2006
DOI: 10.1021/ac061886d
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Prototype Amperometric Biosensor for Sialic Acid Determination

Abstract: This paper describes the first report on the development, characterization, and applications of a prototype amperometric biosensor for free sialic acid (SA). The sensor was constructed by the coimmobilization of two enzymes, i.e., N-acetylneuraminic acid aldolase and pyruvate oxidase, on a polyester microporous membrane, which was then mounted on top of a platinum disk electrode. The SA biosensor operation was based on the sequential action of the two enzymes to ultimately produce hydrogen peroxide, which was … Show more

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Cited by 41 publications
(28 citation statements)
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“…Autonomously functioning cells and solid tumours express an altered concentration of carbohydrates, as well as a differentiated metabolism of conjugated sialic acids. This phenomenon seems to be implicated in a wide range of cellular interactions, such as non-inhibition of adhesion, increased tissue invasion and various other procedures that characterise cancer cells and metastatic expansion [8].…”
Section: Discussionmentioning
confidence: 99%
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“…Autonomously functioning cells and solid tumours express an altered concentration of carbohydrates, as well as a differentiated metabolism of conjugated sialic acids. This phenomenon seems to be implicated in a wide range of cellular interactions, such as non-inhibition of adhesion, increased tissue invasion and various other procedures that characterise cancer cells and metastatic expansion [8].…”
Section: Discussionmentioning
confidence: 99%
“…Though it is not at present widely used as such, TSA seems a very promising routine screening marker, either on its own or in combination with well known and established markers [8]. On its own, TSA has not proved useful for the detection of early malignant disease because of its low diagnostic sensitivity, although TSA serum concentration can allow staging of a cancer.…”
Section: Introductionmentioning
confidence: 99%
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“…Recently, a method has been developed and discussed for the determination of sialic acid using an amperometric biosensor via the measurement of hydrogen peroxide [55]. This method has been optimized and applied to biological samples (human sera), with a LOD of 0.01 mM, reproducibility with RSD of 2.3%, and lineal response to 3.5 mM.…”
Section: Amperometric Methodsmentioning
confidence: 99%
“…As biocatalytic reaction rates are often chosen to be first order dependent on the bulk analyte concentration, such steady-state currents are usually proportional to the bulk analyte concentration. Amperometric sensors have often been used on a large scale for detection of analytes such as glucose, lactate (Ohnuki et al, 2007) and salicylic acid (Marzouk et al, 2007). Biosensors utilizing enzymes such as acetylcholinesterase and butyrylcholinesterase have been employed for the rapid detection of organophosphates and carbamates.…”
Section: Electrochemical Biosensorsmentioning
confidence: 99%