Prevention of Renal Scarring in Acute Pyelonephritis by Probiotic Therapy: an Experimental Study

Abstract

We evaluated the protective effects of probiotic administration as a prophylaxis treatment and immediately after fever onset in increasing the immune response and decreasing the renal scarring in a rat model of acute pyelonephritis. Twenty-four rats were apportioned to three groups. In GI (n = 8), the rats were injected with direct inoculation of Escherichia coli into the right kidney. In GII (n = 8), the rats received a probiotic regimen 1 month before E. coli injection and the probiotic regimen was continued for the next 2 months. In GIII (n = 8), the probiotic regimen was started just after E. coli injection and was continued for 2 months. Technetium-99m-DMSA renal scan, histopathological evaluations, concentrations of CA19-9, IgA, blood urea nitrogen (BUN), and creatinine were assessed 1 and 2 months post-injection. It took an average of 4.2 ± 1.1 h between the injection and onset of fever in GI and GII. In GIII, this period was longer (7.5 ± 1.4). Probiotic administration resulted in reduction of interstitial fibrosis and tubular and glomerular atrophy in GII in all follow-ups. Technetium-99m-DMSA renal scan showed that the right kidney reached near the normal cortical integrity (47%) in GII compared to GI (32%) after 2 months of injection. However, the renal integrity did not improve significantly in GIII (41%). In GII, CA19-9 was lower (p < 0.05), while the levels of serum and fecal IgA were higher (p < 0.05). Administration of the probiotic regimen in the rat model may decrease renal damage in pyelonephritis. In spite of better results in the prophylactic group compared to the treatment group, no strong evidence was found to prove the advantage of its prophylactic application over the treatment administration.

Probiotics in septic acute kidney injury, a double blind, randomized control trial

“…However, previous experimental models have been encouraging. In mice induced to exhibit pyelonephritis with E. coli injection, it was shown that the administration of Lactobacillus acidophilus and Bifidobacterium before and after sepsis significantly improved renal function and attenuated inflammation and renal fibrosis [ 40 ]. Similarly, administration of SCFAs improved renal function after AKI, which was an effect mediated by the decrease in sCr and urea levels, and it also improved the percentage of necrosis seen in kidney biopsies.…”

Acute Kidney Injury and Intestinal Dysbiosis

“…In another experimental model, AKI was induced in rats by urosepsis via E. coli inoculation. In this model, the administration of probiotics ( Lactobacillus acidophilus and Bifidobacterium ) one month before and two months after renal insult significantly attenuated the histological evidence of kidney injury ( 34 ).…”