Proteomic analysis of human follicular fluid associated with successful in vitro fertilization

Shen, Xiaofang; Liu, Xin; Zhu, Ping; Zhang, Yuhua; Wang, Jiahui; Wang, Yanwei; Wang, Wenting; Liu, Juan; Li, Ning; Liu, Fujun

doi:10.1186/s12958-017-0277-y

Cited by 49 publications

(30 citation statements)

References 45 publications

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“…In an ovarian follicle, the follicular antrum is filled with a fluid called follicular fluid (FF) that surrounds the ovum. Oocyte maturation and ovulation are controlled by the microenvironment that is provided by the FF, which has a significant role in fertilization and early embryo development [87,88]. A number of chemical constituents including hormones, proteins, sugars, anti-apoptotic factors, reactive oxygen species, cytokines, and growth factors are present in the FF, which directly or indirectly governs many important oocyte-specific processes [36].…”

Section: Follicular Fluid: a Magical Olc Differentiation Inducermentioning

confidence: 99%

In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded

Bharti

Jang

Lee

et al. 2020

Cells

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In the last few decades, stem cell therapy has grown as a boon for many pathological complications including female reproductive disorders. In this review, a brief description of available strategies that are related to stem cell-based in vitro oocyte-like cell (OLC) development are given. We have tried to cover all the aspects and latest updates of the in vitro OLC developmental methodologies, marker profiling, available disease models, and in vivo efficacies, with a special focus on mesenchymal stem cells (MSCs), induced pluripotent stem cells (iPSCs), and embryonic stem cells (ESCs) usage. The differentiation abilities of both the ovarian and non-ovarian stem cell sources under various induction conditions have shown different effects on morphological alterations, proliferation- and size-associated developments, hormonal secretions under gonadotropic stimulations, and their neo-oogenesis or folliculogenesis abilities after in vivo transplantations. The attainment of characters like oocyte-like morphology, size expansion, and meiosis initiation have been found to be major obstacles during in vitro oogenesis. A number of reports have either lacked in vivo studies or have shown their functional incapability to produce viable and healthy offspring. Though researchers have gained many valuable insights regarding in vitro gametogenesis, still there are many things to do to make stem cell-derived OLCs fully functional.

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Section: Follicular Fluid: a Magical Olc Differentiation Inducermentioning

confidence: 99%

In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded

Bharti

Jang

Lee

et al. 2020

Cells

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“…The coagulation system plays also www.nature.com/scientificreports www.nature.com/scientificreports/ a role in the ovulation process. It influences oocyte delivery to the oviduct by modulation of follicular fluid consistency and its impact on inflammatory cells 54,55 . The central component of the coagulation system -fibrinogen -is overexpressed after maturation in vivo.…”

Section: Discussionmentioning

confidence: 99%

The bovine cumulus proteome is influenced by maturation condition and maturational competence of the oocyte

Walter

Monthoux

Fortes

et al. 2020

Sci Rep

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In vitro maturation (IVM) of oocytes has still a negative impact on the developmental competence of oocytes. Therefore, this study analysed the cumulus proteome of individual cumulus-oocyte complexes (COCs) with and without maturational competence, matured under in vivo or in vitro conditions (n = 5 per group). A novel, ultrasensitive mass spectrometry (MS) based protein profiling approach, using label-free quantification, was applied. The detected cumulus proteome included 2226 quantifiable proteins and was highly influenced by the maturation condition (479 differentially expressed proteins) as well as maturational competence of the corresponding oocyte (424 differentially expressed proteins). Enrichment analysis showed an overrepresentation of the complement and coagulation cascades (CCC), ECM-receptor interaction and steroid biosynthesis in cumulus of COCs that matured successfully under in vivo conditions. Verification of the origin of CCC proteins was achieved through detection of C3 secretion into the maturation medium, with significantly increasing concentrations from 12 (48.4 ng/ ml) to 24 hours (68 ng/ml: p < 0.001). In relation, concentrations in follicular fluid, reflecting the in vivo situation, were >100x higher. In summary, this study identified important pathways that are impaired in IVM cumulus, as well as potential markers of the maturational competence of oocytes.

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“…In our previous retrospective cohort study of 411 non-PCOS female patients undergoing their first IVF-ET treatment, overweight status women had statistically significantly lower number of mature oocytes, fertilization rate, blast formation rate and embryo implantation rate than those of the normal-weight women [16]. Further, in order to better understand folliculogenesis in the ovary, we had applied a proteomic method to investigate the molecular compositions of HFF surrounding the ovum which provides a special milieu for the development of follicle and the maturation of oocytes, and then identified the HFF proteins associated with oocyte maturation and successful IVF outcome [14]. Therefore, we hypothesized that the alteration of HFF proteins of overweight status women might contribute to their poor oocyte quality.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we have adopted a proteomic approach of dual RP-HPLC combined with LC-MALDI TOF/TOF mass spectrometry to investigate the HFF protein profile. A total of 219 unique high-confidence HFF proteins (FDR < 0.01) were successfully identified, which were associated with various molecular functions, such as immunity, complement and coagulation cascade, and transportation, and participated in the implicated interaction networks [14]. Considering the overweight status as a risk factor for women infertility [15], we performed a retrospective cohort research in 411 non-PCOS women patients undergoing their first IVF-embryo transplantation (IVF-ET) treatment.…”

Section: Open Accessmentioning

confidence: 99%

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Human follicular fluid proteome reveals association between overweight status and oocyte maturation abnormality

et al. 2020

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Background: Human follicular fluid (HFF), which is composed by essential proteins required for the follicle development, provides an important microenvironment for oocyte maturation. Recently, overweight status has been considered as a detrimental impact factor on oocyte maturation, but whether HFF proteome could provide protein markers for assessing overweight-based oocyte maturation deficiency is still unknown. Methods: To reveal the HFF-based molecular characteristics associated with abnormal oocyte maturation, an iTRAQbased comparative proteomic analysis was performed to investigate different HFF protein expression profiles from normal weight women and overweight status women. Results: Two hundred HFF proteins were quantified in our data, of which 43% have not been overlapped by two previous publications. Compared with the HFF proteins of normal weight women, 22 up-regulated HFF proteins and 21 down-regulated HFF proteins were found in the overweight status women. PANTHER database showed these altered HFF proteins participated in development, metabolism, immunity, and coagulation, and STRING database demonstrated their complicated interaction networks. The confidence of proteomic outcome was verified by Western blot analysis of WAP four-disulfide core domain protein 2 (WFDC2), lactotransferrin (LTF), prostate-specific antigen (KLK3), fibronectin (FN1), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Further, ELISA assay indicated WFDC2 might be a potentially useful candidate HFF marker for the diagnosis of oocyte maturation arrest caused by overweight status. Conclusions: Our work provided a new complementary high-confidence HFF dataset involved in oocyte maturation, and these altered HFF proteins might have clinical relevance and diagnostic and prognostic value for abnormal oocyte maturation in overweight status women.

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Proteomic analysis of human follicular fluid associated with successful in vitro fertilization

Cited by 49 publications

References 45 publications

In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded

In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded

The bovine cumulus proteome is influenced by maturation condition and maturational competence of the oocyte

Human follicular fluid proteome reveals association between overweight status and oocyte maturation abnormality

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