Production of Bacterial Amylase on some Commercial Materials

El-Leithy, M.A.; Ibrahim, Arsyik; El-Marsafy, M. K.; Kassim, S.A.

doi:10.1016/s0044-4057(73)80067-x

Zentralblatt Für Bakteriologie, Parasitenkunde, Infektionskrankheiten Und Hygiene. Zweite Naturwissenschaftliche Abteilung: All

1973

DOI: 10.1016/s0044-4057(73)80067-x

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Production of Bacterial Amylase on some Commercial Materials

M.A. El-Leithy

Arsyik Ibrahim

M. K. El-Marsafy

et al.

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Cited by 5 publications

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“…The composition and concentration of media greatly affect the growth and production of extracellular amylase in bacteria (5,6,10), yeasts (9,12), and Aspergillus sp. (2).…”

mentioning

confidence: 99%

Culture Conditions for Production of Thermostable Amylase by Bacillus stearothermophilus

Srivastava

Baruah

1986

Appl Environ Microbiol

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Bacillus stearothermophilus grew better on complex and semisynthetic medium than on synthetic medium supplemented with amino acids. Amylase production on the complex medium containing beef extract or corn steep liquor was higher than on semisynthetic medium containing peptone (0.4%). The synthetic medium, however, did not provide a good yield of extracellular amylase. Among the carbohydrates which favored the production of amylase are, in order starch > dextrin > glycogen > cellobiose > maltohexaose-maltopeptaose > maltotetraose and maltotriose. The monosaccharides repressed the enzyme production, whereas inositol and D-sorbitol favored amylase production. Organic and inorganic salts increased amylase production in the order of KCI > sodium malate > potassium succinate, while the yield was comparatively lower with other organic salts of Na and K. Amino acids, in particular isoleucine, cysteine, phenylalanine, and aspartic acids, were found to be vital for amylase synthesis. Medium containing CaC12 2H20 enhanced amylase production over that on Ca2+-deficient medium. The detergents Tween-80 and Triton X-100 increased biomass but significantly suppressed amylase synthesis. The amylase powder obtained from the culture filtrate by prechilled acetone treatment was stable over a wide pH range and liquefied thick starch slurries at 80°C. The crude amylase, after (NH4)2SO4 fractionation, had an activity of 210.6 U mg-'. The optimum temperature and pH of the enzyme were found to be 82°C and 6.9, respectively. Ca2+ was required for the thermostability of the enzyme preparation.

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“…The composition and concentration of media greatly affect the growth and production of extracellular amylase in bacteria (5,6,10), yeasts (9,12), and Aspergillus sp. (2).…”

mentioning

confidence: 99%

Culture Conditions for Production of Thermostable Amylase by Bacillus stearothermophilus

Srivastava

Baruah

1986

Appl Environ Microbiol

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Effect of Starch Induced Bacterial Growth and Amylase Production in Bacillus subtilis

Ensari¹,

Otludil²,

Aytekin³

1995

Starch Stärke

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The amylases produced by a Bacillus subfilis were purified from three different media which occurred nonstarch, starched and after eight hours shocked by starch media. Purified enzymes were compared each other Km, purification steps, thermal stability, pH and bacterial growth. In shocked medium, bacterial growth and amylase production are much more than in starched medium.The enzyme was stable at 65OC in three media. Km values were found different in three media where maltose inhibited the amylase activity. This inhibition is a competitive inhibition. ~ ~ ~ ~ ~ ~~ starchktarke 47 (1995) Nr 8 S. 315-321 0 VCH Verlagsgesellschaft mbH, D-69451 Weinhelm, 1995 0038-9056/95/0808-0315$05 00+ 25/0 3 15

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Efficient production of (R,R)-2,3-butanediol from cellulosic hydrolysate using Paenibacillus polymyxa ICGEB2008

Adlakha¹,

Yazdani²

2014

J Ind Microbiol Biotechnol

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We report here the production of pure (R,R)-2,3-butanediol (2,3-BDO) isomer by the non-pathogenic Paenibacillus polymyxa ICGEB2008 using lignocellulosic hydrolysate as substrate. Experimental design based on Plackett-Burman resulted in identification of Mn and K as most crucial salt elements along with the yeast extract for 2,3-BDO production. Further experiments using Box-Behnken design indicated that both KCl and yeast extract together had major impact on 2,3-BDO production. Optimized medium resulted in 2,3-BDO production with 2.3-fold higher maximum volumetric productivity (2.01 g/L/h) and similar yield (0.33 g/g sugar) as compared to rich yeast extract-peptone-dextrose medium in the bioreactor studies. Considering that the balance substrate was channeled towards ethanol, carbon recovery was close to theoretical yield between the two solvents, i.e., 2,3-BDO and ethanol. Biomass hydrolysate and corn-steep liquor was used further to produce 2,3-BDO without impacting its yield. In addition, 2,3-BDO was also produced via simultaneous saccharification and fermentation, signifying robustness of the strain.

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Production of Bacterial Amylase on some Commercial Materials

Cited by 5 publications

References 10 publications

Culture Conditions for Production of Thermostable Amylase by Bacillus stearothermophilus

Culture Conditions for Production of Thermostable Amylase by Bacillus stearothermophilus

Effect of Starch Induced Bacterial Growth and Amylase Production in Bacillus subtilis

Efficient production of (R,R)-2,3-butanediol from cellulosic hydrolysate using Paenibacillus polymyxa ICGEB2008

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