Quantitative analysis of Pentalgin N tablets by gradient and isocratic high-performance liquid chromatography

Голубицкий, Г. Б.; Будко, Е. В.; Иванов, В. М.

doi:10.1134/s106193480601014x

Cited by 5 publications

(4 citation statements)

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“…As is well-documented in the references, MTZ is a pro-drug that hydrolyses quickly to 4-MAA (7,8,14,19). This transformation occurs both in water and methanol, being faster in water and at acidic pH values (14).…”

Section: Development and Validation Of The Hplc Methodsmentioning

confidence: 77%

“…In fact, the 4-MAA is the form in which MTZ acts because it is not enzimatically completely hydrolyzed 5 min after oral ingestion (34). It was then necessary to add Na 2 SO 3 to all the solutions containing MTZ (stock standard solutions and working standard solutions) (19) and to the mobile phase, to prevent the hydrolysis of MTZ, because tests showed that if no sodium sulphite was added when MTZ was present, the MTZ hydrolysis to 4-MAA was produced even during the chromatographic process. A small peak assigned to 4-MAA was placed in the chromatogram between peaks of MTZ and TMD at around 5 min ( Figure 2) when Na 2 SO 3 was added to the standard samples but not to the mobile phase.…”

Section: Development and Validation Of The Hplc Methodsmentioning

confidence: 99%

“…A few methods claim to determine MTZ by HPLC (17,18), but without taking into account the spontaneous hydrolysis process of MTZ to 4-MAA. When MTZ was determined in Pentalgin tablets by HPLC, the use of sodium sulphite was proposed to prevent the MTZ hydrolysis process (19). Recently, a new kinetic-chromatographic determination of the hydrolysis product of MTZ (4-MAA) in pharmaceutical commercial products has been reported (20).…”

Section: Introductionmentioning

confidence: 99%

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Determination of Tramadol, Metamizole, Ropivacaine, and Bupivacaine in Analgesic Mixture Samples by HPLC with DAD Detection

Salmerón-García

Navas

Martin

et al. 2009

Journal of Chromatographic Science

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A high-performance liquid chromatography-diode array detection method was developed and validated to simultaneously determine tramadol (TMD), metamizole (MTZ), ropivacaine (RPV), and bupivacaine (BPV) in the presence of 4-methylaminoantypirine (4-MAA), the metabolite of MTZ, in analgesic mixtures samples used in Patient Controlled Analgesia (PCA). Chromatographic separation is achieved with a C-18 column using a mixture of ACN-methanol-water adjusted to pH 3.0 with NaH(2)PO(4) 0.05M (10:25:65 v/v) in an isocratic mobile phase at a flow rate of 0.8 mL/min. 0.5 mg/mL of Na(2)SO(3) in the water of the mobile phase was necessary to prevent the fast MTZ hydrolysis process to 4-MAA. Ultraviolet-diode array detection was used and chromatograms were registered at the wavelength of 230 nm. The method was linear in the range of 2.2-80.0 mg/L for TMD, 4.1-140.0 mg/L for MTZ, 2.3-40.0 mg/L for RPV, and 2.9-40.0 mg/L for BPV. Validation of the method was made in terms of accuracy, intra- and interday precision, as well as quantification and detection limits. The hydrolysis of MTZ to 4-MAA was studied and verified by mass spectrometry. The developed method was used successfully to evaluate the chemical stability of binary analgesic TMD mixtures with MTZ, RPV, or BPV. The mixtures were tested at standard concentrations used in PCA and in different storage conditions. When mixtures contained MTZ, a chromatographic peak from the metabolite 4-MAA was always detected in the chromatograms.

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Section: Development and Validation Of The Hplc Methodsmentioning

confidence: 77%

Section: Development and Validation Of The Hplc Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of Tramadol, Metamizole, Ropivacaine, and Bupivacaine in Analgesic Mixture Samples by HPLC with DAD Detection

Salmerón-García

Navas

Martin

et al. 2009

Journal of Chromatographic Science

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show abstract

“…Judging by the methods of analysis of medicinal substances based on the materials of the original articles, we can say that chromatographic methods of analysis are still in the lead. Electrochemical methods for the analysis of medicinal substances by the number of publications rank third, behind photometry and spectrophotometry, with ∼20% of works devoted to the electrochemical determination of medicinal substances [14][15][16][17][18][19][20][21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

The Ionic Associate of Metamizole as an Electrode-Active Component of a PVC Plasticized Membrane Electrode

et al. 2022

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The technology for manufacturing a film membrane of the metamizole-selective electrode containing ion associate metamizole-octadecylammonium ODAH+MT− as an electrode active component (EAC) has been proposed. The main potentiometric characteristics of the metamizole-selective electrode have been determined. The expediency of the proposed design of the metamizole selective electrode for the determination of metamizole in dosage forms has been substantiated. The best composition of the membrane (wt.%) of the metamizole-selective electrode has corresponded to: ODAH+MT−—5.3; 2-nitrophenyloctylether—63.1; poly(vinyl chloride)—31.6. Electrode-active component in the membrane phase functions as an ion associate ODAH+MT−. Potentiometric characteristics of metamizole-selective electrode have been determined, which corresponded to: linear range 1 × 10−2–1 × 10−4 with limit of detection 4.58 × 10−5 M, electrode function slope −48.5 mV/dec., working interval pH 4.5–7.3, response time 60 s. The potentiometric coefficients of selectivity of the metamizole-selective electrode with respect to various ions have been determined. The possibility of determining metamizole in a medicinal product has been tested. The results of the analyses show good agreement between the two methods (relative error less than 7.0%) with coefficients of variation less than 5% for MT-SE and iodometric methods.

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HPLC–DAD Method for Simultaneous Determination of Dipyrone (Metamizole) and Caffeine in Tablets and Identification of Major Degradation Product by Direct Infusion ESI–MS

et al. 2017

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Quantitative analysis of Pentalgin N tablets by gradient and isocratic high-performance liquid chromatography

Cited by 5 publications

References 0 publications

Determination of Tramadol, Metamizole, Ropivacaine, and Bupivacaine in Analgesic Mixture Samples by HPLC with DAD Detection

Determination of Tramadol, Metamizole, Ropivacaine, and Bupivacaine in Analgesic Mixture Samples by HPLC with DAD Detection

The Ionic Associate of Metamizole as an Electrode-Active Component of a PVC Plasticized Membrane Electrode

HPLC–DAD Method for Simultaneous Determination of Dipyrone (Metamizole) and Caffeine in Tablets and Identification of Major Degradation Product by Direct Infusion ESI–MS

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