Progesterone and β-Estradiol Stimulate Seed Germination in Chickpea by Causing Important Changes in Biochemical Parameters

Abstract

Effects of progesterone and β-estradiol on morphologic (germination velocity, root and shoot length) and biochemical (activities of α-amylase, superoxide dismutase, peroxidase and catalase, H2O2 content, lipid peroxidation) parameters during germination and post-germination stages of chickpea seeds were studied. The seeds germinated at various hormone concentrations (10 - 4, 10 - 6, 10 - 9, 10 - 12, 10 - 15 M) were harvested at the end of the 1st, 3rd, and 5th day. With comparison to the control, these hormones caused an increment in the number of germinating seeds at the end of days 1 and 3 by accelerating the seed germination. Root and shoot lengths were augmented by both hormones at all hormone concentrations tested. The highest elongation was recorded in 10 - 6 M progesterone and 10 - 9 - 10 - 12 M β-estradiol. Similarly, activities of α-amylase and superoxide dismutase were increased by all concentrations of both hormones, and maximum increases were obtained with 10 - 6 M progesterone and 10- 9-10- 12 M β-estradiol. In the case of superoxide dismutase activity, not only the H2O2 content but also the peroxidase and catalase activities increased. Lipid peroxidation decreased depending on an increase in the antioxidant enzyme activities. In the present study, it was demonstrated that progesterone and β-estradiol even at low concentrations increase the germination velocity and resistance to stress conditions by changing the activities of some biochemical pathways.

Alleviative Role of Β-Estradiol Against 2,4-Dichlorophenoxyacetic Acid Genotoxicity on Common Bean Genome

“…The studies demonstrated that exogenous application of mammalian sex hormones (such as β-estradiol, progesterone and androsterone) substantially improved plant growth and development, augmented protein and nucleic acid contents, stimulated oxidative enzyme activities, reduced H2O2 content, and the lipid peroxidation (MDA) level under non-stress conditions (Karl and Lauchli 2010;Erdal and Dumlupinar 2010). Also, MSHs also affected the inorganic constituents of plants (Erdal and Dumlupinar 2011a).…”

Impact of Biostimulant and Synthetic Hormone Gibberellic acid on Molecular Structure of Solanum melongena L.

“…The increase in CAT and POX activity in Solanum melongena L might lead to accumulation of toxic amount of H 2 O 2 . Erdal and Dumlupinar (2010) demonstrated that progesterone stimulated antioxidant enzyme activities in germinating seeds of chickpea, maize and bean. Yeast used in the current study was tested to produce IAA with or without addition of tryptophan as precursor of IAA, their productivity increased with addition of tryptophan (Fig.…”

Effects of Progesterone, β-Estradiol, and Androsterone on the Changes of Inorganic Element Content in Barley Leaves

“…These hormones were first detected in plants in the beginning of the nineteenth century [2]. Since then, although many studies have been made about presence, quantities and roles of these hormones in plants, their effect mechanisms have not been exactly elucidated, yet [1][2][3][4][5].…”

“…Many researchers reported that it affected positively plant growth and development when MSH exogenously treated [1][2][3][4][5][6]. There are a lot of reports regarding effects of MSH on both morphological (seed germination, root and shoot lengths, dry and fresh weights) and biochemical (protein and sugar contents, amylase activity, oxidative enzyme activities, H 2 O 2 content, and lipid peroxidation level) parameters in plants.…”

“…Dogra and Thukral [7][8][9] reported that MSH treatment changed N, P, Fe, Na, and K contents of maize and wheat plants. Recently, changes in inorganic constituent of germinating chickpea seeds and chickpea seedlings after MSH treatment have been reported by using wavelength dispersive X-ray fluorescence (WDXRF) spectroscopy technique [2][3][4][5].…”