Quercetin inhibits human breast cancer cell proliferation and induces apoptosis via Bcl-2 and Bax regulation

Zhang, Li

doi:10.3892/mmr.2012.845

Cited by 82 publications

(38 citation statements)

References 14 publications

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“…One of the major determinants of cell survival is the balance between the anti-apoptotic and pro-apoptotic members of the Bcl-2 family [18]. In the Bcl-2 protein family, pro-apoptotic factor Bax and anti-apoptotic factor Bcl-2 are the active regulators and effectors, and the ratio between Bcl-2 and Bax (Bcl-2/Bax) affects apoptosis induction [19,20]. In the meantime, we evaluated Bcl-2 and Bax after HMGB3 knockdown and found that Bax increased and Bcl-2 reduced when compared with the control group.…”

Section: Discussionmentioning

confidence: 99%

Knockdown of High Mobility Group-Box 3 (HMGB3) Expression Inhibits Proliferation, Reduces Migration, and Affects Chemosensitivity in Gastric Cancer Cells

et al. 2016

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BackgroundHigh mobility group-box 3 (HMGB3) has been shown to affect tumor initiation and progression. This research aimed to investigate the role of HMGB3 in gastric cancer (GC) cell proliferation, migration, invasion, chemoresistance, and its potential molecular mechanisms.Material/MethodsGC MGC803 and BGC823 cells were transfected with siRNA targeting the HMGB3 gene. The expressions of HMGB3 protein in MGC803 and BGC823 cells after transfection were detected by Western blot assays. We detected cell proliferation and cell cycle by MTT and flow cytometry assay. Cell migration and invasion were determined by wound scratch and transwell assay. MGC803 and BGC823 cells were treated with various concentrations of oxaliplatin, cisplatin, and paclitaxel. After 24 hours of drug exposure, we performed MTT assays to investigate chemoresistance in both groups. Western blot assays were used to detect related proteins expression.ResultsSilencing of HMGB3 inhibited cell proliferation and induced G0/G1 phase arrest of GC cells partly via modulating p53 and p21 pathways, and downregulating Bcl-2/Bax ratio. RNA interference of HMGB3 inhibited cell invasion and migration by downregulating MMP2 and MMP9. Silencing of HMGB3 enhanced sensitive to cisplatin and paclitaxel, and reduced sensitive to oxaliplatin.ConclusionsThese findings suggest the importance of HMGB3 in the regulation of growth, migration, and apoptosis of GC, improve our understanding of the mechanisms of GC pathogenesis, and may promote the development of novel targeted therapies.

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Section: Discussionmentioning

confidence: 99%

Knockdown of High Mobility Group-Box 3 (HMGB3) Expression Inhibits Proliferation, Reduces Migration, and Affects Chemosensitivity in Gastric Cancer Cells

et al. 2016

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“…The exposure of MCF-7 breast cancer cells to quercetin (50–200 μM) caused a dose- and time-dependent decrease of proliferation through induction of apoptosis, which was accompanied by up-regulation of Bax and down-regulation of Bcl-2 [100]. The inhibition of insulin receptor signaling by quercetin (100 μM) also impairs proliferation of MDA-MB-231 breast cancer cells.…”

Section: Experimental Studiesmentioning

confidence: 99%

Natural Polyphenols for Prevention and Treatment of Cancer

et al. 2016

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There is much epidemiological evidence that a diet rich in fruits and vegetables could lower the risk of certain cancers. The effect has been attributed, in part, to natural polyphenols. Besides, numerous studies have demonstrated that natural polyphenols could be used for the prevention and treatment of cancer. Potential mechanisms included antioxidant, anti-inflammation as well as the modulation of multiple molecular events involved in carcinogenesis. The current review summarized the anticancer efficacy of major polyphenol classes (flavonoids, phenolic acids, lignans and stilbenes) and discussed the potential mechanisms of action, which were based on epidemiological, in vitro, in vivo and clinical studies within the past five years.

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“…Morbidity and mortality are high in female malignant tumors (1). According to the World Health Organization, ~1.3 million women are diagnosed with breast cancer each year (2). The incidence rate of breast cancer ranks first among all malignant tumors in women and breast cancer mortality is only lower than that of lung cancer (3,4).…”

Section: Introductionmentioning

confidence: 99%

Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro

Deng

Song

Yingfeng

et al. 2013

Experimental and Therapeutic Medicine

111

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Quercetin is a hydrophobic agent with potential anticancer activity. The aim of the present study was to observe the effects of quercetin on the proliferation of the breast cancer cell line MCF-7 and the gene expression of survivin. The molecular mechanism underlying the antiproliferative effect of quercetin was also investigated. MCF-7 breast cancer cells were treated with various concentrations of quercetin. The inhibitory effect of quercetin on proliferation was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method and the inhibition rate was calculated. Cellular apoptosis was detected by immunocytochemistry and survivin mRNA expression levels were observed using reverse transcription-polymerase chain reaction (RT-PCR). Western blot analysis was used to analyze changes in the expression levels of survivin protein. Quercetin induced the apoptosis of MCF-7 cells and inhibited the proliferation of the MCF-7 breast cancer cells in a time- and concentration-dependent manner. The mRNA and protein expression levels of survivin were reduced as the concentration of quercetin increased. Quercetin inhibited the growth of MCF-7 cells and promoted apoptosis by inducing G0/ G1 phase arrest. It also regulated the expression of survivin mRNA in MCF-7 cells, which may be the mechanism underlying its antitumor effect.

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Quercetin inhibits human breast cancer cell proliferation and induces apoptosis via Bcl-2 and Bax regulation

Cited by 82 publications

References 14 publications

Knockdown of High Mobility Group-Box 3 (HMGB3) Expression Inhibits Proliferation, Reduces Migration, and Affects Chemosensitivity in Gastric Cancer Cells

Knockdown of High Mobility Group-Box 3 (HMGB3) Expression Inhibits Proliferation, Reduces Migration, and Affects Chemosensitivity in Gastric Cancer Cells

Natural Polyphenols for Prevention and Treatment of Cancer

Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro

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