Purslane (Portulaca oleracea) Ethanol Extract decreases hs-CRP levels and Total Score of Renal Tubular Degeneration in Rat Model of Gentamicine-Induced Renal Damage

Abstract

Introduction: Injection of intraperitoneal gentamicin at a dose of 60 mg/kg BW intra-peritoneal in rats for 7 days has been shown to trigger renal tubular degeneration and increase in high sensitivity C-reactive protein (hs-CRP) level. The use of purslane (Portulaca oleracea) to reduce hs-CRP levels and total score of renal tubular degeneration has not been reported.Objective: to determine the effect of purslane ethanol extract on hs-CRP levels and renal tubular degeneration score in gentamicin induced-renal damage in rats.Methods: This was a study using posttest only control group design. Twentyfive male Wistar rats aged 8-12 weeks, weighed 150-200 grams were allocated into 5 groups: normal (Nor-G), without treatment; negative (Neg-G), gentamicin (60 mg/kg BW), intraperitoneally; P-200; P-300 ; P-400 (gentamicin plus purslane extract at a dose of 200, 300, and 400 respectively) for 7 days. The hs-CRP was evaluated using the ELISA method. Total score of renal tubular degeneration was evaluated using modified score of Sarjadi.Results: there was a significant difference in mean hsCRP level and total score of renal tubular degeneration between groups (p <0.05). Posthoc analysis showed that hsCRP level and total score group of P-200, P-300 and P-400 were significantly lower than those of Neg-G, p <0.05. Meanwhile, the levels of hsCRP and total score of renal tubular degeneration in P-200, P-300, P-400, and Nor-G groups were not significantly different (p> 0.05).Conclusion: the administration of purslane ethanol extract at doses of 200, 300, and 400 mg/kg BW for 7 days improve hs-CRP level and total score of tubular degeneration similar to normal.

Decreasing the SGPT level of male wistar rats induced by gentamicin with purslane ethanol extract

“…The research conducted by Ahangarpour et al (2018) shows that the common purslane extract has a protective effect on the pancreas by decreasing hypoglycemic activity and insulin resistance. 6 The research conducted in Indonesia by Saptaningtyas et al (2020) also shows that common purslane ethanol extract can improve the level of high-sensitivity CRP (Hs-CRP) with the total score of male Wistar rats' renal tubular degeneration induced by gentamicin is equal to that in normal condition. 7 The increasing SGPT in the male Wistar rats' serum induced by gentamicin indicates an abnormality in their liver.…”

“…6 The research conducted in Indonesia by Saptaningtyas et al (2020) also shows that common purslane ethanol extract can improve the level of high-sensitivity CRP (Hs-CRP) with the total score of male Wistar rats' renal tubular degeneration induced by gentamicin is equal to that in normal condition. 7 The increasing SGPT in the male Wistar rats' serum induced by gentamicin indicates an abnormality in their liver. Oxidative stress is a key mechanism responsible for liver damage resulting from the administration of gentamicin which results in the increasing level of SGPT.…”