Production of a new wheat cultivar with a different 1B.1R translocation with resistance to powdery mildew and stripe rust

Identification of a novel wheat-Thinopyrum ponticumaddition line revealed with cytology, SSR, EST-SSR, EST-STS and PLUG markers

“…ponticum, where there is a lack of molecular biology information, the transfer of wheat molecular markers to the species is a feasible method for genetic analysis (Dobrovolskaya et al 2009). Over the last two decades, SSR markers have been used extensively as a effective tool for the genetic analysis of alien chromosomes in various species, such as Thinopyrum ponticum (Hu et al 2011), Thinopyrum intermedium (Lin et al 2007), Triticum timopheevii (Uhrin et al 2012), Leymus mollis (Zhao et al 2013) and Secale cereale (Tang et al 2008).…”

“…ponticum specific bands were extracted from gel. The cloning and sequencing of PCR product was conducted as described by Tang et al (2008).…”

Molecular cytogenetic identification of a wheat–rye 1R addition line with multiple spikelets and resistance to powdery mildew

“…In addition, there were many new wheat-rye germplasms and powdery mildew resistance genes from rye were identified and reported. These Pm genes were located on 1R, 2R, 4Rand 6R chromosomes of rye, and their reaction patterns were different from the four known Pm genes Pm7, Pm8, Pm17, and Pm20 derived from rye (Li et al 2004;Hysing et al 2007;Tang et al 2008;Ren et al 2009;Fu et al 2010Fu et al , 2011Fu et al , 2014Wang et al 2009Wang et al , 2010Zhuang et al 2011;An et al 2006). In the present study, wheat cultivar Shaanmai 611 displayed highly susceptible to powdery mildew, wheat-rye addition line N9436B that contained 1R chromosome of Austrian rye showed immunity to powdery mildew.…”

Molecular Detection of the 1BL.1RS Translocation in Hellenic Bread Wheat Cultivars

“…In CIMMYT 70% of spring wheat germplasm carry the translocation; however, this proportion was declined to about 30% in more recent advanced lines (Singh et al 2006). In addition, recent reports state a loss of effectiveness of the resistant genes to biotic stress conditions (Schneider and Molnar-Lang 2008;Tang et al 2008). This is attributed to the wide use of the 1BL.1RS translocation, and it is suggested that new sources of resistant genes must be obtained (Tang et al 2008).…”

“…In addition, recent reports state a loss of effectiveness of the resistant genes to biotic stress conditions (Schneider and Molnar-Lang 2008;Tang et al 2008). This is attributed to the wide use of the 1BL.1RS translocation, and it is suggested that new sources of resistant genes must be obtained (Tang et al 2008). The presence of the 1BL.1RS wheat rye translocation can be detected with biochemical and molecular markers (Xynias et al 2007;Varzakas et al 2014).…”