Proteomic and transcriptomic analysis reveals evidence for the basis of salt sensitivity in Thai jasmine rice (Oryza sativa L. cv. KDML 105)

Jankangram, Wichuda

doi:10.5897/ajb11.1559

Cited by 5 publications

(4 citation statements)

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“…Eight candidate genes of rice encoded proteins involved in the salt stress response were transcriptionally analyzed in un-inoculated plants and those inoculated with wild type or mutant bacteria, under salt and non-salt conditions. The genes were: salT (salt stress responsive protein) 11 , BADH1 (betaine aldehyde dehydrogenase) 12 , NHX1 (Na + /H + antiporter) 13 , SOS1 (salt overlay sensitive 1 protein) 13 , Cam1-1 (calmodulin) 14 , MAPK5 (mitogen activated protein kinase 5) 12 , CuZn-SOD1 (superoxide dismutase) 15 , and CATb (catalase) 12 . Expression profiles of all genes were observed at similar basal level in plants grown under non-salt conditions (Fig.…”

Section: Resultsmentioning

confidence: 99%

Molecular interaction of 1-aminocyclopropane-1-carboxylate deaminase (ACCD)-producing endophytic Streptomyces sp. GMKU 336 towards salt-stress resistance of Oryza sativa L. cv. KDML105

Jaemsaeng

Jantasuriyarat

Thamchaipenet

2018

Sci Rep

117

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1-aminocyclopropane-1-carboxylate deaminase (ACCD)-producing endophytic Streptomyces sp. GMKU 336 and its ACCD-deficient mutant were inoculated into Thai jasmine rice Khao Dok Mali 105 cultivar (Oryza sativa L. cv. KDML105) under salt stress (150 mM NaCl) conditions. The results clearly indicated that Streptomyces sp. GMKU 336 significantly increased plant growth, chlorophyll, proline, K+, Ca+, and water contents; but decreased ethylene, reactive oxygen species (ROS), Na+, and Na+/K+ ratio when compared to plants not inoculated and those inoculated with the ACCD-deficient mutant. Expression profiles of stress responsive genes in rice in association with strain GMKU 336 were correlated to plant physiological characteristics. Genes involved in the ethylene pathway, ACO1 and EREBP1, were significantly down-regulated; while acdS encoding ACCD in Streptomyces sp. GMKU 336 was up-regulated in vivo. Furthermore, genes involved in osmotic balance (BADH1), Na+ transporters (NHX1 and SOS1), calmodulin (Cam1-1), and antioxidant enzymes (CuZn-SOD1 and CATb) were up-regulated; whereas, a gene implicated in a signaling cascade, MAPK5, was down-regulated. This work demonstrates the first time that ACCD-producing Streptomyces sp. GMKU 336 enhances growth of rice and increases salt tolerance by reduction of ethylene via the action of ACCD and further assists plants to scavenge ROS, balance ion content and osmotic pressure.

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Section: Resultsmentioning

confidence: 99%

Molecular interaction of 1-aminocyclopropane-1-carboxylate deaminase (ACCD)-producing endophytic Streptomyces sp. GMKU 336 towards salt-stress resistance of Oryza sativa L. cv. KDML105

Jaemsaeng

Jantasuriyarat

Thamchaipenet

2018

Sci Rep

117

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“…'KDML105' and 'IR29' were grouped, however, their control and salinity treatments were scattered. Jankangram et al (2013) compared leaf lamina proteomes of 'KDML105' and 'Pokkali' under high salt. The study found that although 'KDML105' contains the elevated level of salinityrelated proteins that are also found in 'Pokkali', its posttranscriptional mechanisms for protein expression are not as efficient as that of 'Pokkali', revealing the basis of salt sensitivity in 'KDML105' rice cultivar.…”

Section: Discussion Discussion Discussionmentioning

confidence: 99%

Physiological traits and metabolic profiles of contrasting rice cultivars under mild salinity stress during the seedling stage

JUMPA

Phetcharaburanin

Suksawat

et al. 2023

Not Bot Horti Agrobo

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This study aimed to investigate the effects of mild salinity stress on some physiological traits and metabolic profiles of rice during the seedling stage. Three rice cultivars differing in salinity stress response, ‘KDML105’ (salt-sensitive, local Thai jasmine rice), ‘IR29’ (salt-sensitive rice), and ‘Pokkali’ (salt-tolerant rice), were subjected to mild salinity stress by flood-irrigated with 40 mM NaCl. Salinity stress significantly reduces the growth and biomass of the sensitive cultivars. Electrolyte leakage in ‘KDML105’ and ‘IR29’ also increased significantly while the relative water content (RWC) decreased. However, salinity stress did not affect growth, electrolyte leakage, and RWC in the tolerant cultivar. Salinity significantly increased Na+/K+ ratio in shoot and root of all cultivars. Nevertheless, the increase of Na+/K+ ratio in ‘KDML105’ and ‘IR29’ was higher than that of ‘Pokkali’. The metabolic profile analysis of ‘KDML105’ showed that uracil, L-proline, malonate, L-carnitine, L-lactate, and guanine were significantly increased, but the levels of dihydroxyacetone and glycine were significantly decreased under salinity conditions. In ‘IR29’, L-proline, melatonin, gamma-aminobutyrate, and acetate were significantly increased. In ‘Pokkali’, however, sugar alcohols and sugars were slightly increased when exposed to salinity conditions. The metabolic pathway analysis revealed one involved metabolic pathway of ‘KDML105’: aminoacyl-tRNA biosynthesis, and three involved those of ‘IR29’: arginine proline metabolism, sulfur metabolism, and butanoate metabolism. The difference in the metabolic profile of the salt-tolerance salt-sensitive cultivars provides new insights to identify additional novel biomarkers that will help better understand the mechanisms for salt tolerance in rice.

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“…The identified proteins have been classified into 10 functional categories, including cell structure, cell growth/division, energy metabolism, disease/defense, intracellular traffic, metabolism, protein destination and storage, signal transduction, hypothetical functions, and unknown proteins [28]. Most rice leaf proteomes were obtained from green leaves [29,30,31,32,33,34,35] however, proteomes from purple anthocyanin-containing leaves have never been reported. The proteomic profiling of plants that accumulate anthocyanins, such as grapes [36], and more specifically the mesocarp of vine-ripened grapes have helped to elucidate the biochemical and physiological changes that occur during anthocyanin accumulation and have been of paramount importance in advancing the understanding of berry development and the ripening process [37].…”

Section: Introductionmentioning

confidence: 97%

A comparative proteomic study of white and black glutinous rice leaves

Phonsakhan

Kong-ngern

2015

Electronic Journal of Biotechnology

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Background: Black glutinous rice contains remarkable levels of anthocyanins, which possess anti-oxidative properties and thus have health benefits. The accumulation of anthocyanins in grains of thirty black glutinous rice varieties was measured, and the results revealed that the accumulated anthocyanin content ranged from 0.262 to 2.539 mg/g. Black glutinous rice Br no. 19 was selected, and its leaf protein expression profile was compared with that of white glutinous rice RD 6 using 2D-PAGE, and the protein spots were then directly analyzed after proteolysis by LC-MS/MS. Results: The proteins from the leaves of the two rice varieties were separated using 2D-PAGE and silver stained, and the spots were analyzed using Image Master 2D Platinum version 5 software. The results showed that the protein profiles of these two rice cultivars were different, with at least six protein spots that were detected only in Br no. 19. In addition, seven protein spots accumulated at higher levels in Br no. 19 than in RD 6. Conclusion: The protein spot S1 (AP005098.4) is homologous to the Rc protein. Our results suggest that some of the proteins enriched in Br no. 19 may be involved in anthocyanin synthesis in the black glutinous rice.

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Proteomic and transcriptomic analysis reveals evidence for the basis of salt sensitivity in Thai jasmine rice (Oryza sativa L. cv. KDML 105)

Cited by 5 publications

References 0 publications

Molecular interaction of 1-aminocyclopropane-1-carboxylate deaminase (ACCD)-producing endophytic Streptomyces sp. GMKU 336 towards salt-stress resistance of Oryza sativa L. cv. KDML105

Molecular interaction of 1-aminocyclopropane-1-carboxylate deaminase (ACCD)-producing endophytic Streptomyces sp. GMKU 336 towards salt-stress resistance of Oryza sativa L. cv. KDML105

Physiological traits and metabolic profiles of contrasting rice cultivars under mild salinity stress during the seedling stage

A comparative proteomic study of white and black glutinous rice leaves

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