Protein Microarrays with Novel Microfluidic Methods:  Current Advances

Dixit, Chandra Kumar; Aguirre, Gerson R.

doi:10.3390/microarrays3030180

Cited by 14 publications

(7 citation statements)

References 117 publications

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“…Conversely, numerous attempts are being made to address the bulk-matrix challenge by employing microfluidic tools. Examples of such tools are fluidic-integrated microarrays 22,23 and microplate-based tools, 24 bead-based fluidic systems and fluidic nanoarrays. 25 These tools are of low volume and moderate sensitivity that mainly represents biomarker levels under several clinical conditions including cancer.…”

Section: Analytical Consideration and Tool Designingmentioning

confidence: 99%

Electrochemistry-based approaches to low cost, high sensitivity, automated, multiplexed protein immunoassays for cancer diagnostics

Dixit

Kadimisetty

Otieno

et al. 2016

Analyst

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Early detection and reliable diagnostics are keys to effectively design cancer therapies with better prognoses. Simultaneous detection of panels of biomarker proteins holds great promise as a general tool for reliable cancer diagnostics. A major challenge in designing such a panel is to decide upon a coherent group of biomarkers which have higher specificity for a given type of cancer. The second big challenge is to develop test devices to measure these biomarkers quantitatively with high sensitivity and specificity, such that there are no interferences from the complex serum or tissue matrices. Lastly, integrating all these tests into a technology that doesn’t require exclusive training to operate, and can be used at point-of-care (POC) is another potential bottleneck in futuristic cancer diagnostics. In this article, we review electrochemistry-based tools and technologies developed and/or used in our laboratories to construct low-cost microfluidic protein arrays for highly sensitive detection of the panel of cancer-specific biomarkers with high specificity and at the same time have the potential to be translated into a POC.

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Section: Analytical Consideration and Tool Designingmentioning

confidence: 99%

Electrochemistry-based approaches to low cost, high sensitivity, automated, multiplexed protein immunoassays for cancer diagnostics

Dixit

Kadimisetty

Otieno

et al. 2016

Analyst

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“…The immobilization of proteins in the surface of microfluidic chips is a frequently performed task in the applications of Lab-on-Chip technology (e.g., [31,32,33,34]).…”

Section: Resultsmentioning

confidence: 99%

Workshop, Cost-Effective and Streamlined Fabrications of Re-Usable World-To-Chip Connectors for Handling Sample of Limited Volume and for Assembling Chip Array

Lue

Kuo

2018

Sensors

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The world-to-chip interface is an essential yet intriguing part of making and employing microfluidic devices. A user-friendly connector could be expensive or difficult to make. We fabricated two ports of microfluidic chips with easily available materials including Teflon blocks, double adhesive films, coverslips, and transparency films. By using a mini grinder, coverslips were drilled to form small holes for the fluid passages between port and chip. Except for the double adhesive films, the resultant ports are durable and re-useable. The DK1 port, contains a mini three-way switch which allows users to handle fluid by a tube-connected pump, or by a manual pipette for the sample of trace amount. The other port, the DK2 port, provides secured tube-connections. Importantly, we invented a bridge made of craft cutter-treated transparency films and double adhesive films to mediate liquid flow between DK2 port and chip. With the use of a bridge, users do not need to design new ports for new chips. Also, individual chips could be linked by a bridge to form a chip array. We successfully applied DK1 port on a microfluidic chip where green fluorescent protein was immobilized. We used DK2 port on an array of fish chips where the embryos of zebra fish developed.

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“…Lab-on-a-chip (LOC) has also been used for monitoring disease states, clinical diagnostics (the detection of disease) (Millington et al 2014), and biochemical analysis by quantitative (numerical) and qualitative (i.e., determining the nature) analysis of chemical substances or proteins from the sample of interest (e.g., cancer markers in blood) (Mirasoli et al 2014). LOC can be used for analysis of proteins, DNA, etc., as in protein microarrays (Dixit and Aguirre 2014) and DNA microarrays (Ohlander 2014). A microarray is a collection of miniaturized tests located on a membrane that allows numerous tests to be carrying simultaneously, or with the purpose of achieve higher throughput rate.…”

Section: Biomedical Nanotechnology In ''Lab-on-a-chip'' Techniquesmentioning

confidence: 99%

Improving “lab-on-a-chip” techniques using biomedical nanotechnology: a review

Gorjikhah

Davaran

Salehi

et al. 2016

Artificial Cells, Nanomedicine, and Biotechnology

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Nanotechnology and its applications in biomedical sciences principally in molecular nanodiagnostics are known as nanomolecular diagnostics, which provides new options for clinical nanodiagnostic techniques. Molecular nanodiagnostics are a critical role in the development of personalized medicine, which features point-of care performance of diagnostic procedure. This can to check patients at point-of-care facilities or in remote or resource-poor locations, therefore reducing checking time from days to minutes. In this review, applications of nanotechnology suited to biomedicine are discussed in two main class: biomedical applications for use inside (such as drugs, diagnostic techniques, prostheses, and implants) and outside the body (such as "lab-on-a-chip" techniques). A lab-on-a-chip (LOC) is a tool that incorporates numerous laboratory tasks onto a small device, usually only millimeters or centimeters in size. Finally, are discussed the applications of biomedical nanotechnology in improving "lab-on-a-chip" techniques.

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Protein Microarrays with Novel Microfluidic Methods: Current Advances

Cited by 14 publications

References 117 publications

Electrochemistry-based approaches to low cost, high sensitivity, automated, multiplexed protein immunoassays for cancer diagnostics

Electrochemistry-based approaches to low cost, high sensitivity, automated, multiplexed protein immunoassays for cancer diagnostics

Workshop, Cost-Effective and Streamlined Fabrications of Re-Usable World-To-Chip Connectors for Handling Sample of Limited Volume and for Assembling Chip Array

Improving “lab-on-a-chip” techniques using biomedical nanotechnology: a review

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