Quantum dots-based hydrogel microspheres for visual determination of lactate and simultaneous detection coupled with microfluidic device

Yang, Sisi; Fan, Li; Liu, Yucheng; Ning, Yu; Songbai, Tian; Zuo, Pengfei; Ji, Xinghu; He, Zhike

doi:10.1016/j.microc.2021.106801

Cited by 18 publications

(10 citation statements)

References 46 publications

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“…The relative error values are greater when LOx/γ-/α-Fe 2 O 3 was used compared to those values obtained with LOx/γ-/α-Fe 2 O 3 /ZnO. Finally, the obtained values of lactate concentration obtained by using the NPs synthesized in this work are comparable with those previously obtained by other authors in body fluids such as sweat [ 52 , 53 ] and blood [ 49 , 54 , 55 ]. A better fit in the linearity of the lactate sensor is important because it reduces measurement errors and is easier to calibrate.…”

Section: Resultssupporting

confidence: 84%

Comparative Colorimetric Sensor Based on Bi-Phase γ-/α-Fe2O3 and γ-/α-Fe2O3/ZnO Nanoparticles for Lactate Detection

et al. 2022

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This work reports on Fe2O3 and ZnO materials for lactate quantification. In the synthesis, the bi-phase γ-/α-Fe2O3 and γ-/α-Fe2O3/ZnO nanoparticles (NPs) were obtained for their application in a lactate colorimetric sensor. The crystalline phases of the NPs were analyzed by XRD and XPS techniques. S/TEM images showed spheres with an 18 nm average and a needle length from 125 to 330 nm and 18 nm in diameter. The γ-/α-Fe2O3 and γ-/α-Fe2O3/ZnO were used to evaluate the catalytic activity of peroxidase with the substrate 3,3,5,5-tetramethylbenzidine (TMB), obtaining a linear range of 50 to 1000 μM for both NPs, and a 4.3 μM and 9.4 μM limit of detection (LOD), respectively. Moreover, γ-/α-Fe2O3 and γ-/α-Fe2O3/ZnO/lactate oxidase with TMB assays in the presence of lactate showed a linear range of 50 to 1000 µM, and both NPs proved to be highly selective in the presence of interferents. Finally, a sample of human serum was also tested, and the results were compared with a commercial lactometer. The use of ZnO with Fe2O3 achieved a greater response toward lactate oxidation reaction, and has implementation in a lactate colorimetric sensor using materials that are economically accessible and easy to synthesize.

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Section: Resultssupporting

confidence: 84%

Comparative Colorimetric Sensor Based on Bi-Phase γ-/α-Fe2O3 and γ-/α-Fe2O3/ZnO Nanoparticles for Lactate Detection

et al. 2022

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“…To develop sensing applications, a facile enzymatic FL bio-probe of lactate was constructed based on Co@BQDs. As shown in Scheme b, lactate is added into the PBS dispersion of Co@BQDs containing lactate oxidase (LOx), which causes enzymatic oxidization of lactate by LOx to generate pyruvate and H 2 O 2 . , Then, H 2 O 2 -triggered oxidation of boron atoms leads to FL quenching effects of Co@BQDs . With an increase of lactate concentration ( C lactate ), FL spectra of PBS dispersions with “Co@BQDs plus LOx” display a gradual decrease in FL emission intensities with negligible peak-shape changes (Figure a).…”

Section: Resultsmentioning

confidence: 99%

Metal-Doped Boron Quantum Dots for Versatile Detection of Lactate and Fluorescence Bioimaging

Yang

Jin

Gui

2022

ACS Appl. Mater. Interfaces

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To improve the stability and fluorescence (FL) of monoelemental boron nanomaterials, this work put forward a metal-coordination strategy to explore emerging metal-doped boron quantum dots, Co@BQDs. Through theoretical calculations, B−Co bonding as predicted can suppress the B−O reaction and protect the electronic structures of exfoliated twodimensional (2D) boron from oxidation and decomposition upon exposure to oxygen. In experimental studies, Co 2+ was added into a dispersion liquid of bulk boron and subjected to probe sonication to promote Co 2+ adsorption on the surface of exfoliated 2D boron, followed by Co 2+ coordination with exposed boron atoms. Solvothermal treatment of exfoliated 2D boron resulted in the generation of Co 2+ -doped 0D boron Co@BQDs. Experimental results confirm that Co@BQDs have higher colloidal and FL stability than BQDs as a reference. B−Co bonding formation to suppress the B−O reaction ensures the high stability of exfoliated boron structures. A dispersion liquid of Co@BQDs with stable and bright FL was used for visual FL imaging of solutions and solid substrates. Based on enzymatic and cascade oxidation-induced FL quenching of Co@BQDs, a novel FL bio-probe of lactate was explored. This bio-probe, with a broad detection range of 0.01−10 mM and a low detection limit of 3.1 μM, enables FL sensing of lactate in biosamples and shows high detection recoveries of 98.0−102.8%. Moreover, this bio-probe realized versatile FL imaging and visual detection of lactate in liquid/solid-phase systems. These results demonstrate great prospects of Co@BQDs as emerging and efficient imaging reagents for long-term tracking and bioimaging applications.

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“…Phenyl-doped g-C 3 N 4 (PDCN) nanosheets were fabricated according to the methods in the literature [ 28 ]. Alg@PDCN@QDs-LOx MSs were synthesized in the following procedures [ 29 ]. Firstly, a solution of 1% sodium alginate was obtained by dissolving sodium alginate in 20 mM pH 8.2 tris solution and stirring at about 25 °C for 20 min until the sodium alginate completely dissolved.…”

Section: Methodsmentioning

confidence: 99%

Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate

et al. 2022

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Since it is difficult for human eyes to distinguish between two identical colors with only <15% variation in brightness, mono-color fluorescent hydrogel microspheres have some limitations in the detection of lactate. Herein, we prepared novel dual-color fluorescent hydrogel microspheres, which can achieve hue transformation. Microspheres were prepared by introducing a fluorescent nanoparticle as the reference signal while CdTe QDs were used as the response signal. We used smartphones with image processing software to collect and analyze data. In this way, the signal of lactate was converted to RGB (red, green, and blue) values, which can be quantitatively read. Within 10 to 1500 μM, the R/G values of the microspheres had a linear relationship with the logarithm of the lactate concentration. Moreover, color cards for lactate detection were prepared, from which the color change and concentration of lactate could be easily read by the naked eye. It is worth mentioning that this method was successfully applied to screen patients with hyperlactatemia.

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Quantum dots-based hydrogel microspheres for visual determination of lactate and simultaneous detection coupled with microfluidic device

Cited by 18 publications

References 46 publications

Comparative Colorimetric Sensor Based on Bi-Phase γ-/α-Fe2O3 and γ-/α-Fe2O3/ZnO Nanoparticles for Lactate Detection

Comparative Colorimetric Sensor Based on Bi-Phase γ-/α-Fe2O3 and γ-/α-Fe2O3/ZnO Nanoparticles for Lactate Detection

Metal-Doped Boron Quantum Dots for Versatile Detection of Lactate and Fluorescence Bioimaging

Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate

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