Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Ralstonia eutropha in whey and inverted sugar with propionic acid feeding

Marangoni, Cintia; Fúrigo, Agenor; Aragão, Gláucia Maria Falcão de

doi:10.1016/s0032-9592(01)00313-2

Cited by 69 publications

(29 citation statements)

References 14 publications

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“…Many studies on the regulation of culture medium pH through pH-stat feeding strategy had been conducted [22,23]. In addition to that, oleic acid and acetic acid had also been added to the culture medium to induce the utilization of propionic acid, thus enhancing the 3HV molar fraction [23].…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis and native granule characteristics of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in Delftia acidovorans

Loo

Sudesh

2007

International Journal of Biological Macromolecules

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Section: Discussionmentioning

confidence: 99%

Biosynthesis and native granule characteristics of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in Delftia acidovorans

Loo

Sudesh

2007

International Journal of Biological Macromolecules

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“…The degree of this expense increase was found to be most inXuenced by the 3HV yield per gram of propionic acid consumed, with the rate of increase lowered when the substrate-speciWc yield was hypothetically raised from 0.2 to 0.5 g 3HV/g propionic acid consumed. Using fermentor-based cultures of W. eutropha KHB-8862, Chung et al [16] determined 3HV yields of 0.5 g/g of levulinic acid consumed, which are relatively high compared to those obtained from propionic acid (0.06-0.13 g/g) in other W. eutropha-based studies [22,49]. In addition to favorable substrate-speciWc yields reported for levulinic acid, its production economics also favor its use as a PHA platform chemical.…”

Section: Economic Considerations For Commercial Pha Productionmentioning

confidence: 99%

Polyhydroxyalkanoate copolymers from forest biomass

Keenan

Nakas

Tanenbaum

2006

J IND MICROBIOL BIOTECHNOL

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The potential for the use of woody biomass in poly-beta-hydroxyalkanoate (PHA) biosynthesis is reviewed. Based on previously cited work indicating incorporation of xylose or levulinic acid (LA) into PHAs by several bacterial strains, we have initiated a study for exploring bioconversion of forest resources to technically relevant copolymers. Initially, PHA was synthesized in shake-flask cultures of Burkholderia cepacia grown on 2.2% (w/v) xylose, periodically amended with varying concentrations of levulinic acid [0.07-0.67% (w/v)]. Yields of poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate) [P(3HB-co-3HV)] from 1.3 to 4.2 g/l were obtained and could be modulated to contain from 1.0 to 61 mol% 3-hydroxyvalerate (3HV), as determined by 1H and 13C NMR analyses. No evidence for either the 3HB or 4HV monomers was found. Characterization of these P(3HB-co-3HV) samples, which ranged in molecular mass (viscometric, Mv) from 511-919 kDa, by differential scanning calorimetry and thermogravimetric analyses (TGA) provided data which were in agreement for previously reported P(3HB-co-3HV) copolymers. For these samples, it was noted that melting temperature (Tm) and glass transition temperature (Tg) decreased as a function of 3HVcontent, with Tm demonstrating a pseudoeutectic profile as a function of mol% 3HV content. In order to extend these findings to the use of hemicellulosic process streams as an inexpensive carbon source, a detoxification procedure involving sequential overliming and activated charcoal treatments was developed. Two such detoxified process hydrolysates (NREL CF: aspen and CESF: maple) were each fermented with appropriate LA supplementation. For the NREL CF hydrolysate-based cultures amended with 0.25-0.5% LA, P(3HB-co-3HV) yields, PHA contents (PHA as percent of dry biomass), and mol% 3HV compositions of 2.0 g/l, 40% (w/w), and 16-52 mol% were obtained, respectively. Similarly, the CESF hydrolysate-based shake-flask cultures yielded 1.6 g/l PHA, 39% (w/w) PHA contents, and 4-67 mol% 3HV compositions. These data are comparable to copolymer yields and cellular contents reported for hexose plus levulinic acid-based shake-flask cultures, as reported using Alcaligenes eutrophus and Pseudomonas putida. However, our findings presage a conceivable alternative, forestry-based biorefinery approach for the production of value-added biodegradable PHA polymers. Specifically, this review describes the current and potential utilization of lignocellulosic process streams as platform precursors to PHA polymers including hemicellulosic hydrolysates, residual cellulose-derived levulinic acid, tall oil fatty acids (Kraft pulping residual), and lignin-derived aromatics.

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“…Pode-se perceber que com exceção dos ensaios 06, 09, 10 e 11 os demais ensaios apresentaram velocidades de crescimento coerentes com os valores encontrados por Oliveira (1999); Marangoni et al (2002); Kim et al (1994) e Schneider (2006, se não estão dentro, encontram-se próximas da faixa 0,19 -0,23 h -1…”

Section: Ensaio Composição H S Os % Duração Fase Lag H Duração Faseunclassified

“…Fonseca (2003), em sua pesquisa utilizando de E. coli recombinante proveniente de inserção de plasmídeos de C. necator, em meio suplementado com amido hidrolisado, soro de queijo e óleo de soja em seus níveis máximos (5 %, 5 % e 5 %), obteve 4,63 g.L -1 como valor de biomassa, no presente estudo, percebeu-se que nas mesmas proporções dos mesmos três suplementos (5 %, 5 % e 5 %) utilizados nos cultivos (ensaios 09, 10 e 11), as maiores quantidades de biomassa produzidas (7,0 g.L , respectivamente) também foram conseguidas e de acordo com Marangoni et al (2000) e Kahar et al (2004), a utilização de suplementos pode interferir na via metabólica proporcionando maior produção de células e resultando em maior produtividade.…”

Section: Ensaio Composição H S Os % Duração Fase Lag H Duração Faseunclassified

Uso De Fontes Renováveis De Carbono Provenientes De Resíduos Das Indústrias De Alimentos E Óleo De Soja Visando À Produção De Poli (3-Hidroxibutirato)

et al. 2013

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Resumo: Este trabalho teve como objetivo utilizar fontes renováveis de carbono para crescimento de Cupriavidus necator visando à produção de poli (3-hidroxibutirato) com menor custo, utilizando como suplementos resíduos provenientes das indústrias processadoras de alimentos tais como bagaço de mandioca e soro de queijo e uma matéria-prima também renovável, o óleo de soja. Os cultivos foram realizados seguindo um planejamento experimental 2³, visando obter nesta etapa a melhor condição de crescimento com os suplementos testados. Os resultados obtidos através do planejamento experimental mostraram que não houve resultados significativamente diferentes, indicando que a utilização de qualquer um destes três suplementos (hidrolisado de bagaço de mandioca, soro de queijo e óleo de soja) não influencia a resposta. Palavras-chave: Poli (3-hidroxibutirato), Cupriavidus necator, suplementação.Abstract: This study aimed to use alternative carbon sources for Cupriavidus necator growth aimed at producing poly (3-hydroxybutyrate) at a lower cost, using supplements like waste from food processing industries such as cassava bagasse and cheese whey and renewable raw material, the soybean oil. The cultures were carried out using an experimental design 2 3 , to obtain the best condition at this stage of growth with the supplements tested. The results obtained from the experimental design showed no significant results, indicating that the use of any of these three supplements (cassava bagasse hydrolysates, milk whey, and soy oil) did not influence the response.

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Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Ralstonia eutropha in whey and inverted sugar with propionic acid feeding

Cited by 69 publications

References 14 publications

Biosynthesis and native granule characteristics of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in Delftia acidovorans

Biosynthesis and native granule characteristics of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in Delftia acidovorans

Polyhydroxyalkanoate copolymers from forest biomass

Uso De Fontes Renováveis De Carbono Provenientes De Resíduos Das Indústrias De Alimentos E Óleo De Soja Visando À Produção De Poli (3-Hidroxibutirato)

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