Preventive Effect of Aspirin Eugenol Ester on Thrombosis in κ-Carrageenan-Induced Rat Tail Thrombosis Model

Abstract

Based on the prodrug principle, aspirin eugenol ester (AEE) was synthesized, which can reduce the side effects of aspirin and eugenol. As a good candidate for new antithrombotic and anti-inflammatory medicine, it is essential to evaluate its preventive effect on thrombosis. Preventive effect of AEE was investigated in κ-carrageenan-induced rat tail thrombosis model. AEE suspension liquids were prepared in 0.5% sodium carboxymethyl cellulose (CMC-Na). AEE was administrated at the dosage of 18, 36 and 72 mg/kg. Aspirin (20 mg/kg), eugenol (18 mg/kg) and 0.5% CMC-Na (30 mg/kg) were used as control drug. In order to compare the effects between AEE and its precursor, integration of aspirin and eugenol group (molar ratio 1:1) was also designed in the experiment. After drugs were administrated intragastrically for seven days, each rat was injected intraperitoneally with 20 mg/kg BW κ-carrageen dissolved in physiological saline to induce thrombosis. The length of tail-thrombosis was measured at 24 and 48 hours. The blank group just was given physiological saline for seven days without κ-carrageenan administrated. The results indicated that AEE significantly not only reduced the average length of thrombus, PT values and FIB concentration, but also reduced the red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT) and platelet (PLT). The effects of AEE on platelet aggregation and anticoagulant in vitro showed that AEE could inhibit adenosine diphosphate (ADP)-induced platelet aggregation as dose-dependence but no notable effect on blood clotting. From these results, it was concluded that AEE possessed positive effect on thrombosis prevention in vivo through the reduction of FIB, PLT, inhibition of platelet aggregation and the change of TT and PT values.

Lowering effects of aspirin eugenol ester on blood lipids in rats with high fat diet

“…The positive effects of AEE on the symptoms of inflammation, fever, pain and thrombosis had been confirmed in animal disease model [9, 11, 13]. The blood chemistry results in 15-day oral dose toxicity study showed AEE could significantly reduce the values of TG and TCH both in male and female rat, which indicated that this compound was potential for curing hyperlipidemia [11].…”

“…Moreover, the effects of AEE had been evaluated in animal model. The results showed that AEE had positive effects on antithrombosis, anti-inflammatory, analgesia and antipyretic [9, 11, 13]. …”

Photocatalytic Performance and Degradation Mechanism of Aspirin by TiO2 through Response Surface Methodology

“…Aspirin, known as acetylsalicylic acid (ASA), is commonly used as a classic non-steroidal anti-inflammatory drug (NSAID) to relieve minor aches and treat inflammation and cardiovascular diseases [3,4]. However, this type of pharmaceutical has been detected in the aquatic environment at trace concentrations (ng to µg/L), which can prolong exposure to environmentally relevant concentration and considerably increase the toxicity considerably, resulting in negative consequences for aquatic life and human beings [5][6][7].…”

Photocatalytic degradation of aspirin from aqueous solutions using the UV/ZnO process: modelling, analysis and optimization by response surface methodology (RSM)

“…ASA has an inhibitory effect on platelets in blood. This antiplatelet effect prevents the formation of blood clots in blood vessels, especially in people with atherosclerosis (blood vessels stenosis) [7,8]. The increased use of ASA causes increased ASA concentration in wastewater as well as in surface and underground water and thus affects aquatic ecosystems [9].…”