Proline-rich cell wall proteins accumulate in growing regions and phloem tissue in response to water deficit in common bean seedlings

Abstract

Plant cell walls undergo dynamic changes in response to different environmental stress conditions. In response to water deficit, two related proline-rich glycoproteins, called p33 and p36, accumulate in the soluble fraction of the cell walls in Phaseolus vulgaris (Covarrubias et al. in Plant Physiol 107:1119-1128, 1995). In this work, we show that p33 and p36 are able to form a 240 kDa oligomer, which is found in the cell wall soluble fraction. We present evidence indicating that the highest accumulation of these proteins in response to water deficit occurs in the growing regions of common bean seedlings, particularly in the phloem tissues. These proteins were detected in P. vulgaris cell suspension cultures, where the p33/p36 ratio was higher under hyperosmotic conditions than in bean seedlings subjected to the same treatment. The results support a role for these proteins during the plant cell response to changes in its water status, and suggest that cell wall modifications are induced in active growing cells of common bean in response to water limitation.

Microarray Analysis of the Abscission-Related Transcriptome in the Tomato Flower Abscission Zone in Response to Auxin Depletion

“…The TPRP-F1 gene was originally identified as a gene encoding a Pro-rich protein preferentially expressed in young tomato fruit (Salts et al, 1991). While the specific functions of TPRP-F1 and related Pro proteins in plants are not yet clear, studies focusing on various members of this plant gene family indicate functions related to different developmental aspects or responses to environmental factors (Goodwin et al, 1996;Holk et al, 2002;Battaglia et al, 2007). In accordance with our observations, a gene encoding a Pro-rich protein was previously identified to be up-regulated specifically in the DZ of Brassica napus pods during dehiscence (Coupe et al, 1994).…”

<italic>GhHyPRP4</italic>, a cotton gene encoding putative hybrid proline-rich protein, is preferentially expressed in leaves and involved in plant response to cold stress

“…Plant proline-rich proteins (PRPs) are divided into three classes: hybrid proline-rich proteins (HyPRPs), PRPs containing several copies of the POVEKPOVXK motif, and NHyPRP proteins [1]. NHyPRP protein has a C-terminal region with a high percentage of proline residues, whereas the extended N-terminus of the protein is essentially devoid of proline residues.…”

Characterization and expression pattern of IbPRP1 and IbPRP2 stress-related genes from sweetpotato

“…Proline-rich proteins are important components of several plant developmental processes (Bozarth et al, 1987;Creelman and Mullet, 1991;Battaglia et al, 2007). PRP genes are represented during seedling growth (Hong et al, 1989), at the early stages of legume root nodule formation (Scheres et al, 1990;van de Wiel et al, 1990;Wilson et al, 1994), in immature maize-embryos (Jose-Estanyol et al, 1992), and in young tomato fruits (Salts et al, 1991).…”

“…Proline-rich proteins are expressed in response to abiotic stress (Bozarth et al, 1987;Creelman and Mullet, 1991;Battaglia et al, 2007). A proline-rich protein of MsPRP2 in Medicago sativa showed stimulation at 400 mM by NaCl as early as 2 h (Deutch and Winicov, 1995).…”