Protein covalent immobilization via its scarce thiol versus abundant amine groups: Effect on orientation, cell binding domain exposure and conformational lability

Ba, Ousmane M.; Hindié, Mathilde; Marmey, Pascal; Gallet, Olivier; Anselme, Karine; Ponche, Arnaud; Duncan, A.C.

doi:10.1016/j.colsurfb.2015.06.009

Cited by 23 publications

(20 citation statements)

References 42 publications

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“…As described in Fig. 1, the amino groups (lysyl moieties) of FN can react with active ester functions present on the surfaces, while its sulfhydryl groups (cysteyl moieties) enable a reaction with maleimidyl groups on the surfaces [37,[49][50][51]. The successful grafting of the eight crosslinkers and subsequently of FN was confirmed by X-ray Photo-electron Spectroscopy (XPS), contact angle measurements, and Atomic Force Microscopy (AFM).…”

Section: Introductionmentioning

confidence: 87%

Effect of linking arm hydrophilic/hydrophobic nature, length and end-group on the conformation and the RGD accessibility of surface-immobilized fibronectin

Vanslambrouck

Chevallier

Guay‐Bégin

et al. 2020

Materials Science and Engineering: C

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Section: Introductionmentioning

confidence: 87%

Effect of linking arm hydrophilic/hydrophobic nature, length and end-group on the conformation and the RGD accessibility of surface-immobilized fibronectin

Vanslambrouck

Chevallier

Guay‐Bégin

et al. 2020

Materials Science and Engineering: C

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“…A study by Ba et al covalently anchored fibronectin via reactions through thiols or primary amines on amine-functionalized polystyrene in an attempt to restrict the orientation of fibronectin on the surface. These reactions increased the retention of fibronectin to the surface, resulting in higher amounts of immobilized fibronectin and a resistance to deformation, but did not increase the availability of binding sites [24]. Klotzsch et al used single-molecule imaging of fibronectin to track the relative distance between four available cysteines labeled with Cy3B and tracked using photobleaching and total internal reflectance spectroscopy.…”

Section: Biological Functionalizationmentioning

confidence: 99%

Surface functionalization and dynamics of polymeric cell culture substrates

Krutty

Schmitt

Gopalan

et al. 2016

Current Opinion in Biotechnology

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The promise of growing tissues to replace or improve the function of failing ones, a practice often referred to as regenerative medicine, has been driven in recent years by the development of stem cells and cell lines. Stem cells are typically cultured outside the body to increase cell number or differentiate the cells into mature cell types. In order to maximize the regenerative potential of these cells, there is a need to understand cell-material interactions that direct cell behavior and cell-material dynamics. Most synthetic surfaces used for growth and differentiation of cells in the lab are impractical and cost prohibitive in clinical labs. This review focuses on the modification of low cost polymer substrates that are already widely used for cell culture so that they may be used to control and understand cell-material interactions. In addition, we discuss the ability of cells to exert dynamic control over the microenvironment leading to a more complex, less controlled surface.

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“…However, enzymes may encounter structural change upon immobilization due to protein conformation change at the interface, resulting in a reduction of their biocatalytic activity. There are many factors involved in enzyme conformational changes including the features of enzyme molecules and the support material (size and topography), systematic conditions (e.g., pH and temperature) as well as interaction forces (e.g., polar or hydrophobic/hydrophilic) [4][5][6]. The functionality and stability of the immobilized enzymes in a bioreactor system remain a major challenge in large scale operation.…”

Section: Introductionmentioning

confidence: 99%

Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides

et al. 2020

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Molecular distribution, structural conformation and catalytic activity at the interface between enzyme and its immobilising support are vital in the enzymatic reactions for producing bioproducts. In this study, a nanobiocatalyst assembly, β-galactosidase immobilized on chemically modified electrospun polystyrene nanofibers (PSNF), was synthesized for converting lactose into galacto-oligosaccharides (GOS). Characterization results using scanning electron microscopy (SEM) and fluorescence analysis of fluorescein isothiocyanat (FITC) labelled β-galactosidase revealed homogenous enzyme immobilization, thin layer structural conformation and biochemical functionalities of the nanobiocatalyst assembly. The β-galactosidase/PSNF assembly displayed enhanced enzyme catalytic performance at a residence time of around 1 min in a disc-stacked column reactor. A GOS yield of 41% and a lactose conversion of 88% was achieved at the initial lactose concentration of 300 g/L at this residence time. This system provided a controllable contact time of products and substrates on the nanofiber surface and could be used for products which are sensitive to the duration of nanobiocatalysis.

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Protein covalent immobilization via its scarce thiol versus abundant amine groups: Effect on orientation, cell binding domain exposure and conformational lability

Cited by 23 publications

References 42 publications

Effect of linking arm hydrophilic/hydrophobic nature, length and end-group on the conformation and the RGD accessibility of surface-immobilized fibronectin

Effect of linking arm hydrophilic/hydrophobic nature, length and end-group on the conformation and the RGD accessibility of surface-immobilized fibronectin

Surface functionalization and dynamics of polymeric cell culture substrates

Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides

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