Production of Acinetobacter radioresistens lipase using Tween 80 as the carbon source

Li, Chen-You; Cheng, Chu-Yuan; Chen, Teh-Liang

doi:10.1016/s0141-0229(01)00396-9

Cited by 45 publications

(19 citation statements)

References 8 publications

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“…Tween-80 was better than olive oil and oleic acid, which was similar to observations of Acinetobacter radioresistens lipase production [12]. A possible reason is that high oleic acid concentration could repress enzyme production.…”

Section: Resultssupporting

confidence: 86%

“…Lipase is known to have higher activity at an oil-water interface than in homogeneous solution-a phenomenon known as interfacial activation-thus the hydrolysis rate of water-miscible Tween-80 was slower than that of water-immiscible olive oil by S. marcescens lipase. Using Tween-80 (polyoxyethylene sorbitan monooleate) as sole carbon source, the oleic acid did not accumulate to a concentration high enough to repress enzyme production [2,12]. Tween-80 provided oleic acid for cell growth through a mode of controlled release (via enzymatic hydrolysis) and showed no significant repression of enzyme production, so it was used as the sole carbon source in subsequent cultures.…”

Section: Resultsmentioning

confidence: 99%

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Optimization of Serratia marcescens lipase production for enantioselective hydrolysis of 3-phenylglycidic acid ester

Gao

et al. 2004

J IND MICROBIOL BIOTECHNOL

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Lipase production and cell growth of Serratia marcescens ECU1010 were optimized in shake flasks, with lipase production being enhanced 9.5-fold (4,780 U/l) compared with the initial activity (500 U/l). Optimal carbon and nitrogen sources were Tween-80 and peptone, and the optimal ratio of Tween-80 to peptone was 1:3. The optimized cultivation conditions were 25 degrees C and pH 6.5. Lipase activity, particularly specific activity, could be improved by decreasing the cultivation temperature from 35 to 25 degrees C. Enzyme stability was significantly improved by simple immobilization with synthetic adsorption resin no. 8244. After five reaction cycles, enzyme activity decreased only very slightly, while enantioselectivity of the preparation remained constant, and the ees (enantiomeric excess of the remaining substrate) achieved in all cases was higher than 97%. The resin-8244-lipase preparation can be used for efficient enantioselective hydrolysis of trans-3-(4'-methoxyphenyl)glycidic acid methyl ester [(+/-)-MPGM], a key intermediate in the synthesis of Diltiazem.

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Section: Resultssupporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Optimization of Serratia marcescens lipase production for enantioselective hydrolysis of 3-phenylglycidic acid ester

Gao

et al. 2004

J IND MICROBIOL BIOTECHNOL

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“…With a variety of lipidic sources as inducers, the lipase activity did not increase; instead, they had a negative effect on lipase synthesis (data not shown). It has been reported in literature [7,13] that low level of lipase is produced in natural oil (olive oil and oleic acid) compared to synthetic inducer (Tween-80).…”

Section: Inducer Concentrationmentioning

confidence: 99%

Study of the experimental conditions for the lipase production by a newly isolated strain of Pseudomonas aeruginosa for the enantioselective hydrolysis of (±)-methyl trans-3(4-methoxyphenyl) glycidate

Singh

Kaur

Chakraborti

et al. 2006

Bioprocess Biosyst Eng

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A lipase producing bacterium has been isolated from the soil to enantiospecifically hydrolyze the (+/-)-methyl trans-3(4-methoxyphenyl) glycidate (MPGM), an intermediate in the synthesis of cardiovascular drug, diltiazem. This hydrolysis provided the desired (-)-MPGM in 44% yield with 99% enantiomeric excess. The organism was identified and confirmed as Pseudomonas aeruginosa by 16S rRNA sequencing. The various physiochemical parameters have been optimized for the maximum production of lipase in shake flask. Beef extract was found to be the best nitrogen source for lipase production. The optimized cultivation conditions were 30 degrees C with an initial medium pH 8 in shake flask. Both inoculum age and inoculum concentration have positive effect on the lipase production and (+/-)-MPGM (3 mM) was found to be the optimal inducer.

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“…To gain a balance between high lipase production and low fermentation medium cost, 1.0% of Tween-80 was finally chosen. It was proved that Tween-80, a nonionic surfactant with a molecular structure of polyoxyethylene sorbitan monooleate, could be used as a carbon source in the lipase (SmL) fermentation medium (data not shown), as also reported elsewhere (Li et al 2001). The amount of residual Tween-80 in culture broth was assayed.…”

Section: Effect Of Tween-80 On Lipase Production and Culture Costmentioning

confidence: 66%

Strain improvement of Serratia marcescens ECU1010 and medium cost reduction for economic production of lipase

Zhao

Chen

2009

World J Microbiol Biotechnol

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Industrial strain improvement plays a central role in the commercial development of microbial fermentation processes. The strain of Serratia marcescens ECU1010, a wild-type lipase-producer capable of stereospecific synthesis of a Diltiazem precursor, was subjected to physical mutation involving treatment by UV-irradiation for 30 s. A mutant strain, no. UV-01, showed enhanced lipase production, but lost the capability of producing red pigment (prodigiosin). The variant strain UV-01 had a 2.3-fold higher activity than the wild type and was stable in its enzyme production for ten serial transfers. For reduction of the fermentation medium cost, dried powder of corn steep liquor was used as an inexpensive substitute for beef extract in the medium. Dextrin as an organic carbon source and Tween-80 as an important element were further optimized, respectively. The high primary biodegradation of the Tween-80 by S. marcescens ECU1010 and its variant demonstrated their potential ability of degrading alkyl polyethoxylates to remove harmful nonionic surfactants from polluted effluents and streams. The optimal cultivation time for lipase biosynthesis was 24 h. These optimized compositions resulted in an economic production of lipase by S. marcescens ECU1010 var. UV-01, with a dramatically reduced cost (1/8-1/7 of the initial one) which is more suitable for industrial application.

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Production of Acinetobacter radioresistens lipase using Tween 80 as the carbon source

Cited by 45 publications

References 8 publications

Optimization of Serratia marcescens lipase production for enantioselective hydrolysis of 3-phenylglycidic acid ester

Optimization of Serratia marcescens lipase production for enantioselective hydrolysis of 3-phenylglycidic acid ester

Study of the experimental conditions for the lipase production by a newly isolated strain of Pseudomonas aeruginosa for the enantioselective hydrolysis of (±)-methyl trans-3(4-methoxyphenyl) glycidate

Strain improvement of Serratia marcescens ECU1010 and medium cost reduction for economic production of lipase

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