Quantitative Determination of Amino Acids on Filter Paper Chromatograms by Direct Photometry

McFarren, Earl F.; Mills, John A.

doi:10.1021/ac60064a010

Cited by 130 publications

(17 citation statements)

References 33 publications

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“…Protein solutions in 500 vol. of 6 N-HCI were hydrolysed in sealed glass tubes at 110°for 24 h. The acid hydrolysates were evaporated to dryness under vacuum and the last traces of HCI removed by the method of McFarren & Mills (1952).…”

Section: Methodsmentioning

confidence: 99%

Rates of Absorption by rat Intestine of Pancreatic Hydrolysates of Proteins and Their Corresponding Amino Acid Mixtures

et al. 1971

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1. Though the occurrence of intestinal mucosal uptake of intact peptides, with cellular hydrolysis to amino acids, has been established, the importance of this mode of absorption in protein absorption is not known. This paper describes a comparison of the rates of intestinal absorption of pancreatic hydrolysates of four proteins with . those of the corresponding acid hydrolysates or amino acid mixtures.2. The results show that the absorption of pancreatic hydrolysates, consisting largely of small peptides of two to six amino acid residues, is substantially more rapid than that of the corresponding mixtures of free amino acids. This shows that the small intestine has a high capacity for absorption from mixtures of small peptides such as might be produced during protein digestion, and supports the hypothesis that mucosal uptake of intact oligopeptides is an important mode of protein absorption.Though the existence of a second mode of protein absorption, mucosal uptake of oligopeptides with cellular hydrolysis, as distinct from complete intralumen hydrolysis with uptake of free amino acids, is established (Newey indicate that the dipeptide uptake mechanism remains intact and in these circumstances peptide uptake is nutritionally important. Features of the intestinal transport of individual peptides, including the faster absorption of amino acids from high concentrations of small peptides than from the equivalent

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Section: Methodsmentioning

confidence: 99%

Rates of Absorption by rat Intestine of Pancreatic Hydrolysates of Proteins and Their Corresponding Amino Acid Mixtures

et al. 1971

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“…The following fractions resulted from application of this method to the apples: CCU washes, used to extract waxes from an acidified aqueous methanol solution of the dodecylguanidine 3. Aqueous discard, left after extraction of the dodecylguamdine-bromocresol purple complex from aqueous methanol into 4. CHC13 washes 5.…”

Section: Methodsmentioning

confidence: 99%

Plant Metabolism of Fungicides, Translocation and Metabolism of Dodecylguanidine Acetate (Dodine) Fungicide in Apple Trees, Using C¹⁴ Radiotagged Dodine

Curry¹

1962

J. Agric. Food Chem.

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The leaves along two branches of a bearing apple tree were painted repeatedly throughout the growing season with a solution of dodine tagged with C14 on the guanidine carbon. Radiocounting and chemical analysis of apples harvested from these branches indicated that there is no translocation of dodine from foliage into the fruits of the tree. The degradation products of dodine are translocated only very slightly and appear to be simple amino acidand guanidine-type moieties bound in proteins and peptides.

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“…The quantitative procedure has been published in detail (13). The following modifications, which do not alter the separations, are incorporated in this present study.…”

Section: Methodsmentioning

confidence: 99%

Accuracy of Quantitative Paper Chromatography in Amino Acid Determination Using Direct Photometry

Roberts¹,

Kolor²

1957

Anal. Chem.

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Quantitative Determination of Amino Acids on Filter Paper Chromatograms by Direct Photometry

Cited by 130 publications

References 33 publications

Rates of Absorption by rat Intestine of Pancreatic Hydrolysates of Proteins and Their Corresponding Amino Acid Mixtures

Rates of Absorption by rat Intestine of Pancreatic Hydrolysates of Proteins and Their Corresponding Amino Acid Mixtures

Plant Metabolism of Fungicides, Translocation and Metabolism of Dodecylguanidine Acetate (Dodine) Fungicide in Apple Trees, Using C¹⁴ Radiotagged Dodine

Accuracy of Quantitative Paper Chromatography in Amino Acid Determination Using Direct Photometry

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Quantitative Determination of Amino Acids on Filter Paper Chromatograms by Direct Photometry

Cited by 130 publications

References 33 publications

Rates of Absorption by rat Intestine of Pancreatic Hydrolysates of Proteins and Their Corresponding Amino Acid Mixtures

Rates of Absorption by rat Intestine of Pancreatic Hydrolysates of Proteins and Their Corresponding Amino Acid Mixtures

Plant Metabolism of Fungicides, Translocation and Metabolism of Dodecylguanidine Acetate (Dodine) Fungicide in Apple Trees, Using C14 Radiotagged Dodine

Accuracy of Quantitative Paper Chromatography in Amino Acid Determination Using Direct Photometry

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Plant Metabolism of Fungicides, Translocation and Metabolism of Dodecylguanidine Acetate (Dodine) Fungicide in Apple Trees, Using C¹⁴ Radiotagged Dodine