Protein and carbohydrate extraction from S. platensis biomass by ultrasound and mechanical agitation

Lupatini, Anne Luize; Bispo, Larissa de Oliveira; Colla, Luciane Maria; Costa, Jorge Alberto Vieira; Canan, Cristiane; Colla, Eliane

doi:10.1016/j.foodres.2016.11.036

Cited by 87 publications

(47 citation statements)

References 30 publications

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“…It was found that the highest yield of protein occurs after the destruction of cells under high pressure using water extraction, followed by chemical, ultrasonic and mechanical treatments. Lupatini et al [ 56 ] presented a method for extracting proteins and polysaccharides from Spirulina platensis biomass using the ultrasonic treatment and mechanical stirring under alkaline conditions. Under optimized extraction conditions, when treated with ultrasound for 33–40 min and stirring for 40–55 min, the protein yield was 75.76% and polysaccharides yield was 41.52%.…”

Section: Microalgae Proteins Extraction Methodsmentioning

confidence: 99%

Microalgae: A Promising Source of Valuable Bioproducts

et al. 2020

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Microalgae are a group of autotrophic microorganisms that live in marine, freshwater and soil ecosystems and produce organic substances in the process of photosynthesis. Due to their high metabolic flexibility, adaptation to various cultivation conditions as well as the possibility of rapid growth, the number of studies on their use as a source of biologically valuable products is growing rapidly. Currently, integrated technologies for the cultivation of microalgae aiming to isolate various biologically active substances from biomass to increase the profitability of algae production are being sought. To implement this kind of development, the high productivity of industrial cultivation systems must be accompanied by the ability to control the biosynthesis of biologically valuable compounds in conditions of intensive culture growth. The review considers the main factors (temperature, pH, component composition, etc.) that affect the biomass growth process and the biologically active substance synthesis in microalgae. The advantages and disadvantages of existing cultivation methods are outlined. An analysis of various methods for the isolation and overproduction of the main biologically active substances of microalgae (proteins, lipids, polysaccharides, pigments and vitamins) is presented and new technologies and approaches aimed at using microalgae as promising ingredients in value-added products are considered.

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Section: Microalgae Proteins Extraction Methodsmentioning

confidence: 99%

Microalgae: A Promising Source of Valuable Bioproducts

et al. 2020

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“…After sample preparation, carbohydrate analysis is a very complex field. Usually, after microalgal acid hydrolysis, the total carbohydrate content of the hydrolysate can be determined using colorimetric procedures like the phenol-sulfuric acid [37][38][39] or anthrone-based [40][41][42]. These methods are available, giving excellent and robust results with low effort in a very short time.…”

Section: Carbohydrate Extraction and Analysismentioning

confidence: 99%

Concepts and Trends for Extraction and Application of Microalgae Carbohydrates

Souza¹,

Sanchez-Barrios²,

Rizzetti³

et al. 2020

Microalgae - From Physiology to Application

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The proposed chapter aims to provide a more in-depth explanation of the composition of carbohydrates in microalgae biomass, focusing on separation methods, chemistry, molecular characterization, as well as their application in several areas. The purpose of this review chapter is to show that biological products from microalgae have potential in health, food, and industry applications (materials and biofuel production). Steps for extraction and purification will be discussed, as well as the relationship between the type of microalgae and its composition, as a way of optimizing protocol selection and product making, without breaking down the cell to begin with (total carbohydrate extraction present in the cell). An overall overview of the current and prospective trends and methodologies for the use of microalgae carbohydrate will be included as starting points to shed light on some of the possible issues that currently do not allow the development and feasibility of microalgae biorefineries.

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“…According to the importance of gelation properties and the evident characteristics that proteins of S. platensis might present, the aim of this study was to apply a Fractional Factorial Design (FFD) to evaluate the effects of four independent variables (protein concentrate, pH, temperature and heating time) on gelation capacity of S. platensis protein concentrate previously obtained by sonication and mechanical agitation (Lupatini et al, 2017a).…”

Section: Introductionmentioning

confidence: 99%