Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract

Abstract

One strain of Lactobacillus salivarius, two strains of Lactobacillus reuteri and Lactobacillus amylovorus, and two strains of Bifidobacterium thermacidophilum with antagonistic effect against Clostridium perfringens were isolated from porcine gastrointestinal tract. Isolates were assayed for their ability to survive in synthetic gastric juice at pH 2.5 and were examined for their ability to grow on agar plate containing porcine bile extract. There was a large variation in the survival of the isolates in gastric juice and growth in the medium containing 0.3% (w/v) bile. L. salivarius G11 and L. amylovorus S6 adhered to the HT-29 epithelial cell line. Cell-free supernatant of L. amylovorus S6 showed higher antagonistic activity as effective as the antibiotics such as neomycin, chlortetracycline, and oxytetracycline against bacterial pathogens including C. perfringens, Salmonella typhimurium, Staphylococcus aureus, Vibrio cholerae, Edwardsiella tarda, and Aeromonas salmonicida subsp. salmonicida.

Alteration of the Ileal Microbiota of Weanling Piglets by the Growth-Promoting Antibiotic Chlortetracycline

“…L. johnsonii, the predominant lactic acid bacterium in control pigs, is autochthonous to the porcine GI tract (27). Lactobacillus bacteria are known to produce an array of bacteriocins, and many isolates have shown promise as probiotics (22,26,37). Various strains of L. johnsonii and L. amylovorus display probiotic activity and also produce antibacterial peptides (33).…”

Oral administration of Lactobacillus plantarum strain Lq80 to weaning piglets stimulates the growth of indigenous lactobacilli to modify the lactobacillal population

“…Minor species such as L. salivarius and L. mucosae not detected under control condition were isolated from piglets after the administration of strain Lq80. Interestingly, the members of these minor lactobacilli have been known to inhibit pathogens (Kim et al, 2007;Robredo and Torres, 2000). Since each species has its own functions, an increase in the diversity of the lactobacillal population may lead to a system less vulnerable to pathogenic penetration.…”

Anticarcinogenecity of microbiota and probiotics in breast cancer

“…[99,100] Probiotic strains including B. animalis, L. acidophilus, Bifidobacterium infantis, Lactobacillus paracasei, Bifidobacterium bifidum were observed to reduce cancer cell growth in MCF7 cells. [101,102] L. helveticus R389 was involved in increased immunity booster; cytokines (including IL-10 and IL-4) and decreased growth of tumor cells when receive 4T1 mouse mammary adenocarcinoma cells injection subcutaneous to murine model (BALB/c). [77,102] Enterococcus lactis IW5 produce metabolites having cytotoxic potential on various cancer cells.…”

“…[101,102] L. helveticus R389 was involved in increased immunity booster; cytokines (including IL-10 and IL-4) and decreased growth of tumor cells when receive 4T1 mouse mammary adenocarcinoma cells injection subcutaneous to murine model (BALB/c). [77,102] Enterococcus lactis IW5 produce metabolites having cytotoxic potential on various cancer cells. These metabolites after 24 h of incubation reduce the growth of breast cancer cells.…”