Protective properties of microcrystalline cellulose in rats with experimental diabetes

Vainshtein, S. G.; Zhulkevich, I. V.; Petropavlovskii, G. A.; Kotelnikova, N. E.

doi:10.1007/bf00840327

Cited by 6 publications

(4 citation statements)

References 3 publications

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“…The summation of immunological changes in the combined pathology exacerbates the course of the main disease, which we have been able to prove. Changes in the immune system are associated with neuroregulatory disorders, activation of chemoattractants and involvement in the process of inflammatory cells, impairment of local and systemic metabolism and hemodynamics [2,11,14,15]. There is no doubt that in peritonitis with DM, immune and metabolic processes run in parallel and underlie common mechanisms associated with the imbalance between the production of pro-inflammatory and antiinflammatory cytokines, as well as with corresponding changes in the activity of neutrophils and macrophages as effector cells [7,8].…”

Section: Resultsmentioning

confidence: 99%

Status of cellular immunity in rats under conditions of acute widespread petitonitis in the setting of diabetes mellitus

2020

Biointerface Res Appl Chem

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Acute generalized peritonitis (AGP) occurs in patients with concomitant pathology, in particular at diabetes mellitus. The severity of peritonitis depends on the adequacy of the immune response. Purpose of research was to study the features of cellular arm of immune response in the organism of experimental animals with simulated AGP in the setting of diabetes mellitus (DM). 56 white rats were used in the experiment, which was divided into three groups: main group – 24 animals with simulated AWP in the setting of DM; comparison group – 24 animals with simulated peritonitis. Animals of the main group AGP were modelled by injecting 10 % of filtered fecal suspension into the abdominal cavity of tested rats at a dose of 0,5 ml per 100 g of body weight. Removal of material for histological examination was performed on 1, 3 and 7 days. Cellular immunity was determined by a method based on the interaction of fluorescently labeed monoclonal antibodies with lymphocyte surface antigens. In both animals, all indicators of cellular immunity gradually decreased from day 1 to day 7 of the experiment, but these changes were more significant in animals with DM. With AGP, the cellular immune response imbalance is more significant in animals with DM than in animals with isolated AGP, which is characterized by a marked statistically significant decrease in the level of CD3+ cells, CD4+ cells, CD16+ cells, and a moderate increase in CD8+ cells. The imbalance of cellular immunity deepens depending on the duration of the lesion. The level of CD3+ cells at 1, 3, and 7 day in animals with AGP in the setting of DM was 53,77%, 60,48%, and 62,1 %, respectively, lower than the level in the group with animals with AGP. The prolonged imbalance of cellular immunity indices in experimental animals with simulated AGP in the setting of DM indicates not only the occurrence of secondary immunodeficiency, but also significant depletion of the body’s immune forces compared to animals with AGP.

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Section: Resultsmentioning

confidence: 99%

Status of cellular immunity in rats under conditions of acute widespread petitonitis in the setting of diabetes mellitus

2020

Biointerface Res Appl Chem

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“…The influence of chemical compounds (about 5000), plants, physical factors, infectious agents, ectoparasites' bites and other insects, skin invasion by helminthes and other agents can lead to skin lesions [1,2]. Disease manifestations mostly depend on the irritant type, intensity and duration of its impact, repeated contacts, localization of the pathological process, physiological condition of tissues, general condition of the body and its protective and adaptive capabilities, whereas the curing depends on the depth and location of the lesion [3,4,5].…”

Section: Introductionmentioning

confidence: 99%

Morphological Characteristics of Acellular Dermal Matrix Manufacturing

et al. 2021

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The aim is to develop a method of the acellular dermal matrix manufacturing from pig’s skin dermis while preserving the native structure. Materials and methods: Combination of physical and chemical effects on the dermis underlies in the process of an acellular dermal matrix manufacturing. Dermal collection of 1.0-1.3 mm thickness in pigs under 1 year of age from the back and partially from the lateral parts of the body was carried out. The 0.3-0.4 mm thickness layer of skin was previously removed from the relevant areas with help of a dermatome, which was physically and chemically treated. The maximum acellularization of the dermal matrix was achieved step-by-step and included four stages of skin processing: 1 – freeze-thaw process; 2 – glycerin dehydration; 3 – osmotic stress; 4 – cell residue removal by detergent. Results: Histological analysis of the of the pig’s skin dermis revealed that after freeze-thaw cycles the collagen scaffold of the dermal matrix maintains its structural organization that was obtained as a result of the first stage of decellularization. On the second stage of decullalarization, the decreased number of fibroblastic cells was indicated. By means of this, the connective tissue elements that are represented by collagen fibers’ multidirectional bundles retained their structural organization. Fibroblasts lysis as basophilic stained elements was revealed in small amount of dermis on the third stage of the decellularization. Washing of lyophilized skin with nonionic detergent sodium dodecyl sulfate the complete absence of fibroblasts, epidermocytes in the hair follicles, endothelial cells in the wall of blood vessels was detected indicating the effectiveness of this reagent in removing residual products. Conclusions: Suggested protocol for decullalarization of the pig’s skin dermis is effective in removing nuclear and cellular structures from dermis. Particular protocols can be modified by increasing the temperature difference or changing the number of freeze-thaw cycles.

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“…The pathogenesis of diabetic angiopathy [19] remains the most pressing and complex problem in diabetology. The use of experimental models of diabetes is necessary both for the study of the pathophysiology of this disease and for the study of the anti-diabetic properties of new compounds [21], as well as the effect of transplantation of endocrine islets to diabetic animals, since it opens the possibility of studying clinical parameters before and after transplantation or treatment [1,3,4,8,[10][11][12]22].…”

Section: Introductionmentioning

confidence: 99%

Pathophysiological model of indirect revascularization in rats with microangiopathy of limbs caused by experimental streptozocin diabetes

Vastyanov

Chekhlova

2019

Rep. of Morph.

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Despite the large number of publications, the model of experimental diabetes after the introduction of streptozotocin remains a subject of lively scientific debate. The purpose of this study was to develop a pathophysiological model of indirect revascularization in rats with microangiopathy of limbs caused by experimental streptozotocin diabetes. Experimental studies were carried out in a chronic experiment on 100 sexually mature Wistar rats weighing 180-250 g. The streptozotocin diabetes model used. After culling animals from increased resistance to pancreatotropic toxicity by the criterion of the absence of hyperglycemia, three experimental groups were formed: Group I (control) – rats with streptozotocin-induced angiopathy without treatment (n=10); II group – rats with streptozotocin-induced angiopathy treated with pentoxifylline (100 mg/kg IP for 10 days) for therapeutic purposes (n=25); III group – rats with streptozotocin-induced angiopathy, which together with the treatment were injected with platelet-rich plasma (in the right hind limb, once, with a volume of 0.2 ml, linearly, retrogradely, from two points) and pentoxifylline (100 mg/kg IP for 10 days) (n=25). The duration of the experiment was 110 days. We studied the level of glycemia, the state of microcirculation, and the degree of pathomorphological changes in the various study groups. Statistical processing was performed by non-parametric methods using software Statistica 10.0. The developed pathophysiological model of indirect revascularization with the introduction of pentoxifylline and plasma enriched with platelets in diabetic angiopathy is adequate to the needs of clinical physiology. It has been shown that the isolated administration of pentoxifylline is inferior to combined therapy by revascularizing activity. The results of the work may be an experimental justification for the feasibility of clinical application of the combination of pentoxifylline and platelets rich plasma in the treatment of diabetic angiopathy, as well as its use in prophylactic purposes in patients with diabetes mellitus.

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Protective properties of microcrystalline cellulose in rats with experimental diabetes

Cited by 6 publications

References 3 publications

Status of cellular immunity in rats under conditions of acute widespread petitonitis in the setting of diabetes mellitus

Status of cellular immunity in rats under conditions of acute widespread petitonitis in the setting of diabetes mellitus

Morphological Characteristics of Acellular Dermal Matrix Manufacturing

Pathophysiological model of indirect revascularization in rats with microangiopathy of limbs caused by experimental streptozocin diabetes

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