Probing DNA-stabilized fluorescent silver nanocluster spectral heterogeneity by time-correlated single photon counting

Carro‐Temboury, Miguel R.; Paolucci, Valentina; Hooley, E.; Latterini, Loredana; Vosch, Tom

doi:10.1039/c5an02011e

Cited by 34 publications

(38 citation statements)

References 40 publications

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“…7). In comparison, a number of other AgNCs have reported extinction coefficients exceeding several hundred thousand M −1 cm −1 in combination with nanosecond decay times1029.…”

Section: Resultsmentioning

confidence: 93%

Ultrafast coherence transfer in DNA-templated silver nanoclusters

et al. 2017

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DNA-templated silver nanoclusters of a few tens of atoms or less have come into prominence over the last several years due to very strong absorption and efficient emission. Applications in microscopy and sensing have already been realized, however little is known about the excited-state structure and dynamics in these clusters. Here we report on a multidimensional spectroscopy investigation of the energy-level structure and the early-time relaxation cascade, which eventually results in the population of an emitting state. We find that the ultrafast intramolecular relaxation is strongly coupled to a specific vibrational mode, resulting in the concerted transfer of population and coherence between excited states on a sub-100 fs timescale.

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“…7). In comparison, a number of other AgNCs have reported extinction coefficients exceeding several hundred thousand M −1 cm −1 in combination with nanosecond decay times1029.…”

Section: Resultsmentioning

confidence: 93%

Ultrafast coherence transfer in DNA-templated silver nanoclusters

et al. 2017

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“…Silver nanoparticles (AgNPs) have garnered much attention due to the wide range of applications in catalysis 1 , membrane bioreactors 2 , DNA sequencing 3 and cancer treatment 4 . Several physical and chemical strategies have been employed for the production of nanoparticles (NPs) 5 .…”

Section: Introductionmentioning

confidence: 99%

Insight into eco-friendly fabrication of silver nanoparticles by Pseudomonas aeruginosa and its potential impacts

Ali

Jamil

et al. 2017

Journal of Environmental Chemical Engineering

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Abstract. Although green synthesis of nanoparticles (NPs) has replaced conventio na l physicochemical methods owing to eco-friendly and cost effective nature but molecular mechanism is not known completely. Elucidation of the mechanism is needed to enhance the production of control size synthesis and for understanding the biomineralization process. Here we report the facile, extracellular biosynthesis of silver nanoparticles (AgNPs) by Pseudomonas aeruginosa JP1 through nitrate reductase mediated mechanism. AgNO3 was reduced to AgNPs by cell filtrate exposure. UV-visible spectrum of the reaction mixture depicted reduction of ionic silver (Ag + ) to atomic silver (Ag 0 ) by a progressive upsurge in surface plasmon resonance (SPR) band range 435-450 nm. X-ray diffraction analysis showed the 2θ values at 38.08°, 44.52°, 64.42° and 77.44° confirming the crystalline nature and mean diameter [6.5-27.88nm (Ave = 13.44 nm)] of AgNPs. Transmission electron microscopy analysis demonstrated the spherical AgNPs with size range 5-45 nm. Stabilizing proteins and rhamnolipids were recognized by Fourier transform infrared spectroscopy. Nitrate reductase was purified and characterized (molecular weight 65 kDa and specific activity = 5.6 U/mg). To probe the plausible mechanism purified enzyme was retreated with AgNO3. Characteristic SPR bands range (435-450 nm) and Particle-induced x-ray emission results also confirmed the synthesis of AgNPs (59679.5 ppm) in solution. These results demonstrated that, nitrate reductase as a principal reducing agent in the mechanistic pathway of AgNPs synthesis, which leads to the understanding of metal transformation and biomineralization processes for controlling the biogeochemical cycles of silver and other heavy metals.

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“…Additionally, the emission maxima shifted slightly during the course of the experiment. The latter is not uncommon and has also been reported for AgNCs stabilized in DNA …”

Section: Resultsmentioning

confidence: 53%

Peptide‐Stabilized, Fluorescent Silver Nanoclusters: Solid‐Phase Synthesis and Screening

Gregersen

Vosch

Jensen

2016

Chemistry A European J

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Few-atom silver nanoclusters (AgNCs) can exhibit strong fluorescence; however, they require ligands to prevent aggregation into larger nanoparticles. Fluorescent AgNCs in biopolymer scaffolds have so far mainly been synthesized in solution, and peptides have only found limited use compared to DNA. Herein, we demonstrate how solid-phase methods can increase throughput dramatically in peptide ligand screening and in initial evaluation of fluorescence intensity and chemical stability of peptide-stabilized AgNCs (P-AgNCs). 9-Fluorenylmethyloxycarbonyl (Fmoc) solid-phase peptide synthesis on a hydroxymethyl-benzoic acid (HMBA) polyethylene glycol polyacrylamide copolymer (PEGA) resin enabled on-resin screening and evaluation of a peptide library, leading to identification of novel peptide-stabilized, fluorescent AgNCs. Using systematic amino acid substitutions, we synthesized and screened a 144-member library. This allowed us to evaluate the effect of length, charge, and Cys content in peptides used as ligands for AgNC stabilization. The results of this study will enable future spectroscopic studies of these peptide-stabilized AgNCs for bioimaging and other applications.

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Probing DNA-stabilized fluorescent silver nanocluster spectral heterogeneity by time-correlated single photon counting

Cited by 34 publications

References 40 publications

Ultrafast coherence transfer in DNA-templated silver nanoclusters

Ultrafast coherence transfer in DNA-templated silver nanoclusters

Insight into eco-friendly fabrication of silver nanoparticles by Pseudomonas aeruginosa and its potential impacts

Peptide‐Stabilized, Fluorescent Silver Nanoclusters: Solid‐Phase Synthesis and Screening

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