The aim of this research is to show the influence of working conditions in the relationship between the appropriation of ICT and hospital performance in Cameroon. To achieve this goal, a field survey was conducted with a valid random sample of 479 workers from first and second category public hospitals in Cameroon. The data collected were subjected to descriptive and econometric analyzes. The results of inferential analyzes carried out by means of multiple linear regression show that working conditions in hospitals in Cameroon play a mediating role in the relationship between each of the two dimensions of ICT appropriation, which are perceived utility, perceived ease of use and hospital performance. These results, discussed from the perspective of Berbain and Minvielle (2001), Picard (2007), Venkatraman and Camillus (1984) and theorists of the school of human relations such as Emery and Trist (1968), Herzberg (1959) and Mayo (1949), suggest to managers of hospital structures to: ensure the adequacy between work and the physical and psychological capacities of human resources, take care of the quality of their relations with the hierarchy, ensure their comfort and physical security and finally, provide them with stimulating remuneration in order to optimize hospital performance