Protective role of lycopene on cisplatin-induced changes in sperm characteristics, testicular damage and oxidative stress in rats

Abstract

The aim of this study was to investigate the possible protective role of lycopene on cisplatin (CP)-induced spermiotoxicity using quantitative, biochemical and histopathological approaches. Adult male Sprague-Dawley rats were randomly divided into four groups. The control group received physiological saline; animals in cisplatin group received only cisplatin; pre-treatment group received a 10-day of lycopene before administration of cisplatin while animals in post-treatment group received a 5-day of lycopene following administration of cisplatin. Cisplatin (7 mg kg(-1)) was intraperitoneally (i.p.) injected as a single dose and lycopene (4 mg kg(-1)) was administered by gavage in corn oil. Traits of reproductive organs; sperm characteristics, testicular histological findings, plasma testosterone levels and the testicular tissue oxidative status were determined. Administration of cisplatin to rats decreased sperm concentration (p < 0.05) and sperm motility (p < 0.001), increased total abnormal sperm rates (p < 0.05) as compared with the control group. While a marked normalization was achieved only in sperm concentration with lycopene in pre-treatment group, significant normalizations were achieved in the sperm concentration, sperm motility, total abnormal sperm rates in post-treatment group. No significant differences in levels of testosterone were observed among all groups. An increase in testes malondialdehyde concentrations (p < 0.05) and glutathione peroxidase activities (p < 0.001) were detected while significant decreases in glutathione levels (p < 0.001) in cisplatin alone group when compared to control group. While pre-treatment with lycopene restoring only malondialdehyde concentrations, its post-treatment caused normalization in both malondialdehyde and glutathione levels when compared with the cisplatin alone group. On the other hand, significant increases were determined in GSH-Px activities in all experimental groups when compared with the control group. Although the mechanism is not clear, the results from this experimental study suggest that the lycopene have a possible protective effect against cisplatin-induced spermiotoxicity, effect of giving lycopene after cisplatin being superior to the giving it before cisplatin.

Cisplatin-Induced Ovarian Cytotoxicity and the Modulating Role of Aqueous Zest Extract of Citrus limonium (AZECL) in Rat Models

“…Mathews [54] reported that the damage occurred in the cell membrane by hydroxyl radicals induced oxidation. This could also explain the marked degeneration, atrophied ovary and decrease number of viable follicles reported in this study which are in line with several reports [23,25,[55][56][57].…”

Lycopene Protects the Diabetic Rat Kidney Against Oxidative Stress-mediated Oxidative Damage Induced by Furan

“…All chemicals and reagents were used of analytical grade. Lycopene and furan were dissolved in corn oil according to studies of Ateşşahin et al and Hamadeh et al (Hamadeh et al 2004;Ateşşahin et al 2006). Thirty-five adult male Wistar rats (300 -320 g, 120 days old) were used for this study.…”

“…For furan, lycopene and STZ, doses used in this study were chosen according to previous studies (Hamadeh et al 2004;Ateşşahin et al 2006;Schmatz et al 2009). Rats were weighed and randomly allocated into five groups of seven individuals: control group (was fed with 1 mL/kg bw corn oil via gavage for 28 days), diabetic control group (was fed with 1 ml/kg bw/day corn oil via gavage for 28 days), diabetic lycopene group (was given lycopene by gavage at dose of 4 mg/kg bw for 28 days), diabetic furan group (was fed with 40 mg/kg bw by gavage for 28 days), diabetic furan+lycopene group (was fed with 40 mg/kg bw ml/kg bw furan and 4 mg/kg bw lycopene via gavage for 28 days).…”

Effects of Grape seed Extract and Origanum Onites Essential Oil on Cisplatin-Induced Hepatotoxicity in Rats

“…Recent studies have shown that different natural antioxidants such as vitamin C, vitamin E, ellagic acid, nigella sativa, lycopen, caffeic acid phenethyl ester prevent cisplatin-induced toxicity. 13,28,[32][33][34][35] In this study, we investigated the effects of the natural antioxidants GSE and OOEO in cisplatin-treated rats by microscopic examination and biochemical assays.…”