Purification and properties of recombinant DNA methyltransferase M2.BstSE of the BstSEI nickase-modification system

Peculiarities of the interaction of the restriction endonuclease BspD6I with DNA containing its recognition site

“…Perevyazova, unpublished data, [34]). Chernukhin et al [35] showed, that purified recombinant M2.BstSEI alone (in the absence of M1.BstSEI) is capable to modify adenine in both DNA strands of double-stranded 5′-GASTC-3′ (S = G or C) sequence. Moreover, the kinetic parameters of DNA methylation by M2.BstSEI (homolog of M2.BspD6I) are similar to those of DNA MTases that recognize the palindromic sites [35].…”

“…Chernukhin et al [35] showed, that purified recombinant M2.BstSEI alone (in the absence of M1.BstSEI) is capable to modify adenine in both DNA strands of double-stranded 5′-GASTC-3′ (S = G or C) sequence. Moreover, the kinetic parameters of DNA methylation by M2.BstSEI (homolog of M2.BspD6I) are similar to those of DNA MTases that recognize the palindromic sites [35]. We expect that one of MTases from R-M systems (like the BspD6I or BstSEI R-M system), containing nicking endonuclease, serves not only for the protection of host DNA from digestion by RE but carries out some other functions, for example, it might take part in DNA repair together with NE.…”