Quantum Dot Biosensors for Ultrasensitive Multiplexed Diagnostics

Geißler, Daniel; Charbonnière, Loı̈c J.; Ziessel, Raymond; Butlin, Nathaniel G.; Löhmannsröben, Hans‐Gerd; Hildebrandt, Niko

doi:10.1002/anie.200906399

Cited by 271 publications

(237 citation statements)

References 41 publications

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“…The solution to this challenge has been to pair QDs as FRET acceptors with luminescent lanthanide complexes as donors. 12 -8 -10 -7 s for QDs). As such, directly excited QDs return to their ground state and become good acceptors following a microsecond delay after flash/pulsed excitation, whereas lanthanide ions remain in their excited state as good donors.…”

Section: Bioanalysis and Bioimaging With Quantum Dotsmentioning

confidence: 99%

Quantum Dots in Bioanalysis: A Review of Applications across Various Platforms for Fluorescence Spectroscopy and Imaging

2013

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Semiconductor quantum dots (QDs) are brightly luminescent nanoparticles that have found numerous applications in bioanalysis and bioimaging. In this review, we highlight recent developments in these areas in the context of specific methods for fluorescence spectroscopy and imaging. Following a primer on the structure, properties, and biofunctionalization of QDs, we describe select examples of how QDs have been used in combination with steady-state or time-resolved spectroscopic techniques to develop a variety of assays, bioprobes, and biosensors that function via changes in QD photoluminescence intensity, polarization, or lifetime. Some special attention is paid to the use of Förster resonance energy transfer-type methods in bioanalysis, including those based on bioluminescence and chemiluminescence. Direct chemiluminescence, electrochemiluminescence, and charge transfer quenching are similarly discussed. We further describe the combination of QDs and flow cytometry, including traditional cellular analyses and spectrally encoded barcode-based assay technologies, before turning our attention to enhanced fluorescence techniques based on photonic crystals or plasmon coupling. Finally, we survey the use of QDs across different platforms for biological fluorescence imaging, including epifluorescence, confocal, and twophoton excitation microscopy; single particle tracking and fluorescence correlation spectroscopy; super-resolution imaging; near-field scanning optical microscopy; and fluorescence lifetime imaging microscopy. In each of the above-mentioned platforms, QDs provide the brightness needed for highly sensitive detection, the photostability needed for tracking dynamic processes, or the multiplexing capacity needed to elucidate complex systems. There is a clear synergy between advances in QD materials and spectroscopy and imaging techniques, as both must be applied in concert to achieve their full potential.

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Section: Bioanalysis and Bioimaging With Quantum Dotsmentioning

confidence: 99%

Quantum Dots in Bioanalysis: A Review of Applications across Various Platforms for Fluorescence Spectroscopy and Imaging

2013

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“…84 These characteristics make them very suitable for the POC diagnosis of infectious disease because of their simple, low-cost, and robust properties. 85 As shown in Figure 5, this lateral flow system-based paper POCT platform was composed of an absorbent pad (wick), nitrocellulose membrane, conjugate pad (CP), and sample pad. The triangular plate-shaped silver nanoparticles conjugated with different antibodies, which could result in easily distinguishable colors due to the size-tunable absorption spectra of silver nanoparticles, were preloaded in the CP.…”

Section: Paper-based Poct Platformmentioning

confidence: 99%

Application of nanodiagnostics in point-of-care tests for infectious diseases

Wang¹,

Yu²,

Kong³

et al. 2017

IJN

103

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Although tremendous efforts have been put into the treatment of infectious diseases to prevent epidemics and mortality, it is still one of the major health care issues that have a profound impact on humankind. Therefore, the development of specific, sensitive, accurate, rapid, low-cost, and easy-to-use diagnostic tools is still in urgent demand. Nanodiagnostics, defined as the application of nanotechnology to medical diagnostics, can offer many unique opportunities for more successful and efficient diagnosis and treatment for infectious diseases. In this review, we provide an overview of the nanodiagnostics for infectious diseases from nanoparticle-based, nanodevice-based, and point-of-care test (POCT) platforms. Most importantly, emphasis focused on the recent trends in the nanotechnology-based POCT system. The current state-of-the-art and most promising point-of-care nanodiagnostic technologies, including miniaturized diagnostic magnetic resonance platform, magnetic barcode assay system, cell phone-based polarized light microscopy platform, cell phone-based dongle platform, and paper-based POCT platform, for infectious diseases were fully examined. The limitations, challenges, and future trends of the nanodiagnostics in POCTs for infectious diseases are also discussed.

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“…Fluorescence spectroscopy is one of the most common methods in detecting biomolecules [1][2][3][4][5][6][7]. In this process biomolecules are labeled with a fluoroprobe, which can be excited with a short pulse of ultraviolet (UV) light and re-emit radiation in visible spectral range.…”

Section: Introductionmentioning

confidence: 99%

Optimization of a Dielectric Barrier Discharge for Pulsed UV Emission of XeCl at 308 nm

Pipa

Bussiahn

2011

Contrib. Plasma Phys.

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A pulsed dielectric barrier discharge (DBD) has been investigated in a wide range of experimental conditions with the purpose of optimization for XeCl excimer radiation. For that the following operation parameters had been considered: four different lamps of coaxial geometry with gas gaps in the range of 1.3 -6.5 mm; gas mixtures of xenon and chlorine containing admixtures of 1%, 2% and 4% Cl2 at total filling pressures between 5 mbar and 600 mbar; voltage rise times of 20 -50 ns and voltage amplitudes of up to 12 kV. A maximum radiation pulse energy of 1.8 μJ has been detected at 310 ± 10 nm with an estimated radiation decay by three orders of magnitude within about 5 μs. It was shown that the minimization of the voltage rise time is essential for enhancing the radiation pulse energy. Furthermore a correlation between the discharge geometry and the optimum pressure for maximum radiation output was observed. The decay characteristics of the excimer emission provides evidence of the harpoon reaction being the main channel of XeCl formation under our operation conditions.

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Quantum Dot Biosensors for Ultrasensitive Multiplexed Diagnostics

Cited by 271 publications

References 41 publications

Quantum Dots in Bioanalysis: A Review of Applications across Various Platforms for Fluorescence Spectroscopy and Imaging

Quantum Dots in Bioanalysis: A Review of Applications across Various Platforms for Fluorescence Spectroscopy and Imaging

Application of nanodiagnostics in point-of-care tests for infectious diseases

Optimization of a Dielectric Barrier Discharge for Pulsed UV Emission of XeCl at 308 nm

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