Proteomic and functional analyses of Nelumbo nucifera annexins involved in seed thermotolerance and germination vigor

Chu, Pu; Chen, Huhui; Zhou, Yuliang; Li, Yin; Ding, Yu; Jiang, Liwen; Tsang, Edward W. T.; Wu, Keqiang; Huang, Shangzhi

doi:10.1007/s00425-011-1573-y

Cited by 70 publications

(57 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…that was up-regulated by heat treatment. Ectopic expression of this lotus annexin in Arabidopsis conferred resistance to heat treatment in seed germination (32). In this study, we discovered that Arabidopsis AtANN1 is an early participant in a plant's response to heat treatment.…”

Section: Discussionmentioning

confidence: 79%

Proteomic Study of Microsomal Proteins Reveals a Key Role for Arabidopsis Annexin 1 in Mediating Heat Stress-Induced Increase in Intracellular Calcium Levels

Wang

et al. 2015

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

To understand the early signaling steps in the response of plant cells to increased environmental temperature, 2-D difference gel electrophoresis was used to study the proteins in microsomes of Arabidopsis seedlings that are regulated early during heat stress. Using mass spectrometry, 19 microsomal proteins that showed an altered expression level within 5 min after heat treatment were identified. Among these proteins, annexin 1 (AtANN1) was one of those up-regulated rapidly after heat-shock treatment. Functional studies show loss-of-function mutants for AtANN1 and its close homolog AtANN2 were more sensitive to heat-shock treatment, whereas plants overexpressing AtANN1 showed more resistance to this treatment. Correspondingly, the heat-induced expression of heat-shock proteins and heat-shock factors is inhibited in ann1/ann2 double mutant, and the heat-activated increase in cytoplasmic calcium concentration (

show abstract

Section: Discussionmentioning

confidence: 79%

Proteomic Study of Microsomal Proteins Reveals a Key Role for Arabidopsis Annexin 1 in Mediating Heat Stress-Induced Increase in Intracellular Calcium Levels

Wang

et al. 2015

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

show abstract

“…Because of the sacred lotus seeds remain viable after hundreds of years, resistance and damage repair in seeds must be extraordinarily effective [24]. Therefore, sacred lotus seeds could be an excellent model for studying the adaptive mechanisms of seeds [25]. …”

Section: Introductionmentioning

confidence: 99%

Small RNA and degradome profiling reveals miRNA regulation in the seed germination of ancient eudicot Nelumbo nucifera

Jin

Qian

et al. 2016

BMC Genomics

View full text Add to dashboard Cite

BackgroundMicroRNAs (miRNAs) play important roles in plant growth and development. MiRNAs and their targets have been widely studied in model plants, but limited knowledge is available concerning this small RNA population and their targets in sacred lotus (Nelumbo nucifera Gaertn.).ResultsIn this study, a total of 145 known miRNAs belonging to 47 families and 78 novel miRNAs were identified during seed germination using high-throughput small RNA sequencing. Furthermore, some miRNA families which have not yet been reported in monocot or eudicot species were detected in N. nucifera, indicating that these miRNAs was divergence from monocots and core eudicots during evolution. Using degradome sequencing, 2580 targets were detected for all the miRNAs. GO (Gene Ontology) and KEGG pathway analyses showed that many target genes enriched in “regulation of transcription” and involved in “carbohydrate”, “amino acid and energy metabolism”. Nine miRNAs and three corresponding targets of them were further validated by quantitative RT-PCR.ConclusionsThe results present here suggested that many miRNAs were involved in the regulation of seed germination of sacred lotus, providing a foundation for future studies of sacred lotus seed longevity. Comparative analysis of miRNAs from different plants also provided insight into the evolutionary gains and losses of miRNAs in plants.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3032-4) contains supplementary material, which is available to authorized users.

show abstract

“…It is known that macromolecules with important cellular functions like DNA (Yakes and Van Houten, 1997) or proteins (Berlett and Stadtman, 1997) are oxidized, but lipid (per)oxidation is an autocatalytic process and, hence, potentially more devastating once triggered. Typically, lipid oxidation is estimated indirectly by measuring malondialdehyde (Stewart and Bewley, 1980;Goel and Sheoran, 2003;Chu et al, 2012), 4-hydroxy-2-nonenal (Perluigi et al, 2012;Spickett, 2013), autoluminescence (Birtic et al, 2011), or fluorescent lipid oxide reaction products (Morita et al, 2016), but these assays do not resolve the oxidation at the level of lipid classes or individual lipids.…”

mentioning

confidence: 99%

Structure Annotation and Quantification of Wheat Seed Oxidized Lipids by High-Resolution LC-MS/MS

2017

View full text Add to dashboard Cite

Lipid oxidation is a process ubiquitous in life, but the direct and comprehensive analysis of oxidized lipids has been limited by available analytical methods. We applied high-resolution liquid chromatography-mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) to quantify oxidized lipids (glycerides, fatty acids, phospholipids, lysophospholipids, and galactolipids) and implemented a platform-independent high-throughput-amenable analysis pipeline for the high-confidence annotation and acyl composition analysis of oxidized lipids. Lipid contents of 90 different naturally aged wheat (Triticum aestivum) seed stocks were quantified in an untargeted high-resolution LC-MS experiment, resulting in 18,556 quantitative mass-to-charge ratio features. In a posthoc liquid chromatography-tandem mass spectrometry experiment, high-resolution MS/MS spectra (5 mD accuracy) were recorded for 8,957 out of 12,080 putatively monoisotopic features of the LC-MS data set. A total of 353 nonoxidized and 559 oxidized lipids with up to four additional oxygen atoms were annotated based on the accurate mass recordings (1.5 ppm tolerance) of the LC-MS data set and filtering procedures. MS/MS spectra available for 828 of these annotations were analyzed by translating experimentally known fragmentation rules of lipids into the fragmentation of oxidized lipids. This led to the identification of 259 nonoxidized and 365 oxidized lipids by both accurate mass and MS/MS spectra and to the determination of acyl compositions for 221 nonoxidized and 295 oxidized lipids. Analysis of 15-year aged wheat seeds revealed increased lipid oxidation and hydrolysis in seeds stored in ambient versus cold conditions. Lipid oxidation is a process inevitably connected to life. Although essential for all respiring organisms, oxygen can form reactive species (ROS). The accumulation of these compounds leads to the phenomenon commonly referred to as oxidative stress in microbes and plant and animal tissues. ROS play a role in the attack against microbial pathogens by host cells (Imlay, 2013), time-dependent death of dormant seeds (Lee et al., 2010), human diseases (Coyle and Puttfarcken, 1993;Giugliano et al., 1996), in aging (Sohal and Weindruch, 1996;Sohal et al., 2002), and in signaling processes (Gilroy et al., 2016;Mignolet-Spruyt et al., 2016) Unlike oxidized fatty acids (FAs), oxidized lipids are hardly available commercially. Targeted liquid chromatography-mass spectrometry (LC-MS) analysis of oxidized lipids using authentic standards is not very common (Hui et al., 2010;Ravandi et al., 2014). Often, oxidized lipids are detected using tandem mass spectrometry (MS/MS), where the collision-induced products of precursor ions (PRs) are scanned for fragments or neutral losses (NLs) that correspond to oxidized acyl residues expected in oxidized lipids (Spickett and Pitt, 2015), and in some cases, higher resolution MS/MS spectra are provided to support the identification (Buseman et al., 2006;Vu et al., 2012).High-resolution mass spectrometry (MS) allows for ...

show abstract

Proteomic and functional analyses of Nelumbo nucifera annexins involved in seed thermotolerance and germination vigor

Cited by 70 publications

References 84 publications

Proteomic Study of Microsomal Proteins Reveals a Key Role for Arabidopsis Annexin 1 in Mediating Heat Stress-Induced Increase in Intracellular Calcium Levels

Proteomic Study of Microsomal Proteins Reveals a Key Role for Arabidopsis Annexin 1 in Mediating Heat Stress-Induced Increase in Intracellular Calcium Levels

Small RNA and degradome profiling reveals miRNA regulation in the seed germination of ancient eudicot Nelumbo nucifera

Structure Annotation and Quantification of Wheat Seed Oxidized Lipids by High-Resolution LC-MS/MS

Contact Info

Product

Resources

About