2021
DOI: 10.1039/d1an00788b
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Progressive use of multispectral imaging flow cytometry in various research areas

Abstract: Multi-spectral imaging flow cytometry (MIFC) has become one of the most powerful technologies for investigating general analytics, molecular and cell biology, biotechnology, medicine, and related fields. It combines the capabilities...

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Cited by 5 publications
(4 citation statements)
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“…Aimed to measure intrinsic structural parameters of both D c and D n , imaging flow cytometry was developed to capture fluorescent images of travelling single cells with both membrane and nuclear portions stained [3,4,7,24,25]. Although this approach could report critical structural parameters of single cells based on further image processing, it cannot at the same time collect intrinsic electrical parameters of single cells and thus cannot provide comprehensive evaluations of individual cells.…”
Section: Introductionmentioning
confidence: 99%
“…Aimed to measure intrinsic structural parameters of both D c and D n , imaging flow cytometry was developed to capture fluorescent images of travelling single cells with both membrane and nuclear portions stained [3,4,7,24,25]. Although this approach could report critical structural parameters of single cells based on further image processing, it cannot at the same time collect intrinsic electrical parameters of single cells and thus cannot provide comprehensive evaluations of individual cells.…”
Section: Introductionmentioning
confidence: 99%
“…Hyper- and multi-spectral imaging have become indispensable technologies in fields 1 such as astronomy 2 , earth-observation remote sensing 3 , 4 , food and water quality control 5 , 6 , art conservation and archeology 7 , forensic medicine 8 , surgery 9 , and biomedical assay and diagnosis 10 13 . Methods for measuring the spectrum of the light emitted from each emission point in the field of view are categorized as (1) point scanning (“whisk-broom”) 14 , 15 , (2) line scanning (“push-broom”) 16 18 , (3) wavelength scanning 19 21 , and (4) snapshot 22 25 .…”
Section: Introductionmentioning
confidence: 99%
“…Spectral imaging based on fluorescence detection is often used for multiplex analysis in biomedical assay and diagnosis 10 , 13 . In multiplex analysis, since multiple kinds of analytes (e.g., specific DNAs or proteins) are respectively labeled with different fluorescent dyes, the amount of each analyte present at each emission point in the field of view at each time is quantified by multicomponent analysis, i.e., unmixing the detected spectrum of fluorescences emitted from each emission point at each time 31 , 32 .…”
Section: Introductionmentioning
confidence: 99%
“…Furthermore, automated image recognition with only one channel is much more difficult, whereas multispectral information allows more comprehensive particle characterization. So far a commonly used MIFC instrument is the ImageStream X MkII [14], but similar instruments like the FlowSight or the STEAM flow cytometer exist [14,15].…”
Section: Introductionmentioning
confidence: 99%