Prevention of Aberrant Crypt Foci Formation by Dietary Maize and Yeast Cerebrosides in 1,2-Dimethyihydrazine-treated Mice

Abstract

Sphingolipids are each comprised of a sphingoid base backbone, amide-linked fatty acids (normal and 2hydroxy fatty acids) and a polar headgroup, such as phosphocholine or hexose. Sphingolipids are frequently to be found in eukaryotic organisms, with mammalian sphingolipid trans-4-sphingenine (sphingosine) being most prevalent. Others such as sphinganine (dihydrosphingosine) and 4-hydroxysphinganine (phytosphingosine) frequently occur in small amounts. Plant and fungus sphingolipids display a diversity of sphingoid base structures, such as those of trans-8-sphingenine, cis-8-sphingenine, trans-4, trans-8-sphingadienine, trans-4, cis-8-sphingadienine, 4-hydroxy-trans-8-sphingenine, 4-hydroxy-cis-8-sphingenine and 9-methyl-45

Effects of Dietary Plant-origin Glucosylceramide on Colon Cytokine Contents in DMH-treated Mice

“…DMH administration caused injury to the crypts, but treatment with dietary GlcCer maintained the colon in the normal state. The results obtained were essentially the same as those of our previous studies 4 . A cytokine array was used to determine the effects of dietary GlcCer on inflammation-related cytokine production in DMH-treated mice for 7 weeks.…”

“…ACF on large intestinal crypts were identified and quantified as described previously 4 . The large intestine was excised from the mice under pentobarbitone anesthesia, and the portion from the cecum to the vent was separated and rinsed in cold saline.…”

“…In our previous study, we investigated the effects of dietary GlcCer on aberrant crypt foci ACF formation in the large intestines of mice treated with 1,2-dimethylhydrazine DMH 4 . We found that DMH administration caused injury to the crypts; however, concurrent administration of dietary GlcCer was associated with maintenance of the colon in the normal state.…”

Effects of Dietary Plant-Origin Glucosylceramide on Bowel Inflammation in DSS-Treated Mice

“…For isolation of GlcCer, the preparation was dissolved in chloroform, applied to a silica gel column, and purified by preparative thin layer chromatography TLC , as described previously 2,15 . The purity of GlcCer 80 was determined by HPLC, as described previously 15 .…”

“…In our previous study, we investigated the effects of dietary GlcCer on aberrant crypt foci formation in the large intestines of mice treated with 1,2-dimethylhydrazine DMH 15 . In this study, dietary GlcCer suppressed damage of the large intestines by DMH treatment.…”