Probiotics Prevents Sensitization to Oral Antigen and Subsequent Increases in Intestinal Tight Junction Permeability in Juvenile–Young Adult Rats

Tulyeu, Janyerkye; Kumagai, Hideki; Jimbo, Eriko F.; Watanabe, Shinya; Yokoyama, Koji; Cui, Longzhu; Osaka, Hitoshi; Mieno, Makiko; Yamagata, Takanori

doi:10.3390/microorganisms7100463

Cited by 28 publications

(24 citation statements)

References 51 publications

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“…In animal models of FA, oral sensitization to ovalbumin (OVA) induced an increase in the lactulose/mannitol (La/Ma) ratio (indicative of increased intestinal permeability) in excreted urine after two weeks of allergen intake, followed by an increase in serum anti-OVA IgE and a downregulation of ZO-1, claudin-2, claudin-8, and claudin-15 gene expression but upregulation of claudin-9 [ 106 ]. Similarly, rats with FA to OVA demonstrated downregulation of ZO-1, occludin, and claudins 1, 3, 5, 7, 8, 9, and 15 in intestinal tissue; which was accompanied by a widening and shortening of tight and adherent junctions, an increase in excreted La/Ma ratio, and a relocation of some TJ proteins in enterocytes [ 108 ]. The increase in intestinal permeability has been linked to intestinal mastocytosis and IL-9 overexpression ( Figure 4 ), which are associated with a predisposition to clinical manifestations, such as diarrhea, in the absence of allergen sensitization [ 104 ].…”

Section: Intestinal Epithelial Barrier Disordersmentioning

confidence: 99%

Bioactive Compounds in Food as a Current Therapeutic Approach to Maintain a Healthy Intestinal Epithelium

et al. 2021

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The intestinal epithelium serves as an effective barrier against the external environment, hampering the passage of potentially harmful substances (such as pathogenic microbes) that could trigger an exacerbated host immune response. The integrity of this barrier is thus essential for the maintenance of proper intestinal homeostasis and efficient protective reactions against chemical and microbial challenges. The principal consequence of intestinal barrier defects is an increase in intestinal permeability, which leads to an increased influx of luminal stressors, such as pathogens, toxins, and allergens, which in turn trigger inflammation and immune response. The fine and fragile balance of intestinal homeostasis can be altered by multiple factors that regulate barrier function, many of which are poorly understood. This review will address the role of gut microbiota as well as food supplements (such as probiotics, prebiotics, and synbiotics) in modulating gut health and regulating intestinal barrier function. In particular, we will focus on three human pathologies: inflammatory bowel disease, irritable bowel syndrome, and food allergy.

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Section: Intestinal Epithelial Barrier Disordersmentioning

confidence: 99%

Bioactive Compounds in Food as a Current Therapeutic Approach to Maintain a Healthy Intestinal Epithelium

et al. 2021

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“…Increased permeability is thought to be associated with intestinal pathogenesis ( 17 , 18 ). The intestinal mucus in juvenile animals is immature, which is easy to be infected by intestinal bacteria.…”

Section: Resultsmentioning

confidence: 99%

Cinnamaldehyde Promotes the Intestinal Barrier Functions and Reshapes Gut Microbiome in Early Weaned Rats

Mao

Lü³

et al. 2021

Front. Nutr.

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Cinnamaldehyde is an aromatic aldehyde isolated from the essential oil of cinnamon. It has been proved to possess various bioactivities such as anti-inflammation, anti-bacteria and antihypertensive. Nevertheless, early weaning could lead to intestinal stress, causing a range of intestinal health problems. The aim of this study is to explore the effects of cinnamaldehyde on gut barrier integrity, inflammatory responses, and intestinal microbiome of early weaned rats. In this study, treatment with cinnamaldehyde (100 or 200 mg/kg bodyweight/day) for 2 weeks significantly promoted the production of mucins in the colonic epithelial tissue of rats. Cinnamaldehyde supplementation significantly upregulated the expression of Muc2, TFF3 and the tight junction proteins (ZO-1, claudin-1, and occludin). Hematoxylin and eosin staining results showed that colonic histopathological changes were recovered by cinnamaldehyde supplementation. The mRNA expression of IL-6 and TNF-α were significantly decreased in the cinnamaldehyde groups while the TNF-α protein levels were significantly decreased in the two cinnamaldehyde groups. Cinnamaldehyde treatment obviously attenuated the activation of NF-κB signaling pathway in rat colonic tissue and suppressed the production of inflammatory cytokines. Furthermore, cinnamaldehyde supplementation remodeled the gut microbiome structure, at the genus level, Akkermansia, Bacteroides, Clostridium III, Psychrobacter, Intestinimonas were increased, whereas those of Ruminococcus, Escherichia/Shigella were obviously decreased in the cinnamaldehyde treated groups. These findings indicated that cinnamaldehyde could effectively enhance intestinal barrier integrity, ameliorate inflammatory responses and remodel gut microbiome in early weaned rats.

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“…Few studies have examined the effect of anti-allergic probiotics on intestinal barrier function. Among them, a mixture of Lactobacillus strains that reduces allergen sensitization in Bet v1 pollen-sensitized mice upregulates the expression of occludin and the TJ molecule ZO-1, increases total intestinal and serum IgA levels, and improves the function of the gut epithelial barrier [48]; Clostridium butyricum and Lactobacillus reuteri treatments which downregulate allergic immune response in a rat model of food sensitization decrease intestinal permeability and increase tight junction expression [49]. Additionally, it has been demonstrated that probiotic lactobacilli ( L. rhamnosus 19070-2 and L. reuteri DSM 12246) reduce small intestinal permeability in children with atopic dermatitis [50].…”

Section: Discussionmentioning

confidence: 99%

Lactococcus lactis NZ9000 Prevents Asthmatic Airway Inflammation and Remodelling in Rats through the Improvement of Intestinal Barrier Function and Systemic TGF-β Production

Cervantes‐García

Jiménez

Rivas‐Santiago

et al. 2020

Int Arch Allergy Immunol

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Introduction: The use of probiotics has been broadly popularized due to positive effects in the attenuation of aberrant immune responses such as asthma. Allergic asthma is a chronic respiratory disease characterized by airway inflammation and remodelling. Objective: This study was aimed to evaluate the effect of oral administration of Lactococcus lactis NZ9000 on asthmatic airway inflammation and lung tissue remodelling in rats and its relation to the maintenance of an adequate intestinal barrier. Methods: Wistar rats were ovalbumin (OVA) sensitized and challenged and orally treated with L. lactis. Lung inflammatory infiltrates and cytokines were measured, and remodelling was evaluated. Serum OVA-specific immunoglobulin (Ig) E levels were assessed. We also evaluated changes on intestinal environment and on systemic immune response. Results: L. lactis diminished the infiltration of proinflammatory leucocytes, mainly eosinophils, in the bronchoalveolar compartment, decreased lung IL-4 and IL-5 expression, and reduced the level of serum allergen-specific IgE. Furthermore, L. lactis prevented eosinophil influx, collagen deposition, and goblet cell hyperplasia in lung tissue. In the intestine, L. lactis-treated asthmatic rats increased Peyer’s patch and goblet cell quantity and mRNA expression of IgA, MUC-2, and claudin. Additionally, intestinal morphological alterations were normalized by L. lactis administration. Splenocyte proliferative response to OVA was abolished, and serum levels of transforming growth factor (TGF)-β were increased by L. lactis treatment. Conclusions: These findings suggest that L. lactis is a potential candidate for asthma prevention, and the effect is mediated by the improvement of intestinal barrier function and systemic TGF-β production.

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Probiotics Prevents Sensitization to Oral Antigen and Subsequent Increases in Intestinal Tight Junction Permeability in Juvenile–Young Adult Rats

Cited by 28 publications

References 51 publications

Bioactive Compounds in Food as a Current Therapeutic Approach to Maintain a Healthy Intestinal Epithelium

Bioactive Compounds in Food as a Current Therapeutic Approach to Maintain a Healthy Intestinal Epithelium

Cinnamaldehyde Promotes the Intestinal Barrier Functions and Reshapes Gut Microbiome in Early Weaned Rats

<b><i>Lactococcus lactis</i></b> NZ9000 Prevents Asthmatic Airway Inflammation and Remodelling in Rats through the Improvement of Intestinal Barrier Function and Systemic TGF-β Production

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