Quantification of oestrogen receptors in breast cancer: radiochemical assay on cytosols and cryostat sections compared with semiquantitative immunocytochemical analysis.

Scheres, H M; Goeij, Anton F. de; Rousch, Mat; Hondius, G.; Willebrand, D.; Gijzen, A.H.J.; Bosman, Fred T.

doi:10.1136/jcp.41.6.623

Cited by 25 publications

(11 citation statements)

References 25 publications

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“…The same scoring system reported by McCarty et al has been used by others (Scheres et al 1988;Bosman et al 1992). In the original scoring system there were five grades of staining intensity (0, 1 ϩ , 2 ϩ , 3 ϩ , and 4 ϩ ).…”

Section: Methodsmentioning

confidence: 99%

Temporal Changes in PTHrP, Bcl-2, Bax, Caspase, TGF-β, and FGF-2 Expression Following Growth Plate Irradiation With or Without Radioprotectant

Damron

Mathur

Horton

et al. 2004

J Histochem Cytochem.

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S U M M A R YThis study examined temporal changes in growth plate apoptosis molecules and growth factors in an animal model of radiation injury with and without a radioprotectant. Thirty weanling 5-week Sprague-Dawley rats underwent right knee irradiation with single-fraction 17.5 Gy while the left served as internal control. Six animals each were sacrificed at 0.5, 1, 2, 3, or 4 weeks after irradiation. Half of the animals received pretreatment with amifostine (WR-2721) radioprotectant. Immunohistochemical staining for PTHrP, Bcl-2, Bax, caspase-3, FGF-2, and TGF-␤ was performed. PTHrP decreased to a nadir at 1 week after irradiation but rebounded to above control levels at 2 weeks in the reserve and transitional zones. The radioprotectant amifostine blunted the decrease in PTHrP but kept PTHrP expression lower than controls during the rebound phase in untreated irradiated animals. Hypertrophic zone Bax expression was decreased by amifostine in both irradiated and non-irradiated limbs at 1 and 2 weeks. FGF, TGF-␤ , Bcl-2, and caspase levels generally decreased at 1 week and returned thereafter toward control levels. These findings underscore the importance of PTHrP in response to growth plate irradiation and show the novel finding of a decrease in Bax expression with amifostine pretreatment.

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Section: Methodsmentioning

confidence: 99%

Temporal Changes in PTHrP, Bcl-2, Bax, Caspase, TGF-β, and FGF-2 Expression Following Growth Plate Irradiation With or Without Radioprotectant

Damron

Mathur

Horton

et al. 2004

J Histochem Cytochem.

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show abstract

“…To evaluate the p53 immunohistochemical staining, we scored the percentage of positive cells (0-100%) and the intensity of the staining (weak, moderate, or intense) that was assessed semiquantitatively, as previously described. This evaluation method provided final rankings of values between 0 and 300 [31]. Moderate to strong staining intensity was easily identified at low magnification (40×), whereas weak staining was visible only at high magnification (400×).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Expression of the ribosomal proteins Rplp0, Rplp1, and Rplp2 in gynecologic tumors

et al. 2011

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“…Semiquantitative assessment of VEGF-C immunolabeling was performed by histoscore analysis (multiplication product of percentage of positive cells by staining intensity) (18). Percentage of positive cells was evaluated as follows: 0, Ͻ5% labeled cells; 1, 5-25% labeled cells; 2, 26 -50% labeled cells; 3, 51-75% labeled cells; and 4, Ͼ75% labeled cells.…”

Section: Discussionmentioning

confidence: 99%

Liver Metastases Arising from Well-Differentiated Pancreatic Endocrine Neoplasms Demonstrate Increased VEGF-C Expression

et al. 2003

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Quantification of oestrogen receptors in breast cancer: radiochemical assay on cytosols and cryostat sections compared with semiquantitative immunocytochemical analysis.

Cited by 25 publications

References 25 publications

Temporal Changes in PTHrP, Bcl-2, Bax, Caspase, TGF-β, and FGF-2 Expression Following Growth Plate Irradiation With or Without Radioprotectant

Temporal Changes in PTHrP, Bcl-2, Bax, Caspase, TGF-β, and FGF-2 Expression Following Growth Plate Irradiation With or Without Radioprotectant

Expression of the ribosomal proteins Rplp0, Rplp1, and Rplp2 in gynecologic tumors

Liver Metastases Arising from Well-Differentiated Pancreatic Endocrine Neoplasms Demonstrate Increased VEGF-C Expression

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