Prophylactic and therapeutic vaccine against Pseudomonas aeruginosa keratitis using bacterial membrane vesicles

Ito, Shin; Nakamura, Jutaro; Fukuta, Michiko; Ura, Takehiro; Teshigawara, Takeshi; Fukushima, Jun; Mizuki, Nobuhisa; Okuda, Kenji; Shimada, Masaru

doi:10.1016/j.vaccine.2021.04.035

Cited by 12 publications

(12 citation statements)

References 51 publications

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“…Another advantage of MVs is their activity [62]. MVs derived from bacterial outer membranes were shown to be effective carriers of immunogenic proteins and were capable of attenuating keratitis caused by Pseudomonas aeruginosa [89]. MVs obtained from outer membranes of Vibrio cholerae, and Neisseria meningitides could prevent infectious challenge and block transmission [60,84,90].…”

Section: Discussionmentioning

confidence: 99%

Immunogenic Properties of MVs Containing Structural Hantaviral Proteins: An Original Study

et al. 2022

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Hemorrhagic fever with renal syndrome (HFRS) is an emerging infectious disease that remains a global public health threat. The highest incidence rate is among zoonotic disease cases in Russia. Most cases of HFRS are reported in the Volga region of Russia, which commonly identifies the Puumala virus (PUUV) as a pathogen. HFRS management is especially challenging due to the lack of specific treatments and vaccines. This study aims to develop new approaches for HFRS prevention. Our goal is to test the efficacy of microvesicles (MVs) as PUUV nucleocapsid (N) and glycoproteins (Gn/Gc) delivery vehicles. Our findings show that MVs could deliver the PUUV N and Gn/Gc proteins in vitro. We have also demonstrated that MVs loaded with PUUV proteins could elicit a specific humoral and cellular immune response in vivo. These data suggest that an MV-based vaccine could control HFRS.

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Section: Discussionmentioning

confidence: 99%

Immunogenic Properties of MVs Containing Structural Hantaviral Proteins: An Original Study

et al. 2022

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“…Similarly, Zhang and colleagues showed that immunization with EVs from Pseudomonas, formulated with aluminum phosphate adjuvant, protected mice challenged with P. aeruginosa in a model of acute lung infection, reducing cytokine secretion, bacterial colonization, and tissue damage [ 81 ]. Likewise, Ito and colleagues successfully used EVs from P. aeruginosa to develop a prophylactic and therapeutic vaccine for corneal infections in C57BL/6 mice [ 82 ]. In their study, the authors injected the EVs intramuscularly into mice before infecting the cornea.…”

Section: Extracellular Vesicles As Biotechnological Toolsmentioning

confidence: 99%

“…It was found that both passive and active immunization significantly affected the infection process by P. aeruginosa. Additionally, it was reported that using the immune sera as a topical treatment for the cornea decreased the bacterial load and clinical score [ 82 ]. Interestingly, although the use of LPS in vaccines can have considerable side effects, in this study, the administration of EVs without modification or removal of LPS did not seem to generate unwanted effects [ 82 ].…”

Section: Extracellular Vesicles As Biotechnological Toolsmentioning

confidence: 99%

“…Additionally, it was reported that using the immune sera as a topical treatment for the cornea decreased the bacterial load and clinical score [ 82 ]. Interestingly, although the use of LPS in vaccines can have considerable side effects, in this study, the administration of EVs without modification or removal of LPS did not seem to generate unwanted effects [ 82 ]. Most studies using well-characterized Pseudomonas antigens for vaccine development in the last 50 years have been unsuccessful [ 83 ].…”

Section: Extracellular Vesicles As Biotechnological Toolsmentioning

confidence: 99%

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Extracellular Vesicles of Pseudomonas: Friends and Foes

Henríquez

Falciani

2023

Antibiotics

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Extracellular vesicles (Evs) are small spherical vesicles capable of transporting molecules (such as proteins, nucleic acids and lipids) from one cell to another. They have been implicated in processes such as cell-to-cell communication, pathogenicity, biofilm formation and metabolism. In parallel, Evs have been proposed as interesting biotechnological tools. In recent years, antibiotic resistance has become a major problem for human health worldwide. A pathogen singled out as among the most lethal antibiotic-resistant organisms is Pseudomonas aeruginosa, an important Gram-negative bacterium that has been extensively studied for the production and characterization of Evs. Here, we describe the advances made in the last decade regarding understanding of the role of Evs in the pathogenicity of Pseudomonas. We also examine the potential of Evs for the development of new treatment strategies.

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“…Vaccination using microbe-derived products to reduce the host inflammatory responses have been tested in animal models and appear promising. [ 136 ]…”

Section: Antimicrobialsmentioning

confidence: 99%

Update on diagnosis and management of refractory corneal infections

Agarwal

Khan

Vanathi

et al. 2022

Indian Journal of Ophthalmology

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Infectious keratitis is a medical emergency resulting in significant visual morbidity. Indiscriminate use of antimicrobials leading to the emergence of resistant or refractory microorganisms has further worsened the prognosis. Coexisting ocular surface diseases, delay in diagnosis due to inadequate microbiological sample, a slow-growing/virulent organism, or systemic immunosuppressive state all contribute to the refractory response of the ulcer. With improved understanding of these varied ocular and systemic factors contributing to the refractory nature of the microbes, role of biofilm formation and recent research on improving the bioavailability of drugs along with the development of alternative therapies have helped provide the required multidimensional approach to effectively diagnose and manage cases of refractory corneal ulcers and prevent corneal perforations or further dissemination of disease. In this review, we explore the current literature and future directions of the diagnosis and treatment of refractory keratitis.

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Prophylactic and therapeutic vaccine against Pseudomonas aeruginosa keratitis using bacterial membrane vesicles

Cited by 12 publications

References 51 publications

Immunogenic Properties of MVs Containing Structural Hantaviral Proteins: An Original Study

Immunogenic Properties of MVs Containing Structural Hantaviral Proteins: An Original Study

Extracellular Vesicles of Pseudomonas: Friends and Foes

Update on diagnosis and management of refractory corneal infections

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