Probing Nanoscale Interactions of Antimicrobial Zinc Oxide Quantum Dots on Bacterial and Fungal Cell Surfaces

Gangadoo, Sheeana; Xu, Chenglong; Cozzolino, Daniel; Latham, Kay; Gaspera, Enrico Della; Chapman, James; Truong, Vi Khanh

doi:10.1002/admi.202101484

Cited by 13 publications

(12 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CLSM and SEM microscopies were employed to visualize the antifungal effect of lipid particles in acidic versus neutral pH after 24 h (Figure 5). 43,44 Untreated fungal cells exhibited high intensity of the green fluorescence (changed from the initial blue color obtained from CLSM for enhanced visualization) showing high chitin concentration and an indication of healthy cells. The chitin is an important component of the fungal cell wall and gives its rigid structure.…”

Section: Antifungal Studies 341 Minimum Inhibitory Concentrationmentioning

confidence: 99%

Application of Fluconazole-Loaded pH-Sensitive Lipid Nanoparticles for Enhanced Antifungal Therapy

Rajesh

Gangadoo

Nguyen

et al. 2022

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Cryptococcus neoformans is a yeast-like fungus that can cause the life-threatening disease cryptococcal meningitis. Numerous reports have shown increased resistance of this fungus against antifungal treatments, such as fluconazole (Fluc), contributing to an 80% global mortality rate. This work presents a novel approach to improve the delivery of the antifungal agent Fluc and increase the drug's targetability and availability at the infection site. Exploiting the acidic environment surrounding a C. neoformans infected site, we have developed pHsensitive lipid nanoparticles (LNP) encapsulating Fluc to inhibit the growth of resistant C. neoformans. The LNP-Fluc delivery system consists of a neutral lipid monoolein (MO) and a novel synthetic ionizable lipid 2-morpholinoethyl oleate (O2ME). At neutral pH, because of the presence of O2ME, the nanoparticles are neutral and exhibit a liquid crystalline hexagonal nanostructure (hexosomes). At an acidic pH, they are positively charged with a cubic nanostructure (cubosomes), which facilitates the interaction with the negatively charged fungal cell wall. This interaction results in the MIC50 and MIC90 values of the LNP-Fluc being significantly lower than that of the free-Fluc control. Confocal laser scanning microscopy and scanning electron microscopy further support the MIC values, showing fungal cells exposed to LNP-Fluc at acidic pH were heavily distorted, demonstrating efflux of cytoplasmic molecules. In contrast, fungal cells exposed to Fluc alone showed cell walls mostly intact. This current study represents a significant advancement in delivering targeted antifungal therapy to combat fungal antimicrobial resistance.

show abstract

Section: Antifungal Studies 341 Minimum Inhibitory Concentrationmentioning

confidence: 99%

Application of Fluconazole-Loaded pH-Sensitive Lipid Nanoparticles for Enhanced Antifungal Therapy

Rajesh

Gangadoo

Nguyen

et al. 2022

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…Other environmentally friendly quantum dots such as ZnO quantum dots and silicon quantum dots are also widely used in the field of LSCs. , Among them, silica quantum dots are ideal emitters for LSCs due to their large reserves on earth, nontoxicity, excellent ability to inhibit reabsorption, and excellent compatibility with polymers. − ZnO quantum dots, on the other hand, are easy to prepare in solution, inexpensive, with good UV absorption and high stability, and show unique potential. − …”

Section: Single-layer Lscsmentioning

confidence: 99%

“… 81 − 83 ZnO quantum dots, on the other hand, are easy to prepare in solution, inexpensive, with good UV absorption and high stability, and show unique potential. 84 − 86 …”

Section: Single-layer Lscsmentioning

confidence: 99%

Achieving High-Efficiency Large-Area Luminescent Solar Concentrators

Cao

Zhao

Gong

2023

JACS Au

View full text Add to dashboard Cite

Luminescent solar concentrators (LSCs) are semitransparent windows that are able to generate electricity from sunlight absorption. LSCs have shown huge promise for realizing building-integrated photovoltaics (BIPV). Unfortunately, to date, the power conversion efficiency (PCE) of LSCs is still very low which dramatically hampers their practical applications. In this Perspective, We summarize and review the latest developments of LSCs by looking at different structures. Among others, we focus more on the next developments in the field of LSCs, i.e., the possibility of high PCE, large area, mass production, and durability needed for future industrial development. We hope to promote the application of uniform testing standards and to draw attention to industrial development, toxicity, and durability. Then, we will provide a critical assessment of the field of LSCs. Finally, the challenge and solution will be discussed.

show abstract

Section: Applications Of Zinc Oxide Colloidal Nanocrystalsmentioning

confidence: 99%

Colloidal Approaches to Zinc Oxide Nanocrystals

et al. 2022

Self Cite

View full text Add to dashboard Cite

Zinc oxide is an extensively studied semiconductor with a wide band gap in the near-UV. Its many interesting properties have found use in optics, electronics, catalysis, sensing, as well as biomedicine and microbiology. In the nanoscale regime the functional properties of ZnO can be precisely tuned by manipulating its size, shape, chemical composition (doping), and surface states. In this review, we focus on the colloidal synthesis of ZnO nanocrystals (NCs) and provide a critical analysis of the synthetic methods currently available for preparing ZnO colloids. First, we outline key thermodynamic considerations for the nucleation and growth of colloidal nanoparticles, including an analysis of different reaction methodologies and of the role of dopant ions on nanoparticle formation. We then comprehensively review and discuss the literature on ZnO NC systems, including reactions in polar solvents that traditionally occur at low temperatures upon addition of a base, and high temperature reactions in organic, nonpolar solvents. A specific section is dedicated to doped NCs, highlighting both synthetic aspects and structure−property relationships. The versatility of these methods to achieve morphological and compositional control in ZnO is explicated. We then showcase some of the key applications of ZnO NCs, both as suspended colloids and as deposited coatings on supporting substrates. Finally, a critical analysis of the current state of the art for ZnO colloidal NCs is presented along with existing challenges and future directions for the field.

show abstract

Probing Nanoscale Interactions of Antimicrobial Zinc Oxide Quantum Dots on Bacterial and Fungal Cell Surfaces

Cited by 13 publications

References 76 publications

Application of Fluconazole-Loaded pH-Sensitive Lipid Nanoparticles for Enhanced Antifungal Therapy

Application of Fluconazole-Loaded pH-Sensitive Lipid Nanoparticles for Enhanced Antifungal Therapy

Achieving High-Efficiency Large-Area Luminescent Solar Concentrators

Colloidal Approaches to Zinc Oxide Nanocrystals

Contact Info

Product

Resources

About