Proinflammatory and Antiapoptotic Markers Ofthestages of Acute Enteral Insufficiency

Abstract

The diagnostic significance of the CD3 and Bcl-2 markers for determining the stage of acute intestinal failure was investigated. The authors used their own model. Loperamide at a dose of 0.09 mg/kg/day was used as a drug inducing intestinal failure. The study was carried out on 36 male Wistar rats weighing 250±30 g, comparable in age. The study groups included 12 animals. The length of the villi, the width of the villi, the depth of the crypts, and the width of the crypts were examined morphometrically. An UltraVision Quanto Detection System HRP Polymer (ThermoFisher, USA) was used as a detection system. In group I, there were pronounced inflammation in the form of lymphoplasmacytic infiltration, shortening and thinning of intestinal villi. The number of CD3+ cells, which were located mainly in the center of the upper part of the villi, increased significantly. Bcl-2 expression decreased. In group II, shortening and thinning of intestinal villi progressed. Paneth's cells disappeared. There was a significant decrease in CD3 + and almost complete absence of Bcl-2+ cells (p≤0.05). Thus, in this work, the dynamics of the expression of pro-inflammatory and anti-apoptotic markers and their relationship with a complex of morphological changes in the wall of the small intestine of rats during the modeling of acute enteric failure have been established.

Polyvalent Bacteriophages and Oxygenated Water – Possibilities for Prevention of Surgical Site Infection During Emergency Abdominal Surgery

“…Figure 1. Change in the transluminal enteral pathobiome in study groups (×10 [1][2][3][4][5][6][7][8][9][10] CFU in 1 мl)…”

The clinical and experimental substantiation of bacteriophages and oxygenated preparation application in disseminated peritonitis complicated with enteral insufficiency

“…Согласно одной из основных концепций, при РП, который осложнен синдромом острой энтеральной недостаточности (ОЭН), кишечник рассматривается как «недренированный абсцесс полиорганной недостаточности». Длительное повышение внутрипросветного давления ухудшает жизнеспособность стенки кишечника с последующей диффузией токсичных бактериальных продуктов в брюшную полость, портальную систему, где они абсорбируются, вызывают токсемию и смерть [16]. Воздействие на бактериальное заражение в тонком кишечнике реализуется в основном эмпирической системной антибиотикотерапией, которая зачастую является неэффективной.…”

Results of the Use of Polyvalent Bacteriophages in the Treatment of Infectious Complications in Emergency Abdominal Surgery

“…These cross coupling interactions have a great influence on the formation of the function of the host's immune system and, ultimately, on the outcome of interventions. Evidence suggests that pathogens derived from the gut microbiota can cause postoperative infection while they stealthily travel inside an immune cell and contaminate a remote surgical site (Trojan horse hypothesis) [4][5][6][7][8].…”