Protein Profiling of the Dimorphic Pathogenic Fungus, Sporothrix schenckii

Zhang, Zhenying; Hou, Binbin; Xin, Yi; Liu, Xiaoming

doi:10.1007/s11046-011-9456-0

Cited by 16 publications

(15 citation statements)

References 25 publications

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“…We previously reported differentially expressed genes between the mycelial and the yeast phases of S. schenckii using 2DE. The expressed sequence tag of spotC homologous to the DRK1 histidine kinase from B. dermatitidis clearly increases in the yeast form of S. schenckii (4).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning, characterization and differential expression of DRK1 in Sporothrix schenckii

Hou

Zhang

Zheng

et al. 2012

International Journal of Molecular Medicine

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The dimorphism of Sporothrix schenckii (S. schenckii) reflects a developmental switch in morphology and lifestyle that is necessary for virulence. DRK1, a hybrid histidine kinase, functions as a global regulator of dimorphism and virulence in Blastomyces dermatitidis (B. dermatitidis) and Histoplasma capsulatum (H. capsulatum). The partial cDNA sequence of DRK1 of S. schenckii, designated SsDRK1, was obtained using degenerate primers based on the conserved domain of the DRK1 of other fungi. The complete cDNA sequence of SsDRK1 was obtained by 5' and 3' RACE. The full-length cDNA is 4743 bp in size and has an open reading frame (ORF) of 4071 bp, encoding 1356 amino acid residues. The predicted molecular mass of SsDRK1 is 147.3 kDa with an estimated theoretical isoelectric point of 5.46. The deduced amino acid sequence of SsDRK1 shows 65% identity to that of B. dermatitidis. The SsDRK1 was predicted to be a soluble histidine kinase and to contain three parts: sensor domain, linker domain and functional domain. Quantitative real-time RT-PCR revealed that SsDRK1 was more highly expressed in the yeast stage compared with that in the mycelial stage, which indicated that the SsDRK1 may be involved in the dimorphic switch in S. schenckii.

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Section: Introductionmentioning

confidence: 99%

Molecular cloning, characterization and differential expression of DRK1 in Sporothrix schenckii

Hou

Zhang

Zheng

et al. 2012

International Journal of Molecular Medicine

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“…The question remains of whether SsSir2 has the same function in the phenotypic switching of S. schenckii similar to the Sir2 ortholog in other dimorphic fungi. In our previous study, the SsSir2 protein expression level in yeast cells was found to be 5.47-fold higher than that in the mycelial phase of S. schenckii (6). In this study, the mRNA expression of SsSte20 in yeast cells of both ATCC10268 and a clinical S. schenckii isolate from a patient with fixed sporotrichosis (data not shown) were higher than in the mycelial ones.…”

Section: Discussionmentioning

confidence: 84%

“…In a recent study of ours, differentially expressed proteins altered by phenotypic switching in S. schenckii were identified using two-dimensional electrophoresis. One of these proteins is homologous to the Sir2 protein from Aspergillus fumigatus and its expression increases in the early yeast form of S. schenckii (6). …”

Section: Introductionmentioning

confidence: 99%

Molecular cloning, modeling and differential expression of a gene encoding a silent information regulator-like protein from Sporothrix schenckii

Hou

Liu

Zheng

et al. 2014

International Journal of Molecular Medicine

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Sporothrix schenckii (S. schenckii) is a dimorphic fungus that produces lymphocutaneous lesions. The signature characteristic of S. schenckii is a temperature-induced phase transition. Silent information regulator (Sir) has been proven to be involved in phenotypic switching in Saccharomyces cerevisiae (S. cerevisiae) and Candida albicans (C. albicans) by organizing chromatin structure. In this study, we isolated and characterized a Sir homologue gene, designated as SsSir2, from the yeast form of S. schenckii. The full-length SsSir2 cDNA sequence is 1753 bp in size and contains an open reading frame of 1329 bp encoding 442 amino acids. The predicted molecular mass of SsSir2 is 48.1 kDa with an estimated theoretical isoelectric point of 4.6. The SsSir2 kinase domain shows a 78% identity with that of Hst2, a Sir2 Ib gene from S. cerevisiae. Three exons and two introns were identified within the 1472-bp SsSir2 genomic DNA sequence of S. schenckii. A three-dimensional model of SsSir2 was constructed using a homology modeling method, and its reliability was evaluated. The active site of SsSir2 was identified by docking simulation, which indicated that several important residues, such as Asn127 and Asp129, play an important role in the histone deacetylase activity of Sir2 family proteins. The differential expression of the SsSir2 in two stages was demonstrated by real-time RT-PCR. The expression of SsSir2 was higher in the yeast stage compared with that in the mycelial one, which indicated that SsSir2 may be involved in the phenotypic switching and morphogenesis of the yeast phase in S. schenckii.

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“…This group also demonstrated an upregulation of the Hsp70 chaperone Hsp88 in the development of the yeast phase of Spor. schenckii at 37 u C; however, the precise role the hsp88 gene remains unclear (Zhang et al, 2012).…”

Section: Dimorphismmentioning

confidence: 99%

Sporothrix schenckii complex biology: environment and fungal pathogenicity

et al. 2014

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Sporothrix schenckii is a complex of various species of fungus found in soils, plants, decaying vegetables and other outdoor environments. It is the aetiological agent of sporotrichosis in humans and several animals. Humans and animals can acquire the disease through traumatic inoculation of the fungus into subcutaneous tissue. Despite the importance of sporotrichosis, it being currently regarded as an emergent disease in several countries, the factors driving its increasing medical importance are still largely unknown. There have only been a few studies addressing the influence of the environment on the virulence of these pathogens. However, recent studies have demonstrated that adverse conditions in its natural habitats can trigger the expression of different virulence factors that confer survival advantages both in animal hosts and in the environment. In this review, we provide updates on the important advances in the understanding of the biology of Spor. schenckii and the modification of its virulence linked to demonstrated or putative environmental factors.

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Protein Profiling of the Dimorphic Pathogenic Fungus, Sporothrix schenckii

Cited by 16 publications

References 25 publications

Molecular cloning, characterization and differential expression of DRK1 in Sporothrix schenckii

Molecular cloning, characterization and differential expression of DRK1 in Sporothrix schenckii

Molecular cloning, modeling and differential expression of a gene encoding a silent information regulator-like protein from Sporothrix schenckii

Sporothrix schenckii complex biology: environment and fungal pathogenicity

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