Quantification of β-carotene, retinol, retinyl acetate and retinyl palmitate in enriched fruit juices using dispersive liquid–liquid microextraction coupled to liquid chromatography with fluorescence detection and atmospheric pressure chemical ionization-mass spectrometry

Viñas, Pilar; Bravo-Bravo, María; López-Garcı́a, Ignacio; Hernández‐Córdoba, Manuel

doi:10.1016/j.chroma.2012.12.022

Cited by 33 publications

(7 citation statements)

References 31 publications

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“…The main qualitative and quantitative parameters (recovery, precision, LOD, type and volume of organic solvents, and analysis time) of the extraction procedure, described here, were compared with those of the previous DLLME methods developed to extract β-carotene from fruit juice samples (see Table ). − Actually, all selected methods show similar figures of merit with slight differences. Obviously, those based on MS detection (ref and this work) exhibit lower and very similar LODs.…”

Section: Resultsmentioning

confidence: 92%

“… − Actually, all selected methods show similar figures of merit with slight differences. Obviously, those based on MS detection (ref and this work) exhibit lower and very similar LODs. As far as the recovery is concerned, our method has a yield not as high as that of the ABLLME and AA-LDS-LLME-SFOD methods; however, the comparison is difficult to draw since the authors did not provide the approach applied for the calculation.…”

Section: Resultsmentioning

confidence: 92%

“…Considering the great importance assumed by green chemistry, nowadays, an analytical method should be evaluated also in terms of sustainability and not just for its cheapness, speediness, and figures of merit . From this perspective, liquid microextraction techniques possess all these characteristics and appear suitable for vitamin analysis, even if only few studies are present in the literature. − Among these, just two sustainable methods, combining DLLME with the use of an hydrophobic DES, were developed to isolate carotenoids from fruit juices. , In both studies, long-chain fatty compounds lacking in antioxidant activity were used as extracting solvents. In detail, Li et al.…”

Section: Introductionmentioning

confidence: 99%

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Hydrophobic Eutectic Solvent with Antioxidant Properties: Application for the Dispersive Liquid–Liquid Microextraction of Fat-Soluble Micronutrients from Fruit Juices

Bosco

Lisio

D’Angelo

et al. 2021

ACS Sustainable Chem. Eng.

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Despite the great interest devoted to eutectic solvents with significant negative deviations from ideality (namely, deep eutectic solvents), many hydrophobic liquid mixtures are ideal or quasi-ideal systems. In this regard, we propose the introduction of a hydrophobic eutectic solvent based on l-menthol and butylated hydroxytoluene, blended in a molar ratio of 3:1. The physicochemical characterization by means of differential scanning calorimetry and infrared spectroscopy has identified that it is an ideal eutectic solvent. Compared to other hydrophobic mixtures, this one represents an advanced solvent system due to its intrinsic antioxidant activity, which makes it an ideal green choice for the extraction and preservation of easily oxidizable lipophilic compounds. In order to evaluate its efficiency, it was applied for the first time as an extractant for the dispersive liquid–liquid microextraction of carotenoids and fat-soluble vitamins from fruit juices, carried out at 298 K. All extracts were analyzed by high-performance liquid chromatography–tandem mass spectrometry. The developed method was then validated, providing precise (4–8%) and accurate (4–6%) results on a commercial fruit juice containing declared values of β-carotene and α-tocopherol acetate. Recoveries were ≥70%, while the detection limits were 0.05 μg L–1 for β-carotene and 0.28 μg L–1 for α-tocopherol acetate. Here, we also propose an original approach to properly determine the antioxidant activity of eutectic mixtures since the available commercial kits cannot be applied to such systems without altering their nature. Last but not least, a circular process of reuse of this ideal eutectic solvent has been developed using a low-cost activated carbon material obtained from coconut shells.

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Section: Resultsmentioning

confidence: 92%

Section: Resultsmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hydrophobic Eutectic Solvent with Antioxidant Properties: Application for the Dispersive Liquid–Liquid Microextraction of Fat-Soluble Micronutrients from Fruit Juices

Bosco

Lisio

D’Angelo

et al. 2021

ACS Sustainable Chem. Eng.

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“…In food and beverages, natural antioxidants are progressively replacing their synthetic counterparts, that is, BHT and BHA [ 177 ]. β-Carotene, retinol, retinyl acetate, and retinyl palmitate have been determined in juices enriched with vitamins A and C, so-called ACE juices [ 178 ]. Rodriguez and Rodriguez-Amaya detected 10 CPs of β-carotene in processed acerola and mango juices and in dried apricots [ 40 ].…”

Section: Measurement Of Biological Samplesmentioning

confidence: 99%

Analytical tools for the analysis of β-carotene and its degradation products

Stutz

Bresgen

Eckl

2015

Free Radical Research

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Abstractβ-Carotene, the precursor of vitamin A, possesses pronounced radical scavenging properties. This has centered the attention on β-carotene dietary supplementation in healthcare as well as in the therapy of degenerative disorders and several cancer types. However, two intervention trials with β-carotene have revealed adverse effects on two proband groups, that is, cigarette smokers and asbestos-exposed workers. Beside other causative reasons, the detrimental effects observed have been related to the oxidation products of β-carotene. Their generation originates in the polyene structure of β-carotene that is beneficial for radical scavenging, but is also prone to oxidation. Depending on the dominant degradation mechanism, bond cleavage might occur either randomly or at defined positions of the conjugated electron system, resulting in a diversity of cleavage products (CPs).Due to their instability and hydrophobicity, the handling of standards and real samples containing β-carotene and related CPs requires preventive measures during specimen preparation, analyte extraction, and final analysis, to avoid artificial degradation and to preserve the initial analyte portfolio. This review critically discusses different preparation strategies of standards and treatment solutions, and also addresses their protection from oxidation. Additionally, in vitro oxidation strategies for the generation of oxidative model compounds are surveyed. Extraction methods are discussed for volatile and non-volatile CPs individually. Gas chromatography (GC), (ultra)high performance liquid chromatography (U)HPLC, and capillary electrochromatography (CEC) are reviewed as analytical tools for final analyte analysis. For identity confirmation of analytes, mass spectrometry (MS) is indispensable, and the appropriate ionization principles are comprehensively discussed. The final sections cover analysis of real samples and aspects of quality assurance, namely matrix effects and method validation.

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“…Numerous analytical approaches have been utilized/ reviewed for VA carotenoids analysis; these include flow-injection (FI)/flow based and manual colorimetry/ spectrophotometry (12)(13)(14), fluorimetry (15), voltam-metry (16), capillary electrophoresis and liquid chromatography coupled with different detectors (ultraviolet (17), fluorimetry (18), photodiode/diode array (19) and mass spectrometry (20)) in milk, biological fluids, pharmaceutical supplements, food, beverages and others. Although, these methods are accurate, selective, sensitive and coupled with good separation efficiency but have many drawbacks including lengthy and timeconsuming sample preparation procedures, low injection and sample throughputs, high-priced instrumentation and consumption of expensive and large amount of environmentally degrading reagents.…”

mentioning

confidence: 99%

A Flow Injection Chemiluminescence Method for the Determination of Retinol in Pharmaceutical Formulations by Using Luminol-Diperiodatoargentate(III) Reaction

Ambar

Ameen

AlNadhari

et al. 2020

J Nutr Sci Vitaminol

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In this paper, a chemiluminescence (CL) method is proposed for retinol determination by combining flow injection (FI) methodology. The CL reaction is based on the oxidation of luminol by diperiodatoargentate(III) (DPA) in the presence of retinol. Under the optimum conditions, the relative CL intensity was linear to the concentration of retinol over the range 5.0310 23 -14 mg L 21 (y5347.26x12.5944, R 2 50.9999, n58) with limit of detection (LOD) of 1.5310 23 mg L 21 (S/N53) and limit of quantification (LOQ) of 5.0310 23 mg L 21 (S/N510). The relative standard deviation (RSD) was from 1.04-3.4% over the range studied and injection throughputs of 150 h 21 . The method was satisfactorily applied to retinol in pharmaceutical formulation samples. The samples were saponified and extracted with liquid-liquid extraction using ether as an extractant. The possible CL mechanism is supported by CL and UV-visible spectrophotometric studies.

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Quantification of β-carotene, retinol, retinyl acetate and retinyl palmitate in enriched fruit juices using dispersive liquid–liquid microextraction coupled to liquid chromatography with fluorescence detection and atmospheric pressure chemical ionization-mass spectrometry

Cited by 33 publications

References 31 publications

Hydrophobic Eutectic Solvent with Antioxidant Properties: Application for the Dispersive Liquid–Liquid Microextraction of Fat-Soluble Micronutrients from Fruit Juices

Hydrophobic Eutectic Solvent with Antioxidant Properties: Application for the Dispersive Liquid–Liquid Microextraction of Fat-Soluble Micronutrients from Fruit Juices

Analytical tools for the analysis of β-carotene and its degradation products

A Flow Injection Chemiluminescence Method for the Determination of Retinol in Pharmaceutical Formulations by Using Luminol-Diperiodatoargentate(III) Reaction

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