Production of a thermostable metal-tolerant laccase from Trametes versicolor and its application in dye decolorization

Abstract

Production of laccase using a submerged culture of Trametes versicolor sdu-4 was optimized using a central composite design of the Response Surface Methodology. Optimized conditions gave a laccase yield of 4,213 U/L which was approximately three times of that in basal medium. The laccase was purified to homogeneity using a three-step process. The overall yield of the purification was 58%, with a purification fold of 11.4 and a specific activity of 1320.7 U/mg protein. The molecular mass of the laccase was 60 kDa. The optimum pH values of the enzyme were 2.2, 3.7, and 7 for the oxidations of ABTS, DMP, and syringaldazine, respectively. The enzyme had adaptability to a broad pH range and high temperature and wsa stable at pH 3.0 ~ 10.0. The half-life of this laccase at 70°C was 2.2 h. Methyl red, 2-bromophenol, and 4-bromophenol were oxidized by the purified laccase in the absence of mediators. Purified laccase was effective in the decolorization of several dyes and was not inhibited by Cu 2+ , Mn 2+ , Zn 2+ , Na + , K + , Mg 2+ , Ba 2+ , and Ca 2+ at 5 mM. These excellent characteristics made it a highly attractive candidate for industrial use.

Laccase Production by Trameteshirsuta, Characterization, and Its Capability of Decoloring Chlorophyll

“…The laccase activity in this study was decreased by more than 80% when exposed to high concentration of Fe 2+ (10 mM), which was in accordance with previous reports (Wang et al, 2010;Zhang et al, 2010). These results suggest that laccase from L19 is metal-tolerant (except for Fe 2+ ) resembling the features of those reported fungal laccases (Zhu et al, 2011). However, 10 mM of Fe 2+ did not influence chlorophyll decolorization too much, still yielding 77% decolorization.…”

Induction, purification, and characterization of a thermo and pH stable laccase from Abortiporus biennis J2 and its application on the clarification of litchi juice

“…[29][30][31] The molecular mass of laccase from Abortiporus biennis J2 accords with the range of molecular weights for most of the fungal laccases reported (50,000-90,000). Laccases from Trametes versicolor (60,000), 11) Odontotermes formosanus (65,000), 32) Perenniporia tephropora (63,000), 33) L. lividus (64,800) 34) , and Dichomitus squalens (66,000) 35) have a molecular weight very close to that of Abortiporus biennis J2 lac-case. On the other hand, Leptosphaerulina chartarum laccase has a small molecular weight of 38,000.…”

“…Therefore, searches for new sources of laccases with better properties such as high level of enzymatic activity and more stability are still desirable. 10,11) Litchi (Litchi chinensis Sonn.) is one of the important commercial fruits, which is widely planted in South China.…”

Bioremoval of the synthetic dye malachite green by marine Trichoderma sp

“…The laccase produced by Hypocrea lixii exhibited the molecular weight of the 50 and 75 kDa, similar to those reported for laccases produced by Aspergillus sp. (Youshuang et al 2011). …”