Protective role of vitamin E against valproic acid-induced cytogenotoxicity and hepatotoxicity in mice

Introduction: Cardiovascular diseases are one of the most common diseases in the world. It is essential to find an efficient natural protective agent against the myocardial damage. Aim: To determine the protective role of different doses of vitamin E against isoproterenol-induced myocardial damage. Material and Methods: Fifty adult male albino rats were divided into four main groups: Control group (I), vitamin E-treated group (II) equally divided into 2 subgroups; subgroup (IIa) received Vit E (50mg/kg) and subgroup (IIb) received Vit E (100mg/kg) for one month, isoproterenol-treated group (III) received 3ml normal saline orally for one month and isoproterenol (150mg/kg) intraperitoneally (IP) in the last two days of that month. Vitamin E and isoproterenol-treated group (IV) equally divided into two subgroups; Subgroup (IVa) received vitamin E (50mg/kg) orally for one month and isoproterenol (150mg/kg) IP in the last two days of that month and subgroup (IVb) received vitamin E (100mg/kg) orally for one month and isoproterenol (150mg/kg) IP in the last two days of that month. Heart specimens were processed for light and electron microscopic studies. Results: Rats injected by isoproterenol developed structural changes in the myocardium in the form of fragmentation of the myofibrils, vacuolated destroyed mitochondria, dilated SER, intracellular and extracellular edema and interstitial mononuclear cellular infiltration. Animals pretreated with vitamin E at a dose of 50mg/kg before injection of isoproterenol revealed minimal protection as they showed myocardial damage similar to isoproterenol-treated group. While animals pretreated with vitamin E at a dose of 100 mg/kg before injection of isoproterenol showed minimal microscopic alterations of the myocardium with preservation of the normal structure of the cardiac myocytes. Conclusion: Vitamin E at a high dose (100mg/kg) has a protective role on myocardium against isoproterenol-induced myocardial damage.

show abstract

“…The mitochondria were destroyed and vacuolated with loss of their cristae. This finding coincided with previous studies [21] .…”

Section: Discussionsupporting

confidence: 94%

“…It was administered at a dose of 50 or 100 mg/ kg body weight [21] . The calculated dose was suspended in 3 ml normal saline and given as a single daily dose orally for one month by using stainless steel feeding needles.…”

Section: Drugsmentioning

confidence: 99%

Role of Different Doses of Vitamin E in Protection Against Isoproterenol-Induced Myocardial Damage in Adult Male Albino Rat: A Light and Electron Microscopic Study

Aboraya

Ibrahim

Qureshi

2017

Egyptian Journal of Histology

View full text Add to dashboard Cite

show abstract

“…Several animal studies have suggested the protective effects of vitamin E against hepatotoxic drugs such as valproic acid, phenytoin, and cyclophosphamide . The mechanism of vitamin E against drug‐induced hepatotoxicities is based on exerting the highly prominent oxidative properties and apoptosis prevention.…”

Section: Introductionmentioning

confidence: 99%

Has vitamin E any shreds of evidence in cisplatin‐induced toxicity

Hakiminia

Goudarzi²,

Moghaddas

2019

J Biochem & Molecular Tox

View full text Add to dashboard Cite

Cisplatin is one of the highly consumed and effective antitumor agents whose clinical application is accompanied by nephrotoxicity adverse reaction. Also, other complications such as ototoxicity and hepatotoxicity are a matter of concern. Today, it is suggested that cisplatin‐associated toxicities are mainly induced by free radicals production, which will result in oxidative organ injury. The evidence is growing over the protective effects of antioxidants on cisplatin‐induced adverse reactions especially nephrotoxicity. The possible protective effects of vitamin E and its derivative in cisplatin‐induced nephrotoxicity and ototoxicity are reviewed here at the light of pertinent results from basic and clinical research. Administration of vitamin E alone or in combination with other antioxidant agents could cause amelioration in oxidative stress biomarkers such as decreasing the level of malondialdehyde, reducing serum urea and creatinine, and also enhancing the activities of renal antioxidant enzymes including renal catalase, glutathione‐S‐transferase, and superoxide dismutase. Although the data from most of the studies are in favors of protective effects of vitamin E against cisplatin‐induced toxicity, more clinical trials are needed to clarify the clinical importance of vitamin E administration as an antioxidant during cisplatin therapy in cancer condition.

show abstract

“…These finding are in agreement with those found by Shakya et al [24] , who reported that structural changes/damage in the liver did not occur even at higher SV doses of up to 200 mg/kg/day. However, the study done by Abdella et al [25] found that administration of VPA 100 at a dose of mg/kg/day for three weeks leads to inflammatory cell infiltration, hepatocyte degeneration, highly dilated congested central and portal veins, haemorrhage in hepatic parenchyma, fatty changes in bile ductules in hepatocytes which formed newly.…”

Section: Discussionmentioning

confidence: 98%

“…Vit. E was dissolved in 1% Tween 80 solution at a dose of 100 mg/kg [25] and given orally via gastric lavage for eight consecutive days.…”

Section: • Drug Administrationmentioning

confidence: 99%

Evaluation of Dose Related Structural Changes in Sodium-Valproate-Induced Hepatotoxicity and a Possible Protective Role of Vitamin E in Adult Albino Rats

Abdel-Kareem

Tawfeek

2019

Egyptian Journal of Histology

View full text Add to dashboard Cite

Background: Sodium valproate (SV) is a widely administered antiepileptic drug, although hepatotoxicity is a side effect. Vitamin E (vit. E) being a potent antioxidant agent and essential fat-soluble nutrient that can dramatically decreased this hepatotoxic effect. Aim of the Work:To investigate the histopathological and ultrastructural changes caused by different doses of SV, observe their correlations with liver biomarker levels and assess the defensive role of vit. E against SV-hepatotoxicity. Methods: Sixty male adult albino rats were randomly divided into six groups. Group 1 was treated with normal saline. Group 2 was treated orally with vit. E (100 mg/kg/day). Groups 3, 4 and 5 were treated intraperitoneally with SV at doses of 100, 300 and 500 mg/kg/day, respectively, for 8 consecutive days. Group 6 was treated intraperitoneally with SV (500 mg/kg/ day) and orally with vit. E (100 mg/kg/day) for 8 consecutive days. On the ninth day, blood samples were collected to assess the biochemical markers of the liver, and the results were statistically analysed. The rats were deeply anaesthetized and sacrificed. Liver specimens were carefully dissected, and portions were fixed in 10% formalin solution for histopathological examination; others were fixed in 2.5% glutaraldehyde for ultrastructural study. Results:The liver function among the different groups was found to be significantly changed in dose dependent manner. Histopathological examination showed gradual distortion of hepatic lobular architecture and infiltration of lymphocytic cells. Concerning hepatocyte ultrastructure, SV was a destructive compound for most intracellular organelles. This toxicity was most obvious in the groups treated with higher doses; however, concurrent administration of vit. E with SV provided some hepatoprotection. Conclusion:SV is a destructive compound to the liver architecture, especially in high doses. However, SV damage can be attenuated by concurrent administration of vit. E, which considerably decreases most SV-induced hepatotoxicity.

show abstract

Protective role of vitamin E against valproic acid-induced cytogenotoxicity and hepatotoxicity in mice

Cited by 14 publications

References 35 publications

Role of Different Doses of Vitamin E in Protection Against Isoproterenol-Induced Myocardial Damage in Adult Male Albino Rat: A Light and Electron Microscopic Study

Role of Different Doses of Vitamin E in Protection Against Isoproterenol-Induced Myocardial Damage in Adult Male Albino Rat: A Light and Electron Microscopic Study

Has vitamin E any shreds of evidence in cisplatin‐induced toxicity

Evaluation of Dose Related Structural Changes in Sodium-Valproate-Induced Hepatotoxicity and a Possible Protective Role of Vitamin E in Adult Albino Rats

Contact Info

Product

Resources

About