Pseudarthrobacter psychrotolerans sp. nov., a cold-adapted bacterium isolated from Antarctic soil

Abstract

A novel cold-tolerant bacterium, designated strain YJ56T, was isolated from Antarctic soil collected from the Cape Burk area. Phylogenetic analysis through 16S rRNA gene sequence similarity revealed that strain YJ56T was most closely related to the genus Pseudarthrobacter , including Pseudarthrobacter oxydans DSM 20119T (99.06 % similarity), Pseudarthrobacter polychromogenes DSM 20136T (98.98 %) and Pseudarthrobacter sulfonivorans ALLT (98.76 %). The genome size (5.2 Mbp) of strain YJ56T was the largest among all the published genomes of Pseudarthrobacter type strains (4.2–5.0 Mbp). The genomic G+C content of strain YJ56T (64.7 mol%) was found to be consistent with those of other Pseudarthrobacter strains (62.0–71.0 mol%). The average nucleotide identity and average amino acid identity values between strain YJ56T and P. sulfonivorans ALLT were estimated at 84.1 and 84.2 %, respectively. The digital DNA–DNA hybridization value between the two strains was calculated to be 28.0 %. This rod-shaped and obligate aerobic strain exhibited no swimming or swarming motility. It had catalase activity but no oxidase activity. Cells grew at 4–28 °C (optimum, 13 °C) and pH 5.0–11.0 (optimum, pH 7.0) and with 0–6.0 % (w/v) NaCl (optimum, 0%) in Reasoner's 2A medium. MK-9 (H2) was the sole menaquinone. Two-dimensional TLC results revealed that the primary polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two glycolipids and phosphatidylinositol. Fatty acid methyl ester analysis showed that anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0, C16 : 0 and iso-C16 : 0 were the major cellular fatty acids in strain YJ56T. Based on phenotypic and genotypic characteristics, strain YJ56T represents a novel species of the genus Pseudarthrobacter , and thus the name Pseudarthrobacter psychrotolerans sp. nov is proposed. The type strain is YJ56T (=JCM 33881T=KACC 21510T).

Root-inhabiting bacterial microbiota of the perennial sweet sorghum cultivar at the maturing stage under cold stress

“…In our previous study, amplicon deep sequencing of the 16S rDNA V3-V4 region revealed that an unclassi ed species of the genus Pseudarthrobacter with approximately 14% relative abundance was potentially psychrotolerant and noticeably enriched in the rhizosphere soil at the maturing stage of the perennial sweet sorghum N778 aerial and primary root (Lu et al, 2022). In this study, OTU1262 was shown to be particularly abundant in the root sample of perennial sweet sorghum N778 during the maturing stage and to be closely related to the well-known cold-adapted bacterium Pseudarthrobacter psychrotolerans YJ56, which was isolated from Antarctic soil (Shin et al, 2020). Based on the combined ML phylogenetic tree of OTU1262 (16S rDNA V5-V7 region) and OTU2574 (16S rDNA V3-V4 region) (Fig.…”

Microorganisms Capable of Producing Polysaccharides from D-Xylose

“…The lipid analysis appeared to capture the characteristic cellular fatty acid content of closely related strains of each species. 11) 12) 13) 14) 15) 16) 17) However, it was not entirely clear due to the lack of standards. Related species of SO_005 ( Papiliotrema laurentii ) was known to produce lipids from D-Xyl, and studies on productivity and lipid composition were investigated.…”

Pseudarthrobacter albicanus sp. nov., isolated from Antarctic soil

“…The phylogenetic trees generated with the maximum-likelihood and maximum-parsimony methods can be seen in Figs S1 and S2 (available in the online version of this article). Based on 16S rRNA gene similarity and the phylogenetic trees, P. sulfonivorans ALL T [25,26], P. siccitolerans 4J27 T [27], P. phenanthrenivorans Sphe3 T [5] and P. psychrotolerans YJ56 T [28] were selected as the reference strains for further analyses under the same conditions.…”

“…phenanthrenivorans Sphe3 T [5] and P. psychrotolerans YJ56 T [28] were selected as the reference strains for further analyses under the same conditions.…”