Pseudomonas indica sp. nov., a novel butane-utilizing species.

Pandey, Kalpana; Mayilraj, Shanmugam; Chakrabarti, Tapan

doi:10.1099/00207713-52-5-1559

International Journal of Systematic and Evolutionary Microbiology

2002

DOI: 10.1099/00207713-52-5-1559

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Pseudomonas indica sp. nov., a novel butane-utilizing species.

Kalpana Pandey¹,

Shanmugam Mayilraj²,

Tapan Chakrabarti³

Abstract: The taxonomic position of two butane-utilizing bacteria was studied using a polyphasic approach. Biochemical and physiological characteristics indicated these to be members of the genus Pseudomonas, showing more similarity to Pseudomonas mendocina than to any other species. The major fatty acids found in these two strains also pointed to their similarity to P. mendocina. On the other hand, DNA-DNA hybridization studies with seven related Pseudomonas species belonging to the gamma-Proteobacteria and the deltaTm… Show more

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Cited by 98 publications

(90 citation statements)

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“…Fatty acid methyl esters were extracted and analysed using the Microbial Identification System (MIDI), as described previously (Sasser et al, 1990;Pandey et al, 2002). The cellular fatty acid profiles of strain BUT-3 T and R. appendicifer 120-1 T are listed in Table 2.…”

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Rhodoligotrophos jinshengii sp. nov., isolated from activated sludge

Deng

Chen

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, non-spore-forming, non-motile, ovoid, aerobic bacterial strain, designated BUT-3 T , was isolated from activated sludge from the wastewater treatment facility of a herbicidemanufacturing plant in Kunshan city, Jiangsu province, PR China. Strain BUT-3 T grew between 15 and 40 6C, with optimum growth at 30 6C. The pH range for growth was between 5.0 and 10.0 (optimum pH 7.0). The range of NaCl concentrations for growth of strain BUT-3 T was 0-7.0 % (w/v), with an optimum of 1.5-3.0 % (w/v). A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strain BUT-3 T clustered closely with Rhodoligotrophos appendicifer 120-1 T (98.32 % similarity), with a bootstrap confidence level of 100 %. The major fatty acids (.5 % of total fatty acids) were C 19 : 0 cyclo v8c, C 18 : 1 v7c, C 16 : 0 , anteiso-C 15 : 0 and iso-C 15 : 0 . Strain BUT-3 T contained ubiquinone Q-10 as the predominant respiratory quinone. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, three unidentified aminolipids (AL1-3), two unknown phospholipids (PL1, 5), four unidentified glycolipids (GL1-4) and two unknown lipids (L1, 2). The G+C content of the genomic DNA was 67.7 mol%. The DNA-DNA relatedness between BUT-3 T and R. appendicifer 120-1 T was 44.1±0.6 %. Based on the polyphasic taxonomic data, strain BUT-3 T should be classified as a representative of a novel species of the genus Rhodoligotrophos, for which the name Rhodoligotrophos jinshengii sp. nov. is proposed. The type strain is BUT-3 T (5CCTCC AB2013083 T 5KACC 17220 T ).The genus Rhodoligotrophos, established recently by Fukuda et al. (2012), belongs to the class Alphaproteobacteria, order Rhizobiales, family Rhodobiaceae. The type strain, Rhodoligotrophos appendicifer 120-1 T , was isolated from samples of freshwater from the bottom of a freshwater lake in the Antarctica. It is an oligotroph partly due to the relatively low levels of nutrients in its ecological niche. In this study, we reported a Rhodoligotrophos-like bacterial strain, designated BUT-3 T , which was isolated from activated sludge. Taxonomic analyses demonstrated that the isolate should be placed into a novel species within the genus Rhodoligotrophos.R. appendicifer 120-1 T was used as a reference strain and was purchased from the Japan Collection of Micro-organisms. Unless indicated otherwise, experiments on morphological, physiological, molecular and chemotaxonomic characteristics were performed on cells grown on 0.256LB medium (l 21 :10.0 g tryptone, 5.0 g yeast extract and 5.0 g NaCl) under aerobic conditions at 30 u C.Strain BUT-3 T was isolated from a soil sample collected from Kunshan city, Jiangsu province, PR China, using the standard dilution plating technique at 30 uC on 0.256LB medium. Unlike R. appendicifer 120-1 T , strain BUT-3 T is a copiotroph, and can grow in LB medium, TSB medium (l 21 :15.0 g tryptone, 5.0 g soy peptone and 5.0 g NaCl), R2A medium (l 21 :0.5 g tryptone, 0.5 g yeast extrac...

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Rhodoligotrophos jinshengii sp. nov., isolated from activated sludge

Deng

Chen

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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“…For cellular fatty acid analysis, strain G5 T and the reference strains were grown on marine agar at 30 uC for 36 h. Fatty acid methyl ester analysis was performed using the Sherlock Microbial Identification System (MIDI) as described by Pandey et al (2002). Freeze-dried cells for other chemotaxonomic analyses were prepared from cultures grown in TSB at 30 u C for 4 days.…”

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Yaniella fodinae sp. nov., isolated from a coal mine

Dhanjal

Ruckmani

Cameotra

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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An orange bacterial strain, designated G5 T , was isolated during the study of the bacterial diversity of a coal mine. The cell wall of strain G5 T contained peptidoglycan type A4a (L-Lys-Gly-L-Glu) and the sugars xylose and mannose. The major menaquinones were MK-8 (45.0 %) and MK-9 (34.0 %) and minor amounts of MK-7 and MK-8(H 2 ) were also found. The major fatty acids were anteiso-C 15 : 0 (44.9 %) and iso-C 15 : 0 (44.2 %). The main cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. 16S rRNA gene sequence analysis showed that strain G5 T was closely related to Yaniella halotolerans YIM 70085 T and Yaniella flava YIM 70178 T (both 96.7 % 16S rRNA gene sequence similarity). The genomic DNA G+C content of strain G5 T was 61.6 mol%. These data and other phenotypic characteristics clearly indicated that strain G5 T represents a novel species of the genus Yaniella, for which the name Yaniella fodinae sp. nov. is proposed. The type strain is G5 T (5MTCC 9846 T 5DSM 22966 T ).

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“…Absence of mycolic acid was demonstrated by TLC (Minnikin & Goodfellow, 1976). Fatty acid methyl esters were extracted and analysed as described by Pandey et al (2002). Chemotaxonomic characters are given in the species description.…”

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Kitasatospora sampliensis sp. nov., a novel actinobacterium isolated from soil of a sugar-cane field in India

Mayilraj

Krishnamurthi

Saha

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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Polyphasic characterization of an actinomycete strain VT-36 T isolated from a sugar-cane field soil sample collected in Punjab State, India, revealed that the strain belongs to the genus Kitasatospora. The strain's chemotaxonomic characters and G+C content of DNA (76?5 mol%) were typical of members of the genus. Analysis of the 16S rRNA gene sequence supported the generic affiliation of the strain and showed that its closest phylogenetic relative was Kitasatospora putterlickiae F18-98 T (=DSM 44665 T ) (98?3 % 16S rRNA gene sequence similarity). The similarities with type strains of all other Kitasatospora species were in the range 95?1-97?0 %. The results of DNA-DNA hybridization showed 54 % relatedness of the isolate and K. putterlickiae F18-98 T . Based on the above data and the phenotypic differences from K. putterlickiae and other Kitasatospora species, it is proposed that the isolate should be classified as the type strain of a novel species, Kitasatospora sampliensis sp. nov., with strain VT-36 T (=MTCC 6546 T = DSM 44898 T =JCM 13010 T ) as the type strain. Strain VT-36 T was isolated from a sugar-cane field soil sample collected at Sampli village, Punjab state, India, by using Streptomyces agar (1?0 % malt extract, 0?4 % yeast extract, 0?4 % glucose, 0?2 % calcium carbonate and 1?2 % agar) complemented with cycloheximide (25 ml ml 21 ) and amphotericin B (10 ml ml 21 ) at 28 u C. The pure culture was maintained as a glycerol suspension (10 % v/v) of spores and mycelial fragments at 270 uC. The reference strain K. putterlickiae MTCC 6643 T was obtained from the Microbial Type Culture Collection (MTCC), Chandigarh, India.Strain VT-36 T was grown on Streptomyces agar for morphological and physiological characteristics. Morphological properties were examined according to the methods reported by Williams et al. (1983). Growth and sporulation were observed on standard media-like actinomycetes isolation agar (0?2 % sodium caseinate, 0?01 % asparagine, 0?4 % sodium propionate, 0?05 % dipotassium phosphate, 0?01 % magnesium sulphate, 0?0001 % ferrous sulphate and 1?5 % agar, pH

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Pseudomonas indica sp. nov., a novel butane-utilizing species.

Cited by 98 publications

References 15 publications

Rhodoligotrophos jinshengii sp. nov., isolated from activated sludge

Rhodoligotrophos jinshengii sp. nov., isolated from activated sludge

Yaniella fodinae sp. nov., isolated from a coal mine

Kitasatospora sampliensis sp. nov., a novel actinobacterium isolated from soil of a sugar-cane field in India

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