QTL analysis of flooding tolerance in soybean at an early vegetative growth stage

Abstract

Soybean cultivars are sensitive to flooding stress and their seed yields are substantially reduced in response to the stress. This study was conducted to investigate the genetic basis of flooding tolerance at an early vegetative growth stage. Sixty recombinant inbred lines derived from a cross between a relatively tolerant cv. ÔMisuzudaizuÕ and a sensitive cv. ÔMoshidou Gong 503Õ were grown in pots in a vinyl plastic greenhouse in 2002 and 2003. At the two-leaf stage, half of the pots were waterlogged by water placed in plastic containers and adjusted to 5 cm above the soil surface. After 3 weeks of treatment, the pots were returned to the greenhouse and grown until maturity. Flooding tolerance was evaluated by dividing the seed weight of the treated plants by that of the control plants. Quantitative trait loci (QTL) analysis using 360 genetic markers revealed three QTLs for flooding tolerance, ft1 to ft3 in 2002. The ft1 (molecular linkage group C2) was reproducible and an additional four QTLs, ft4 to ft7, were found in 2003. The ft1 had a high LOD score in both years (15.41 and 7.57) and accounted for 49.2% and 30.5% of the total variance, respectively. A large QTL for days to flowering was consistently observed across treatments and years at a similar position to ft1. Comparing the relative location with markers, the maturity gene probably corresponds to E1. Late maturity may have conferred a longer growth period for recovery from flooding stress.

Phenotypic variation in root development of 162 soybean accessions under hypoxia condition at the seedling stage

“…This suggests that shoot phenology may differ between the two Miyagishirome, and Nattou kotsubu under waterlogging (Table 4). These results imply a serious problem for soybean production in Japan, because of the long spell of rainy weather during the time when soybean is sown in fields managed in rotation with paddy rice (Githiri et al, 2006). Many Japanese cultivars were susceptible to hypoxia.…”

“…Long spells of rainy weather overlap during the early vegetative stages of soybean in mainland Japan, leading to waterlogging (Githiri et al, 2006). Root growth and morphology under waterlogging and the resulting hypoxia during these early stages have been verified in soil and hydroponic cultivation systems.…”

“…Soybean is often grown in fields converted from paddy rice or undrained lowlands in parts of monsoon Asia (Kokubun, 2013) such as Japan (Githiri et al, 2006), Viet Nam (Loc et al, 2015), and Indonesia (Ghulamahdi et al, 2016), as well as in other parts of the world, such as the Mississippi Delta of the U.S.A (Linkemer et al, 1998;VanToai et al, 2010) and the lowlands of Brazil's Rio Grande (Pedó et al, 2015). In such areas, soil easily becomes waterlogged after rainfall.…”

Waterlogging-induced changes in fermentative metabolism in roots and nodules of soybean genotypes

“…Soil waterlogging is a common abiotic stress worldwide in cultivated areas and influences the composition and productivity of soybean (Glycine max L. Merril) and most crops species (Jackson and Colmer, 2005;Githiri et al, 2006;Sairam et al, 2009;Kokubun, 2013). In waterlogged soils, gas exchanges between root systems and soil porous spaces are limited due to oxygen diffusion resistance that is around 10,000 times higher in water than in the air (Armstrong et al, 1994;Dongen et al, 2003;Zabalza et al, 2009;Bailey-Serres et al, 2012).…”

Translational Genomics in Agriculture: Some Examples in Grain Legumes

“…Resistance to biotic stresses Angular leaf spot QTL 22-64 Oblessuc et al, 2012;Teixeira et al, 2005 (Khedikar et al, 2010;Liang et al, 2009a;Qin et al, 2012;Sujay et al, 2012;Wang et al, 2013 (Gautami et al, 2012b;Ravi et al, 2011;Varshney et al, 2009b) Gautami et al, 2012a;Liang et al, 2009;Ravi et al, 2011;Selvaraj et al, 2009;Shirasawa et al, 2012;Varshney et al, 2009b) (Continued on next page) (Liang et al, 2009;Pandey et al, 2012c;Sarvamangala et al, 2011;Selvaraj et al, 2009;Shirasawa et al, 2012) Pea Plant height QTL 19-65 (Tullu et al, 2008;Tar'an et al, 2003b;Dirlewanger et al, 1994) (Atibalentja et al, 2005;Guo et al, 2006;Guo et al, 2008;Han et al, 2008;Huynh et al, 2010;Kazi et al, 2008;Ruben et al, 2006;Shi et al, 2008;Silva and Danielle, 2008;Vuong et al, 2008;Wang et al, 2010;Winter et al, 2007) (Continued on next page) Funatsuki et al, 2005;Githiri et al, 2006;Githiri et al, 2007;…”