Radiation-induced modification of the chromosome sensitivity of human somatic cells to the testing mutagenic exposure of bleomycin in vitro

Pilinskaya, M.; Dybskiy, S. S.; Dybskaya, Ye. B.; Pedan, L.

doi:10.3103/s0095452710020088

Cited by 4 publications

(5 citation statements)

References 8 publications

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“…Statistical calculations were performed on a PC. The coefficient of hidden chromosomal instability (Chci) was calculated to reveal individuals with hypersensitivity to the action of mitomycin C [15]:…”

Section: Methodsmentioning

confidence: 99%

“…According to Pilinskaya (2010), an induced cytogenetic effect exceeds the average group level of chromosomal aberrations in hypersensitive persons, so Chci in them will always be > 1. The Student's t test was used to reveal the significance of differences between the compared parameters [2].…”

Section: Methodsmentioning

confidence: 99%

“…To determine an individual hypersensitivity of a human being to the influence of mutagenic factors an additional mutagenic load on the PBL in vitro is used in the G2-phase of the cell cycle [16]. This method enables the authors to estimate a potential possibility of the genomic instability occurrence, when an organism gets in unfavorable environmental conditions [15]. Ionizing radiation is commonly used as a mutagen-provocateur, but over the last years specialists began to use chemical mutagens, and most of all bleomycin, antitumor antibiotic, which, being a radiomimetic, is able to induce a strong cytogenetic effect in the PBL in vitro [8,15].…”

Section: Introductionmentioning

confidence: 99%

“…This method enables the authors to estimate a potential possibility of the genomic instability occurrence, when an organism gets in unfavorable environmental conditions [15]. Ionizing radiation is commonly used as a mutagen-provocateur, but over the last years specialists began to use chemical mutagens, and most of all bleomycin, antitumor antibiotic, which, being a radiomimetic, is able to induce a strong cytogenetic effect in the PBL in vitro [8,15]. Some other mutagens, namely: dimatif [14], dimethoate, an insecticide, and mitomycin C, an antitumor antibiotic are used along with bleomycin to determine the sensitivity of the chromosomes to the effects of the chemical mutagenic load in vitro [4,8].…”

Section: Introductionmentioning

confidence: 99%

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Individual Sensitivity of the Peripheral Blood Lymphocytes to the in Vitro Action of Mitomycin C in Children with Juvenile Rheumatoid Arthritis

2016

Russian Journal of Biological Research

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The study was designed to estimate the levels of spontaneous and induced chromosome aberrations in the peripheral blood lymphocytes (PBL) of patients with juvenile rheumatoid arthritis (JRA) and their healthy peers. An increased spontaneous level of chromosomal aberrations has been found in children and adolescents with JRA in comparison with healthy agemates. It has been established that the effect of the mitomycin C model mutagen on the PBL in vitro causes a multiple increase in the levels of chromosomal aberrations in both groups under investigation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Individual Sensitivity of the Peripheral Blood Lymphocytes to the in Vitro Action of Mitomycin C in Children with Juvenile Rheumatoid Arthritis

2016

Russian Journal of Biological Research

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“…In our lab purposeful research of radiation-induced untargeted cytogenetic effects was conducted in 2005-2015 in the following main areas [14][15][16][17].…”

Section: Lecturementioning

confidence: 99%

Chromosomal Mutagenesis in Human Somatic Cells: 30-Year Cytogenetic Monitoring After Chornobyl Accident

Pilinska¹,

Shemetun²,

Shemetun³

et al. 2016

Exp. Onc.

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In the lecture we have generalized and analyzed the data of cytogenetic laboratory of National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine on 30-year selective cytogenetic monitoring among the priority contingents of different ages exposed to radiation after Chornobyl accident in Ukraine. It is highlighted that not only targeted but also untargeted radiation-induced cytogenetic effects should be explored, especially in delayed terms following radiation exposure. The new methodical approaches for studying “bystander effect”, individual radiosensitivity, and various forms of radiation-induced chromosomal instability (delayed, hidden, transmissible) have been proposed. These approaches proved to be advantageous for analyzing cytogenetic patterns of induction and persistence of chromosomal instability in human somatic cells because of “bystander effect” and “bystander type effect”. The phenomenon of positive “reverse” bystander effect has been found. The possibility of modifying the inherited individual human susceptibility to mutagenic exposure by ionizing radiation has been estimated. Finally, the association between hypersensitivity to radiation exposure and realization of oncopathology in exposed individuals has been revealed. The increased intensity of human somatic chromosomal mutagenesis was confirmed not only in the nearest but in the delayed terms following Chornobyl accident as a result of radiation-induced both targeted and untargeted cytogenetic effects. Such effects can be considered as risk factors for malignant transformation of cells, hereditary diseases, birth defects, and multifactorial somatic pathology. This article is a part of a Special Issue entitled “The Chornobyl Nuclear Accident: Thirty Years After”.

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LONG-TERM GENETIC AND EPIGENETIC DISORDERS IN PERSONS EXPOSED TO IONIZING RADIATION AND THEIR DESCENDANTS (review)

Kucher¹,

Vydyborets²

2021

Probl Radiac Med Radiobiol

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The review is devoted to long-term genetic and epigenetic disorders in exposed individuals and their descendants, namely to cytogenetic effects in the Chornobyl NPP accident clean-up workers and their children, DNA methylation as an epigenetic modification of human genome. Data presented in review expand the understanding of risk of the prolonged exposure for the present and future generations, which is one of key problems posed by fundamental radiation genetics and human radiobiology. The Scientific Council meeting of NAMS approved the NRCRM Annual Report. Key words: Chornobyl NPP accident, cytogenetic effects, DNA methylation.

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Radiation-induced modification of the chromosome sensitivity of human somatic cells to the testing mutagenic exposure of bleomycin in vitro

Cited by 4 publications

References 8 publications

Individual Sensitivity of the Peripheral Blood Lymphocytes to the in Vitro Action of Mitomycin C in Children with Juvenile Rheumatoid Arthritis

Individual Sensitivity of the Peripheral Blood Lymphocytes to the in Vitro Action of Mitomycin C in Children with Juvenile Rheumatoid Arthritis

Chromosomal Mutagenesis in Human Somatic Cells: 30-Year Cytogenetic Monitoring After Chornobyl Accident

LONG-TERM GENETIC AND EPIGENETIC DISORDERS IN PERSONS EXPOSED TO IONIZING RADIATION AND THEIR DESCENDANTS (review)

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