Production of haploid barley plants from barley*maize and barley*Italian ryegras crosses.

Effect of 2,4-Dichlorophenoxyacetic Acid on the Efficiency of Wheat Haploid Production by theHordeum bulbosumMethod

“…In addition, Suenaga (1990) showed that treatment with 10 and 1,000 mg L -1 2,4-D affected the frequency of embryo formation only slightly but that with 100 mg L -1 2,4-D greatly increased the frequency, in wheat x maize crosses. Furusho et al (1991) reported that 2,4-D at 75 mg L -1 was most effective for obtaining the embryos and plantlets, and that of 50 mg L -1 and 100 mg L -1 was less effective. In previous reports, it was not stated whether or not the treatment with 2,4-D had signifi cant effect on the development of haploid wheat embryos into plantlets.…”

Genomic regions responsible for amenability to Agrobacterium-mediated transformation in barley

“…Dominance of the HN allele was observed at TFA4, TFA7 and TFA9 . This result indicated that there could be negative factors for transformation in GP at these loci, or possibly positive factors in HN, in which regeneration levels are moderate2425. TFA4 was mapped near the locus ari-e .GP, which is a marker of the semi-dwarf phenotype due gibberellin insensitivity2627.…”

Androgenesis: A Fascinating Doubled Haploid Production Process

“…Crossability genes that have been mapped on wheat chromosomes i.e., Kr1 on 5BL, Kr2 on 5AL, Kr3 on 5D, and Kr4 on 1A are not sensitive; thereby, do not create any barriers/hindrance. High frequency of green haploid plants has 2.3 Uniparental Chromosome Removal/Elimination or Wide Hybridization been obtained using maize as pollen donor (wide hybridizer) not only in wheat but also in barley (Furusho et al 1991 ) and triticale (Wedzony et al 1998 ). Genotypic dependence (to some extent), growth and developmental conditions (Campbell et al 2001 ) and emasculation method (Knox et al 2000 ) have been illustrated as major aspects affecting the frequency of green plants to a greater extent in wheat.…”