Qualitative and Quantitative Analysis of Volatile Components of Zhengtian Pills Using Gas Chromatography Mass Spectrometry and Ultra-High Performance Liquid Chromatography

Liu, Cui-Ting; Zhang, Min; Yan, Ping; Liu, Hai-chan; Liu, Xing-yun; Zhan, Renya

doi:10.1155/2016/1206391

Cited by 7 publications

(6 citation statements)

References 17 publications

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“…However, the pharmacodynamic mechanisms of Zhengtian Pill are still unclear. The main reason is that Zhengtian Pills is a drug containing 15 kinds of medicinal herbs [17]. It also requires a large amount of research to determine which components play a role in specific diseases.…”

Section: Introductionmentioning

confidence: 99%

The Antioxidative Action of ZTP by Increasing Nrf2/ARE Signal Pathway

Yang

Yuan

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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So far, more than 25,000 brain diseases have been shown to be related to oxidative stress. Excessive free radicals and reactive oxygen species (ROS) can attack cells resulting in dysfunctional proteins, lipids, and nucleic acid, finally leading to imbalance of energy metabolism, cell death, gene mutation, and immune reaction. Therefore oxidative stress plays an important role in neuronal diseases. As a traditional Chinese medicine, Zhengtian Pill (ZTP) was reported to have the ability to reduce the blood viscosity of migraine model rats, with increased beta-endorphin, serotonin, adrenaline, and dopamine in brain tissue. Moreover ZTP can effectively accelerate blood circulation and attenuate blood coagulation. However, the molecular mechanisms of ZPT are still unclear. Through the behavioral test we found that ZTP can significantly improve depression-like behavior induced by LPS when rat was treated with ZTP (L 0.17 g/kg, M 0.34 g/kg, and H 0.7 g/kg) intraperitoneal injection once a day for 30 consecutive days. And ZTP can resist oxidative stress (>72 h) for a longer time. And ZTP can promote the levels of ATP and SOD and reduce the levels of ROS and MDA in the brain. At the same time, ZTP can have antioxidant stress through increasing the expression level of Nrf2/HO-1/P38. These results show that ZTP may be a potential antioxidant stress drug for variety of diseases associated with oxidative stress injury.

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Section: Introductionmentioning

confidence: 99%

The Antioxidative Action of ZTP by Increasing Nrf2/ARE Signal Pathway

Yang

Yuan

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…[13] GC–MS is a combined analytical method that use the features of gas–liquid chromatography and mass spectrometry to identify the active volatile compounds present in the plant solvent extracts. [14] In this study, a total of 29 volatile compounds were predicted from the hexane extract of E. crispa leaves by GC–MS analysis with tetracosane (14.98%); dodecane (10.76%); 2-ethyl-1-decanol (8.00%); tridecane (7.53%), 4,5,6,8-Ptetramethoxy-2,3-dihydroindeno[1,2,3-ij]isoquinolin-9-ol (6.99%); diphenylvinylphosphine (6.38%); squalene (5.85%); triacontane (5.27%); and 2,6-dimethylheptadecane (5.02%) were occupying the highest peak areas compared with rest of the compounds in gas chromatogram analysis. These phytochemicals possess many biological activities such as antioxidant, antimicrobial, antidiabetic, and anticancer.…”

Section: Discussionmentioning

confidence: 99%

Screening of potential phytocompounds from Euclea crispa (Thunb.) leaves targeting human epidermal growth factor receptor 2 (HER2) signaling pathway

Palanisamy

Ashafa

2019

J Pharm Bioall Sci

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A BSTRACT Background: Overexpression of human epidermal growth factor receptor 2 (HER2) plays an important role in the development and progression in a variety of cancers and it is a novel therapeutic target for breast cancer and ovarian cancer. Euclea crispa ( E. crispa ) is a South African medicinal plant in the family Ebenaceae used in the management of different human diseases and disorders. Aims: The aim of this study was to evaluate the potential inhibitors against HER2 from hexane extract of E. crispa leaves. Materials and Methods: Chemical fingerprinting method was used to identify the presence of natural compounds from the extract whereas their inhibitory activities were analyzed by molecular docking analysis against HER2. Absorption, distribution, metabolism, and excretion (ADME) properties also predicted to establish the pharmacokinetics and pharmacodynamics profiles of the selected compounds. Results: The molecular docking analysis expressed that phenyl glucuronide, hydrocortisone acetate, and 6-(4,6-dioxo-1,4,5,6-tetrahydropyrimidin-2-yl-amino)hexanoic acid trifluoroacetate possess good inhibitory activities with good glide score of −6.63, −5.41, and −5.40 and glide energy of −35.03, −42.51, and −31.38 kcal/mol, respectively when compared with standard Food and Drug Administration–approved drug and other compounds. All the screened compounds were within the acceptable and permissible limits of ADME properties. Conclusion: Thus, from this study it can be concluded that, these screened natural compounds from E. crispa leaves may serve as potential inhibitors for HER2 and they might lead to development of new therapeutic agents against cancer and its associated complications.

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“… 13 While qualitative and quantitative analysis were done by using Gas chromatography and Mass spectroscopy (5977A Series GC/MSD System, Agilent Technologies, Inc., USA). 14 …”

Section: Methodsmentioning

confidence: 99%

Evaluation of Phytochemical Contents, Antimicrobial, and antioxidant Potential of Haloxylon Griffithii Collected From Northern Region of Balochistan, Pakistan

Asif

Ali²,

Mazhar

et al. 2023

Dose-Response

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Background The therapeutic potential of Haloxylon griffithii found in northern region of Balochistan, so far has been neglected. Purpose The current study was aimed to assess the phytochemicals and pharmacological potential of fractions isolated from H griffithii. Research Design During phytochemicals analysis of H griffithii using GC/MS showed various bioactive compounds like alkaloids, flavonoids, terpenoids, tannins, saponins, and carboxylic acids. In vitro antioxidant activity of H griffithii was determined by 2, 2’- diphenyl-1-picrylhydrazyl (DPPH) assay. Disc diffusion method was used to evaluate the antimicrobial activity. Results The quantitative analysis of ethyl acetate showed highest total flavonoid contents (1.19 ± .05) while ethanol with lowest value (.52 ± .01). The total phenolic contents in ethyle acetate was 1.50 ± .42, whereas ethanol showed lowest value (.77 ± .02). Ethanol exhibited excellent (88.68 ± 3.0) free radical scavenging potential measured by 1,1-diphenyl-2-picryl-hydrazyl radical scavenging assay. For antimicrobial activity, different bacterial and fungal strains like B subtilis, S aureus, E coli, S typhi, C albicans, and A. niger were selected . The essential oil showed maximum % inhibition diameter (9 mm) against B. Subtillus and (5 mm) against C albicans, respectively . The ethyl acetate presented % inhibition diameter (9 mm) against S aureus and (6 mm) against A niger. Anti-urease activity also showed positive response. Conclusions The presence of high (%) bioactive compounds with great therapeutic potential suggest that H griffithii can be used as natural alternative of synthetic drugs without side effects.

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Qualitative and Quantitative Analysis of Volatile Components of Zhengtian Pills Using Gas Chromatography Mass Spectrometry and Ultra-High Performance Liquid Chromatography

Cited by 7 publications

References 17 publications

The Antioxidative Action of ZTP by Increasing Nrf2/ARE Signal Pathway

The Antioxidative Action of ZTP by Increasing Nrf2/ARE Signal Pathway

Screening of potential phytocompounds from Euclea crispa (Thunb.) leaves targeting human epidermal growth factor receptor 2 (HER2) signaling pathway

Evaluation of Phytochemical Contents, Antimicrobial, and antioxidant Potential of Haloxylon Griffithii Collected From Northern Region of Balochistan, Pakistan

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