety and public health. This study aimed to investigate the prevalence of S. aureus isolated from traditional Chinese Rubing and Rushan cheese, antimicrobial resistance profiles, genomic characteristics, and predict antimicrobial resistance genes (ARGs). From 124 samples, 18 of 62 (29.03%) of Rubing and 5 of 62 (8.06%) of Rushan cheese were confirmed to be S. aureus positive by standard culture-based methods. Twenty-three coagulase-positive staphylococci isolates were grouped into 16 clusters by pulsed-field gel electrophoresis and subjected to routine susceptibility testing to 12 antibiotics. Those isolates exhibited high resistance to penicillin (100%), erythromycin, trimethoprim-sulphamethoxazole (34.78%), oxacillin, clindamycin, and cefoxitin (21.74%). Multidrug-resistant (MDR) S. aureus was found in 34.78% (8 of 23) of isolates. Further, S. aureus strain DC.RB_015 isolated from Rubing cheese, recognized as the most resistant to six antibiotics, was selected for whole-genome sequencing (WGS), continued with in silico approaches. S. aureus DC.RB_015 had a single chromosome size of 2,794,578 bp and a plasmid size of 22,961 bp. The strain harbored 18 predicted ARGs, including eight efflux pump genes (mepA, tet(K), arlR, arlS, norA, mgrA, tet(38), LmrS), one peptidoglycan biosynthesis gene (bacA), two β-lactams resistance genes (mecA, blaZ), and seven genes conferring other antimicrobial resistance (APH(3′)-IIIa, aad(6), ErmB, mecR1, GlpT, murA). The results of this study expand the knowledge of S. aureus strain DC.RB_015, increase food safety awareness, and will be helpful in establishing therapeutic therapy.

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Section: Discussionmentioning

confidence: 99%

Prevalence and antimicrobial resistance profiling of Staphylococcus aureus isolated from traditional cheese in Yunnan, China

et al. 2021

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“…The collective effect of different mechanisms of resistance leads to the development of multidrug-resistant bacterial pathogens, which may result in life-threatening infections that are extremely hard to treat. Nowadays, the rise of multi-drug resistant bacterial pathogens is of global public health concern, particularly in hospital set-tings (1). One of the most important bacteria is multidrugresistant Klebsiella pneumoniae (2).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of a Wastewater Siphoviridae Bacteriophage Targeting Multidrug-resistant Klebsiella pneumoniae

Qadri

Harakeh

Alamri

et al. 2021

Jundishapur J Microbiol

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Background: Based on the WHO, multidrug-resistant Klebsiella pneumoniae is a priority pathogen that causes opportunistic infections and is widely spread in the environment. Phage therapy is considered a natural, safe, and very efficient alternative to treat difficult-to-treat infections. Objectives: This study aimed to isolate highly virulent, lytic bacteriophages and evaluate their efficacy for lysing multidrug-resistant K. pneumoniae. Methods: Municipal wastewater samples were collected and filtered using 0.22 µm syringe filters and cultivated with log-phase cultures of K. pneumoniae using enrichment media. After 48 h of incubation, the cultures were centrifuged, and the resultant supernatant was filtered (0.22 µm). The detection of the phage was done using the spot assay with K. pneumoniae as the host. One-step growth kinetics and bacterial reduction tests were conducted to assess the growth kinetics of the isolated phage. The stability of the isolated phage was characterized by subjecting it to various temperature and pH conditions. The chemical stability of the K. pneumoniae phage was determined by exposing it to various organic compounds. A panel of 20 bacterial strains was tested using the spot assay, as well as double agar overlying assay, to determine the host range of the isolated phage. Results: Out of 40 wastewater samples tested, only one sample was tested positive for the K. pneumoniae phage (2.5%) that was lytic against the host strain. The K. pneumoniae phage had a latent period of 15 min and a burst size of 100 virions per infected cell. It was most stable at 37°C and pH range of 6.0 to 10.0. Chemically, the K. pneumoniae phage was resistant to 10% chloroform treatment. Transmission electron micrograph indicated that the K. pneumoniae phage belonged to the order Caudovirales, family Siphoviridae, morphotype B1. Conclusions: Most of the characteristic features of the K. pneumoniae phage indicated the potential of this phage to be used in phage therapy. Hence, a comprehensive study is highly recommended to characterize the K. pneumoniae phage genome, detect its molecular interactions with the host cell, and determine its lytic activity in combination with other phages, which may lead to the efficient utilization of this phage in phage therapy against K. pneumoniae infections.

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Metallic nanoparticles photodegraded antibiotics and co-application improved wheat growth and nutritional quality through stress alleviation

et al. 2023

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Prevalence of antibiotic-resistant bacterial strains in wastewater streams: molecular characterization and relative abundance

Cited by 5 publications

References 40 publications

Prevalence and antimicrobial resistance profiling of Staphylococcus aureus isolated from traditional cheese in Yunnan, China

Prevalence and antimicrobial resistance profiling of Staphylococcus aureus isolated from traditional cheese in Yunnan, China

Isolation and Identification of a Wastewater Siphoviridae Bacteriophage Targeting Multidrug-resistant Klebsiella pneumoniae

Metallic nanoparticles photodegraded antibiotics and co-application improved wheat growth and nutritional quality through stress alleviation

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